Pharmaceutical composition of carmustine and its application in biomedicine
A carmustine and composition technology, applied to pharmaceutical compositions and their application fields in biomedicine, can solve problems such as no related reports on carmustine, and achieve the effect of improving hypoglycemic effect
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Embodiment 1
[0019] Example 1: Compound (I) Separation Preparation and Structure Confirmation
[0020] Separation method: (a) Grind leek seeds (2kg), extract with 70% ethanol under hot reflux (15L×3 times), combine the extracts, concentrate until no alcohol smell (3L), and then use petroleum ether (3L×3 times) ), ethyl acetate (3L × 3 times) and water-saturated n-butanol (3L × 3 times) extraction, respectively obtain petroleum ether extract, ethyl acetate extract and n-butanol extract; (b) step (a ) in ethyl acetate extract with D101 type macroporous resin to remove impurities, first eluted with 20% ethanol for 9 column volumes, then eluted with 65% ethanol for 10 column volumes, collected 65% eluate, concentrated under reduced pressure Obtain 65% ethanol elution concentrate; (c) in step (b), 65% ethanol elution concentrate is separated with normal phase silica gel, is 65:1 (9 column volumes), 35:1 (10 column volumes) successively with volume ratio column volume), 15:1 (8 column volumes) ...
Embodiment 2
[0024] Embodiment 2: pharmacological action
[0025] 1. Materials and methods
[0026] 1.1 Materials and Instruments
[0027] Healthy male ICR mice, animal experiment center of Shenyang Medical College, weighing 18-22 g; carmustine was purchased from China Institute for the Control of Pharmaceutical and Biological Products. Compound (I) is self-made, and the preparation method is shown in Example 1. Streptozotocin (STZ) Sigma Chemical Company of the United States, made 1% STZ solution with citric acid buffer before use; other reagents were of domestic analytical grade. SW-CJ-2FD ultra-clean workbench Suzhou Purification Equipment Co., Ltd.; QYC-2112 large-scale double-layer full-temperature culture shaker Shanghai Tiancheng Experimental Instrument Manufacturing Co., Ltd.; SHY-2A dual-function water bath constant temperature oscillator Jiangsu Dongpeng Instrument Manufacturing Co., Ltd. ; TGL20W desktop high-speed refrigerated centrifuge Hunan Xiangyi Laboratory Instrument D...
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