Molecular biological detection method for type 3, 4, 5, 8, 10, 16, 19, 23 and 25 serotypes of streptococcus suis
A technology of molecular biology and Streptococcus suis, applied in biochemical equipment and methods, microbiological determination/inspection, etc., can solve problems such as time-consuming, difficult to observe, and molecular biological detection methods for serotypes have not yet been established
Inactive Publication Date: 2016-11-23
CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT
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The serotype identification of Streptococcus suis has been using time-consuming and difficult-to-observe serum agglutination or synergistic agglutination methods, except for Smith et al. Molecular biological detection method, the other serotypes have not yet established a simple and rapid molecular biological detection method
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[0011] The following examples illustrate the technical solution of the present invention, but the protection scope of the present invention is not limited to this example.
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The invention belongs to the technical field of biology, and provides a molecular biological detection method for 9 (type 3, 4, 5, 8, 10, 16, 19, 23 and 25) serotypes of streptococcus suis. The molecular biological detection method has three technical points that 1, a primer sequence required by nucleic acid detection is determined; 2, detection reaction kinds are determined; 3, a detection reaction system and reaction conditions are determined. The detection method can be applied to scientific research of 9 serotype strains of the streptococcus suis, and can be applied to clinical diagnosis and detection of animals or human beings.
Description
technical field [0001] The invention belongs to the field of biotechnology; specifically, the invention establishes a molecular biology detection method for 9 serotypes (types 3, 4, 5, 8, 10, 16, 19, 23, and 25) of Streptococcus suis, using The identification and detection of the 9 serotype strains of Streptococcus suis have great application value in scientific research, medical clinical diagnosis and veterinary clinical diagnosis. Background technique [0002] Streptococcus suis can cause a variety of clinical diseases in pigs and other livestock. In the early stage, it can be divided into 35 serotypes (1-34, 1 / 2) according to the difference of capsule antigen; in 2005, Nill et al. and type 34 are murine oral streptococci ( Streptococcus orisratti ). The serotype identification of Streptococcus suis has been using time-consuming and difficult-to-observe serum agglutination or synergistic agglutination methods, except for Smith et al. Molecular biological detection met...
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Login to View More IPC IPC(8): C12Q1/68C12Q1/14
CPCC12Q1/689C12Q2600/16
Inventor 王楷宬陆承平朱琳
Owner CHINA ANIMAL HEALTH & EPIDEMIOLOGY CENT