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Regulation method for humification parameter in composting water-soluble organic matter

A water-soluble organic matter, humification technology, applied in biochemical equipment and methods, microbial determination/inspection, color/spectral property measurement, etc. , to save time, enhance activity, and promote humification.

Inactive Publication Date: 2016-12-21
CHINESE RES ACAD OF ENVIRONMENTAL SCI
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Problems solved by technology

[0003] However, according to the traditional culture method of microorganisms, it is impossible to extract the key flora in compost, and the cultivable microorganisms in compost only account for 0.1-10% of the total microorganisms, so it is difficult to separate and purify key microorganisms

Method used

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  • Regulation method for humification parameter in composting water-soluble organic matter
  • Regulation method for humification parameter in composting water-soluble organic matter
  • Regulation method for humification parameter in composting water-soluble organic matter

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Embodiment 1

[0053] The present invention takes the compost sample collected by a garbage factory in Beijing as an example. In a domestic garbage composting factory in Beijing, collect compost raw materials, samples of composting 0, 7, 14, 21, 28, 51 and 90 days, respectively marked as C0, For C7, C14, C21, C28, C51, and C90, add double-distilled water to the sample at a solid-to-liquid ratio of compost dry weight 1:10 (g / ml), shake for 8 hours, and take the supernatant to pass through a 0.45 μm filter membrane after centrifugation , to produce compost water-soluble organic matter. Samples were extracted with a soil DNA extraction kit for total genomic DNA and stored at -20°C for later use.

[0054] Determination of characteristic UV and fluorescence spectral parameters. Measure the UV absorption value of water-soluble organic matter at 254nm and divide it by its WSC concentration to obtain the UVA value of water-soluble organic matter per unit concentration SUVA 254 ; Measure the area v...

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Abstract

The invention relates to a regulation method for the humification parameter in a composting water-soluble organic matter. The main steps comprise: (1) collecting a sample and extracting a water-soluble organic matter; (2) determining the ultraviolet-visible absorption spectrum and three-dimensional fluorescence spectrum of the water-soluble organic matter, and extracting the characteristic humification parameter; (3) extracting the total DNA of bacteria, carrying out PCR amplification and denatured gradient gel electrophoresis (DGGE), cutting the target band from the gel, and sequencing; (4) based on the interrelation between the bacterial population structure and the water-soluble organic matter humification parameter, obtaining the key microorganism affecting the water-soluble organic matter humification by using canonical correspondence analysis; and (5) determining the regulation method of the key microorganism by combining the interrelation between the environmental factor and the key microorganism so as to achieve the indirect promotion of the composting water-soluble organic matter humification by regulating the environmental factor. According to the present invention, the analysis is simple, the technical requirement is low, and the convenient and feasible molecular biological means is provided for the promotion of the compost humification.

Description

technical field [0001] The invention belongs to the field of solid organic waste treatment and resource utilization, and specifically relates to a method for regulating humification parameters in composting water-soluble organic matter. Background technique [0002] With the progress and development of society, the amount of domestic garbage and organic solid waste produced by human beings in production and life is increasing day by day. Composting is an important means of waste reduction and resource utilization, and municipal solid waste composting has become a developing new industry. The key to the quality of composting lies in the optimization of physical and chemical parameters in the composting process. Proper optimization can ensure the smooth progress of composting and improve composting efficiency. The humification of water-soluble organic matter is of great significance to the stability of compost. The essence of aerobic composting is the physiological and bioch...

Claims

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Application Information

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IPC IPC(8): G01N21/33G01N21/64C12Q1/68C12Q1/04
CPCG01N21/33C12Q1/689G01N21/64
Inventor 席北斗赵昕宇何小松高如泰张慧李丹黄彩红檀文炳
Owner CHINESE RES ACAD OF ENVIRONMENTAL SCI
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