Pharmaceutical composition of carmustine and its application in biomedicine

A carmustine and composition technology, applied to pharmaceutical compositions and their application fields in biomedicine, can solve problems such as no related reports on carmustine, and achieve the effect of improving hypoglycemic effect

Pending Publication Date: 2016-12-28
黄亦琼
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, there has been no report on the correlation bet

Method used

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  • Pharmaceutical composition of carmustine and its application in biomedicine
  • Pharmaceutical composition of carmustine and its application in biomedicine
  • Pharmaceutical composition of carmustine and its application in biomedicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Compound (I) Separation Preparation and Structure Confirmation

[0020] Separation method: (a) Grind leek seeds (2kg), extract with 70% ethanol under hot reflux (15L×3 times), combine the extracts, concentrate until no alcohol smell (3L), and then use petroleum ether (3L×3 times) ), ethyl acetate (3L × 3 times) and water-saturated n-butanol (3L × 3 times) extraction, respectively obtain petroleum ether extract, ethyl acetate extract and n-butanol extract; (b) step (a ) in ethyl acetate extract with D101 type macroporous resin to remove impurities, first eluted with 20% ethanol for 9 column volumes, then eluted with 65% ethanol for 10 column volumes, collected 65% eluate, concentrated under reduced pressure Obtain 65% ethanol elution concentrate; (c) in step (b), 65% ethanol elution concentrate is separated with normal phase silica gel, is 65:1 (9 column volumes), 35:1 (10 column volumes) successively with volume ratio column volume), 15:1 (8 column volumes) ...

Embodiment 2

[0024] Embodiment 2: pharmacological action

[0025] 1. Materials and methods

[0026] 1.1 Materials and Instruments

[0027] Healthy male ICR mice, animal experiment center of Shenyang Medical College, weighing 18-22 g; carmustine was purchased from China Institute for the Control of Pharmaceutical and Biological Products. Compound (I) is self-made, and the preparation method is shown in Example 1. Streptozotocin (STZ) Sigma Chemical Company of the United States, made 1% STZ solution with citric acid buffer before use; other reagents were of domestic analytical grade. SW-CJ-2FD ultra-clean workbench Suzhou Purification Equipment Co., Ltd.; QYC-2112 large-scale double-layer full-temperature culture shaker Shanghai Tiancheng Experimental Instrument Manufacturing Co., Ltd.; SHY-2A dual-function water bath constant temperature oscillator Jiangsu Dongpeng Instrument Manufacturing Co., Ltd. ; TGL20W desktop high-speed refrigerated centrifuge Hunan Xiangyi Laboratory Instrument D...

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PUM

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Abstract

The invention discloses a pharmaceutical composition of carmustine and its application in biomedicine. The pharmaceutical composition of carmustine provided by the invention contains carmustine and a novel natural product compound (I ), the combination of carmustine and compound (I) can significantly improve the glucose tolerance of diabetic model mice, inhibit the activity of α-glucosidase, and improve the anti-oxidative damage ability of diabetic model mice. The activity of the combination of carmustine and compound (I) is stronger than the activity of carmustine or compound (I) alone, and can be developed into a drug for treating diabetes. Compared with the prior art, it has outstanding substantive characteristics and significant improvement.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a new application of carmustine, in particular to a pharmaceutical composition of carmustine and its application in biomedicine. Background technique [0002] Carmustine and its metabolites can cross-link with nucleic acids through alkylation, and may also produce anticancer effects by changing proteins. It can interact with DNA polymerase in vivo, and has effects on all phases of proliferating cells. [0003] So far, there has been no report on the correlation between carmustine and its pharmaceutical composition and diabetes. Contents of the invention [0004] The purpose of the present invention is to provide a pharmaceutical composition of carmustine, which contains carmustine and a natural product with novel structure, and carmustine and the natural product can synergistically treat diabetes. [0005] Above-mentioned purpose of the present invention is achieved by following techn...

Claims

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Application Information

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IPC IPC(8): C07J71/00A61K31/585A61K31/175A61P3/10
Inventor 黄亦琼
Owner 黄亦琼
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