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Novel high-efficiency composite hemostatic sponge and preparation method thereof

A hemostatic sponge and high-efficiency technology, applied in the field of composite hemostatic sponge and its preparation, can solve the problems of long hemostasis time, insufficient pressure, no anti-inflammatory effect, etc., and achieve the effects of promoting tissue healing, effective hemostasis, and rapid in vivo degradation.

Inactive Publication Date: 2016-11-23
SHANGXIAN MINIMAL INVASIVE INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, gelatin sponge is applied to the wound, the material is soft, and the pressure is insufficient, so the hemostasis time is slightly longer, and it has no anti-inflammatory effect, so it cannot control the infection in the alveolar
Moreover, its biodegradation rate is relatively fast, so it is not suitable for implantation in the body.

Method used

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  • Novel high-efficiency composite hemostatic sponge and preparation method thereof
  • Novel high-efficiency composite hemostatic sponge and preparation method thereof
  • Novel high-efficiency composite hemostatic sponge and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Preparation of collagen sponge

[0035] 1) Degreasing: Scrape off the fascia and grease on the surface of fresh beef Achilles tendon, soak and wash with distilled water, chop it up, and put it into a tissue masher to mash and homogenate. Soak in 0.1% sodium carbonate solution for 12 hours, and filter to obtain fat-removed bovine Achilles tendon.

[0036] 2) Extraction: Take 10 g of the fat-removed bovine Achilles tendon obtained above and put it into a three-neck flask, add 0.45 g of pepsin and about 1000 ml of 0.3% acetic acid solution. Stir at 0-4°C for 3 days. The obtained crude extract was centrifuged in a high-speed refrigerated centrifuge at a speed of 10,000 rpm for 20 min, and the supernatant was taken.

[0037] 3) Salting out: Add NaCl particles to the supernatant after centrifugation, under stirring, until the concentration of sodium chloride reaches 2-3M, wait for 12-18 hours after precipitation, filter, and get a white viscous precipitate .

[0038] ...

Embodiment 2

[0045] (1) Preparation of Collagen Sponge: Same as Example 1

[0046] (2) Preparation of composite hemostatic sponge

[0047] 1) Preparation of composite material Col-ORC: 0.06 g of collagen sponge (Col) was added to every 10 ml of acetic acid aqueous solution (1% v / v) to prepare collagen acetic acid aqueous solution. Take 100ml of collagen acetic acid aqueous solution, add oxidized regenerated cellulose (ORC), the amount of oxidized regenerated cellulose (ORC) is 0.5% of the collagen protein, at room temperature, stir magnetically until the powder is evenly dispersed, then freeze dry for 24-48h , to obtain the composite material Col-0.5% ORC of collagen and oxidized regenerated cellulose.

[0048] 2) Take 25mg of composite material Col-0.5% ORC, put it in 10ml ethanol / MES buffer solution (40% v / v), soak for 30min, filter, discard the filtrate to get the filtrate; then according to the material-liquid ratio of 1:3.5, take 1 mg of filtrate and 3.5 ml of ethanol / MES buffer (40% ...

Embodiment 3

[0051] (1) Preparation of Collagen Sponge: Same as Example 1

[0052] (2) Preparation of composite hemostatic sponge

[0053] 1) Preparation of composite material Col-ORC: 0.06 g of collagen sponge (Col) was added to every 10 ml of acetic acid aqueous solution (1% v / v) to prepare collagen acetic acid aqueous solution. Take 100ml of collagen acetic acid aqueous solution, add oxidized regenerated cellulose (ORC), the amount of oxidized regenerated cellulose (ORC) is 1.0% of the collagen protein mass, stir magnetically at room temperature until the powder is evenly dispersed, freeze-dry for 24-48h, The composite material Col-1% ORC of collagen and oxidized regenerated cellulose was obtained.

[0054] 2) Take 25mg of the composite material Col-0.25% ORC, put it in 10ml ethanol / MES buffer (40% v / v), soak for 30min, filter, discard the filtrate to get the product; then take 1mg according to the ratio of material to liquid 1:3.5 Filtrate and 3.5ml ethanol / MES buffer (40% v / v), afte...

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Abstract

The invention relates to a novel high-efficiency composite hemostatic sponge and a preparation method thereof. According to the technical scheme, the preparation method comprises the following steps: dissolving Col in acetic acid solution, adding a proper amount of ORC, magnetically stirring at normal temperature until the powder is uniformly dispersed, and performing freeze drying, thereby obtaining a composite material Col-ORC; soaking the composite material Col-ORC in 15-45% ethanol / MES buffer solution, filtering, and removing the filtrate, thereby obtaining a filtered material; taking the filtered material and ethanol / MES buffer solution according to a material-liquid ratio of 1:(2.5-4.5), soaking for 3-6 hours, slowly adding EDC and NHS, reacting for 4-8 hours, neutralizing excessive EDC and NHS by using 1M disodium hydrogen phosphate, filtering, removing the filtrate, and washing by deionized water, thereby obtaining a cross-linked product. The hemostatic sponge prepared by the invention accelerates hemostatic process, does not have biotoxicity and can promote tissue healing.

Description

technical field [0001] The invention belongs to the field of medical hemostatic sponges, in particular to a composite hemostatic sponge prepared by cross-linking collagen and oxidized regenerated cellulose and a preparation method thereof. Background technique [0002] Hemostasis is critical to the success of oral and maxillofacial surgery, especially in patients with complications after orthognathic surgery and in patients with bleeding tendencies such as thrombocytopenia. With the development of hemostasis methods, many packing and suturing methods are commonly used in clinic. A number of tamponade hemostatic materials, including gelatin sponge, chitin, and chitosan sponge, are used in surgery, each with advantages and limitations. For example, gelatin sponge is applied to the wound, the material is soft, and the pressure is insufficient, which makes the hemostasis time slightly longer, and has no anti-inflammatory effect, so it cannot control the infection in the alveola...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L24/10A61L24/08A61L24/00
CPCA61L24/102A61L24/001A61L24/0036A61L24/0042A61L24/08A61L2400/04C08L89/04C08L1/04
Inventor 程玮璐李慧李洪谊李贵阳王耀涓刘富忠赵志平高健郭凯蔡盼盼刘杰
Owner SHANGXIAN MINIMAL INVASIVE INC
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