Method for optimizing extraction of total flavonoids from ziziphus jujube with response surface method

A technology of response surface method and total flavonoids, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant raw materials, etc., can solve the research that restricts the pharmacology and quality control of total flavonoids of kasikapu jujube, kasikapu Problems such as low purity of jujube total flavonoids

Inactive Publication Date: 2016-12-21
XINJIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the purity of the total flavonoids obtained by the current extraction method is generally low, which has always restricted the research on the pharmacology and quality control of the total flavonoids of kasikapu

Method used

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  • Method for optimizing extraction of total flavonoids from ziziphus jujube with response surface method
  • Method for optimizing extraction of total flavonoids from ziziphus jujube with response surface method
  • Method for optimizing extraction of total flavonoids from ziziphus jujube with response surface method

Examples

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Effect test

example 1

[0057] The extraction method of the total flavonoids of kasikafu jujube, the steps are as follows:

[0058] Carry out selection, cleaning, slicing, pitting, and drying of kasikapu jujube, coarsely crush the dried kasikapu jujube into 100-mesh granules, weigh 9.9322 g of kasikapu jujube powder, press 1 : 25 (g / mL) solid-liquid ratio, add absolute ethanol, place in a 70°C constant temperature water bath for extraction for 1.5h, centrifuge, take the supernatant to obtain a crude extract; use a rotary evaporator to concentrate the extract, and Dry in a vacuum oven for 24 hours. Dissolve the dry extract in anhydrous methanol, and make the volume into a 50ml volumetric flask, and use NaNO 2 -Al(NO 3 ) 3 The content of total flavonoids in Kashikafu jujube was determined to be 1.3748 mg / g by chromogenic ultraviolet spectrophotometry.

example 2

[0060] The extraction method of the total flavonoids of kasikafu jujube, the steps are as follows:

[0061] Carry out selection, cleaning, slicing, pitting, and drying of kasikapu jujube, coarsely crush the dried kasikapu jujube into 100-mesh particles, weigh 10.8135 g of kasikapu jujube powder, press 1 : 30 (g / mL) ratio of solid to liquid, add absolute ethanol, place in 80°C constant temperature water bath for extraction for 1.5h, centrifuge, take the supernatant to obtain the crude extract; use a rotary evaporator to concentrate the extract, And use a vacuum oven to dry, and the drying time is 24 hours. Dissolve the dry extract in anhydrous methanol, and make the volume into a 50ml volumetric flask, and use NaNO 2 -Al(NO 3 ) 3 The content of total flavonoids in Kashikafu jujube was determined to be 1.3272 mg / g by chromogenic ultraviolet spectrophotometry.

example 3

[0063] The extraction method of the total flavonoids of kasikafu jujube, the steps are as follows:

[0064] Carry out selection, cleaning, slicing, pitting, and drying of kasikapu jujube, coarsely crush the dried kasikapu jujube into 100-mesh particles, weigh 10.0696 g of kasikapu jujube powder, press 1 : 25 (g / mL) ratio of solid to liquid, add absolute ethanol, place in 80°C constant temperature water bath for leaching for 2.0h, centrifuge, take supernatant to obtain crude extract; use rotary evaporator to concentrate the extract, And use a vacuum oven to dry, and the drying time is 24 hours. Dissolve the dry extract in anhydrous methanol, and make the volume into a 50ml volumetric flask, and use NaNO 2 -Al(NO 3 ) 3 The content of total flavonoids in Kashikafu jujube was determined to be 1.2865 mg / g by chromogenic ultraviolet spectrophotometry.

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Abstract

The invention discloses a method for optimizing the extraction of total flavonoids from ziziphus jujube with a response surface method. The method comprises the following steps: enucleating, drying and crushing ziziphus jujube to obtain ziziphus jujube powder; extracting the ziziphus jujube powder with ethanol; measuring the content of total flavonoids in an extracting solution; selecting three factors including extraction time, extraction temperature and liquid-to-solid ratio to perform single-factor experiments; determining optimal levels of the extraction time, extraction temperature and liquid-to-solid ratio; symmetrically selecting two levels at both sides of each optimal level; carrying out three-factor three-level experiment design according to a Box-Benhnken design principle; establishing multivariate quadratic equations respectively by using the extraction time, extraction temperature and liquid-to-solid ratio as independent variables and using the content of flavonoid as a response value; and performing response surface analysis on the multivariate quadratic equations; taking the content of total flavonoids as a maximum value to obtain optimal extraction conditions. The invention adopts the response surface method to optimize the extraction process, and obtains the high-purity total flavonoids of ziziphus jujube.

Description

technical field [0001] The invention relates to a method for optimally extracting total flavonoids from kasikafu jujube by response surface method. Background technique [0002] Kasikapu jujube, also known as sour jujube and small red jujube, is the dry and mature fruit of Ziziphus jujuba Mill.var.spinosa (Bunge) Hu ex.H.F.Chow, a plant of Rhamnaceae. It has the effects of clearing blood and detoxifying, clearing heat and reducing inflammation, relieving cough and reducing phlegm, nourishing the body and calming the nerves, removing rash and relieving itching. It is used for various diseases of febrile or hemorrhagic or biliary nature, such as blood heat putrefaction, concentrated liquid clumping, fever, pneumonia, pleurisy, cold due to cold, cough with persistent phlegm, physical weakness, insomnia, rash itching etc. [0003] The efficacy of flavonoids is multifaceted. It is a strong antioxidant that can effectively remove oxygen free radicals in the body. For example, an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/725A61P35/00
CPCA61K36/725A61K2236/33A61K2236/51
Inventor 哈木拉提·吾甫尔艾尼娃尔·艾克木
Owner XINJIANG MEDICAL UNIV
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