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A method for measuring the concentration of 8-epiflavin e-acetate in blood plasma

A technology of flavonoids and acetate, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effects of simple operation, high sensitivity and rapid method

Active Publication Date: 2018-08-31
JIANGSU PROVINCIAL HOSPITAL OF TCM
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] There is no literature report on the in vivo determination method of 8-epiflavin E acetate

Method used

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  • A method for measuring the concentration of 8-epiflavin e-acetate in blood plasma
  • A method for measuring the concentration of 8-epiflavin e-acetate in blood plasma
  • A method for measuring the concentration of 8-epiflavin e-acetate in blood plasma

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Experimental program
Comparison scheme
Effect test

Embodiment

[0028] Embodiment: Determination of 8-epiflavin E acetate concentration in rat plasma

[0029] 1. Experimental materials and instruments

[0030] 8-Epiflavin E acetate: isolated from the dry tuber of Huangdu; tinidazole (internal standard): purchased from China National Institute for the Control of Pharmaceutical and Biological Products, batch number: 100336-200703; test water: ultrapure water; methanol: chromatographic Pure (Merck Company).

[0031] Agilent G6430LC-MS / MS liquid mass spectrometry instrument (Agilent Company of the United States, equipped with high performance liquid phase instrument is Agilent 1290); MassHunter data processing system (version B.05.00); WH-2 micro vortex mixer (Shanghai Huxi Analytical Instrument Factory); AE240 electronic balance (Shanghai Mettler-Toledo Co., Ltd.); Millipore Drict-Q5 pure water machine (Millipore, France); Biofuge PrimoR refrigerated high-speed centrifuge (Heraeus, Germany).

[0032] 2. Liquid conditions

[0033] 1. Liquid...

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Abstract

The invention discloses a method for determining the concentration of 8-epiflavin E acetate in plasma. The method adopts a liquid chromatography-mass spectrometry system for determination. First, a sample to be tested is taken, a certain amount of an organic solvent protein precipitation agent is added to carry out protein precipitation, and after pretreatment , separated by a chromatographic column and detected by a mass spectrometer detector, the method of the invention is fast, accurate, highly sensitive, easy to operate, and suitable for determining the concentration of 8-epiflavin E acetate in plasma.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for measuring the concentration of 8-epiflavin E acetate in blood plasma. Background technique [0002] 8-epidiosbulbin E acetate is a new furan-n-diterpene compound isolated from the dry tuber of Dioscorea bulbifera L., a perennial herb of the Dioscorea family. In vitro experiments have proved that 8-epiflavin E acetate has significant anti-tumor, anti-fungal, anti-inflammatory and other biological activities, and is a new drug source compound with good development prospects. Its structural formula is as follows: [0003] [0004] There is no literature report on the in vivo determination method of 8-epiflavin E acetate. The invention establishes a method for measuring 8-epiflavin E acetate in rats, and conducts exploratory research on drug metabolism of 8-epiflavine E acetate through the method. Contents of the invention [0005] The te...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027G01N2030/065
Inventor 戴国梁张农山刘史佳居文政吴磊张倩李长印
Owner JIANGSU PROVINCIAL HOSPITAL OF TCM
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