Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A Method for Super-resolution Dipole Orientation Analysis

A dipole and super-resolution technology, applied in the field of super-resolution imaging, can solve problems such as low time resolution and limited observation ability of living cells, and achieve the effects of improving accuracy, anti-noise and stability, and improving effectiveness

Active Publication Date: 2018-09-14
PEKING UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different wavelengths have strict requirements on fluorescent markers, which restricts the application. At the same time, the time resolution is too low (several minutes), which limits the ability to observe living cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Method for Super-resolution Dipole Orientation Analysis
  • A Method for Super-resolution Dipole Orientation Analysis
  • A Method for Super-resolution Dipole Orientation Analysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0029] The invention provides a method for realizing super-resolution dipole orientation analysis. The method is based on the fact that fluorescent proteins have dipole characteristics, and then uses fluorescent proteins to mark target proteins. Under the condition that the two proteins form a rigid structure, by exciting to study fluorescent protein dipole orientations, allowing analysis of structural changes in the target protein. The present invention specifically comprises the following steps:

[0030] 1) if figure 1As shown, a micro-imaging system is set, and the micro-imaging system adds a half-wave plate on the basis of a wide-field fluorescence microscope (epi-fluorescence microscope), which includes a laser light source 1, a half-wave plate 2, a collimator lens 3, two Chromatic mirror 4, objective lens 5, reflecting mirror 6, converging len...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for realizing super-resolution dipole orientation analysis. The method comprises the following steps: a microscopic imaging system is arranged, wherein the system comprises a laser light source, a half-wave plate, a collimating lens, a dichroscope, an object lens, a reflector, a convergent lens, and an EMCCD imaging device; light splitting difference correction for different polarized light due to diffraction, background noise, and the dichroscope as well as Poisson sampling property factors of EMCCD acquisition signals are considered, a fluorescent polarization modulation microscopic imaging process is modeled, and a super-resolution intensity signal is obtained; after counter-demodulation, polarization modulation properties of singles in a single pixel are used, least square fitting is carried out for the super-resolution intensity signal, average dipole orientation in a single pixel is extracted, and signals with super-diffraction limiting resolution are realized; a direction consistency factor OUF is defined for evaluating validity of average dipole direction after reconstruction; and two visualization methods of super-resolution signals are used, dimensions for distinguishing different molecules are added from the aspect of an angle resolution, and fine understanding of protein compositions is realized.

Description

technical field [0001] The invention relates to a super-resolution imaging method, in particular to a method for realizing super-resolution dipole orientation analysis. Background technique [0002] Fluorescent labeling microscopy is widely used to observe the subcellular structure of biological samples. Fluorescence has four characteristics: intensity (intensity, reflecting fluorescence density), wavelength (wavelength, absorption and emission spectrum), time (time, fluorescence decay lifetime) and polarization (polarization, caused by dipole orientation). The dipole orientation of fluorescent molecules can reflect the spatial orientation of the target protein, and then study the structure and dynamics of the target protein in living cells. [0003] Fluorescence polarization microscopy (FPM) measures the orientation of dipoles by analyzing the polarization properties to study the orientation of the target protein. FPM is already a relatively mature optical imaging system,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6458G01N2201/06113
Inventor 陈龙张昊杨旭三王淼妍张奇伟高军涛席鹏
Owner PEKING UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com