Method for rapidly obtaining and purifying human umbilical cord mesenchymal stem cells
A technology of stem cells and human umbilical cord, applied in the biological field, can solve the problems of long passage of stem cells, unsatisfactory cell adherence ability and proliferation ability, and unsatisfactory speed.
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[0053] 1. Obtain the umbilical cord of the newborn
[0054] Under sterile conditions, the umbilical cord tissue of a healthy newborn with a length of 45 cm was obtained from the junction of the umbilical cord and the placenta, washed repeatedly with PBS to remove blood stains, and the vascular tissue in the umbilical cord was carefully removed by mechanical separation with a microscopic instrument to obtain Wharton's glue, cut it into 2mm 3 Left and right pieces.
[0055] 2. Obtain P1 generation hUMSCs
[0056] 1) After washing the obtained Wharton's jelly fragments with PBS again, resuspend them in DMEM culture medium with a volume fraction of 25% FBS, inoculate them in 10 cm petri dishes, and then incubate at 37°C, 5% CO 2 , 95% humidity conditions, incubated in the incubator for 84h to obtain adherent cells ( figure 1 ).
[0057] 2) Discard the culture medium and suspended cells, wash with PBS, add 0.125% compound collagenase to cover the bottom of the dish, digest in a...
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