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A kind of tissue culture rapid propagation method of scarlet Sarracenia

A technique for tissue culture and rapid propagation of Sarracenia, applied in the field of tissue culture and rapid propagation of Scarlet Sarracenia

Active Publication Date: 2018-09-25
吴子平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few related research reports at home and abroad, mainly to induce callus from shoot tips and leaves, and then induce callus to differentiate into adventitious buds, and then form complete plants from adventitious buds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] ① Selection of explants: Select the robust and pest-free Scarlet Sarracenia as the mother plant, take the stem segment with terminal buds as the explant, remove the leaves and outer bracts, and set aside.

[0023] ② Disinfection of explants: On the ultra-clean workbench, put the stems with terminal buds into sterile empty bottles, use 0.1% mercury liter as the disinfectant, disinfect for 8 minutes, and then rinse with sterile water twice. The sterility rate was 81.1%.

[0024] ③Callus induction and adventitious bud differentiation: Insert the sterilized stem segment with terminal buds into the induction and differentiation medium; 1 stem segment / cup, 26±1°C, 300~1800lux culture, light 12h / d, 1 transfer per month. Differentiation medium: MS 改 +2.5mg / L 6-BA+0.05mg / LNAA+30g / L sucrose+5.5g / L agar, pH6.0. The terminal buds began to germinate after 18 days of cultivation, and after 27 days of cultivation, the terminal buds grew, and new shoots / leaves grew from the stems. ...

Embodiment 2

[0029] ① Selection of explants: Select the robust and pest-free Scarlet Sarracenia as the mother plant, take the stem segment with terminal buds as the explant, remove the leaves and outer bracts, and set aside.

[0030] ② Disinfection of explants: On the ultra-clean workbench, put the stems with terminal buds into sterile empty bottles, use 0.1% mercury liter as the disinfectant, disinfect for 7 minutes, and then rinse with sterile water for 3 times. The sterility rate was 82.3%.

[0031] ③Callus induction and adventitious bud differentiation: Insert the sterilized stem segment with terminal buds into the induction and differentiation medium; 1 stem segment / cup, 26±1°C, 300~1800lux culture, light 12h / d, 1 transfer per month. Differentiation medium: MS 改 +3mg / L 6-BA+0.1mg / LNAA+30g / L sucrose+5.5g / L agar, pH6.0. The terminal buds began to germinate after 20 days of cultivation, and after 30 days of cultivation, the terminal buds grew, and new shoots / leaves grew from the stem....

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PUM

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Abstract

The invention belongs to the field of plant tissue culture, and particularly relates to a tissue culture and rapid propagation method of bloodred sarracenia. The method comprises the following steps: using a stem of a strong parent plant with an apical bud as an explant, (1) sterilizing the explant for 6 to 8 min with 0.1 percent liter of mercury, (2) inoculating the sterilized apical bud in an induction and differential medium with 6-BA and NAA for culturing, (3) inoculating callus or adventitious bud blocks into a proliferation culture medium with 6-BA, BR, PP333 and NAA, and (4) inoculating sprouts with 6 to 15 leaves (more than or equal to 5 leaves with the width greater than or equal to 2mm) and with a height greater than or equal to 40mm into a rooting culture medium with active carbon. By adopting the tissue culture and rapid propagation method, a great amount of asexual reproduced bloodred sarracenia tissue culture seedlings can be obtained within a short time.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of Sarracenia sarracenia. Background technique [0002] Scarlet Sarracenia is a perennial herbaceous carnivorous plant of the Sarraceae family Sarracenia, which is a horticultural hybrid. Scarlet Sarracenia has a very beautiful appearance, the leaves are tube-shaped, upright and angular, and there are scarlet net patterns on the mouth of the leaf tube and the cover of the tube. Scarlet Sarracenia can trap insects. Its leaf tube has a small opening, and the inner wall can secrete honey juice and digestive juice to attract and prey on insects. It is one of the most popular interesting plants in the world today, with high ornamental value and economic value. [0003] At present, the propagation methods of Sarracenia mainly include division, sowing and tissue culture. [0004] Propagate by division. After removing th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 吴子平纪超群
Owner 吴子平