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Tissue culture rapid propagation method for purple pitcher plant

A technique for tissue culture and rapid propagation of bottle grass, applied in the field of tissue culture and rapid propagation of purple bottle grass

Inactive Publication Date: 2016-11-09
吴子平
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few related research reports at home and abroad, mainly to induce callus from shoot tips and leaves, and then induce callus to differentiate into adventitious buds, and then form complete plants from adventitious buds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] ① Selection of explants: Select a robust purple Sarracenia that is free from diseases and insect pests as the mother plant, take its stem segment with terminal buds as the explant, remove the leaves and outer bracts, and set aside.

[0024] ②Disinfection of explants: On the ultra-clean workbench, put the stems with terminal buds into sterile empty bottles, use 0.1% mercury liter as the disinfectant, disinfect for 10 minutes, and then rinse with sterile water twice. The sterility rate was 71.4%.

[0025] ③Callus induction and adventitious bud differentiation: Insert the sterilized stem segment with terminal buds into the induction and differentiation medium; 1 stem segment / cup, 26±1°C, 300~1800lux culture, light 12h / d, 1 transfer per month. Differentiation medium: MS 改 +2.5mg / L 6-BA+0.05mg / LNAA+30g / L sucrose+5.5g / L agar, pH6.0. The terminal buds began to germinate after 20 days of cultivation, and after 30 days of cultivation, the terminal buds grew, and new shoots / le...

Embodiment 2

[0030] ① Selection of explants: Select a robust purple Sarracenia that is free from diseases and insect pests as the mother plant, take its stem segment with terminal buds as the explant, remove the leaves and outer bracts, and set aside.

[0031] ② Disinfection of explants: On the ultra-clean workbench, put the stems with terminal buds into sterile empty bottles, use 0.1% mercury liter as the disinfectant, disinfect for 8 minutes, and then rinse with sterile water for 3 times. The sterility rate was 76.3%.

[0032] ③Callus induction and adventitious bud differentiation: Insert the sterilized stem segment with terminal buds into the induction and differentiation medium; 1 stem segment / cup, 26±1°C, 300~1800lux culture, light 12h / d, 1 transfer per month. Differentiation medium: MS 改 +3mg / L 6-BA+0.1mg / LNAA+30g / L sucrose+5.5g / L agar, pH6.0. The terminal buds began to germinate after 22 days of cultivation, and after 30 days of cultivation, the terminal buds grew, and new shoots...

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PUM

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Abstract

The invention belongs to the field of plant tissue culture and specifically relates to a tissue culture rapid propagation method for a purple pitcher plant. The method comprises the following steps of: taking a stem with a terminal bud of strong stock plant as explant; (1) disinfecting the explants with 0.1% mercury bichloride for 8-10min; (2) accessing the terminal bud after being disinfected to an inducing and differential medium added with 6-BA and NAA for culturing; (3) accessing the healing or uncertain bud block mass to an enrichment medium added with 6-BA, BR and NAA for culturing; and (4) accessing the bud block mass with 5-12 blades, plant height more than or equal to 35mm, more than or equal to 3 blades and blade width more than or equal to 3mm to a rooting culture medium added with active carbon for culturing. According to the tissue culture rapid propagation method for the purple pitcher plant provided by the invention, a large amount of vegetative propagated tissue culture seedlings of purple pitcher plant are acquired within a short period of time.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture and rapid propagation of Sarracenia violet. Background technique [0002] Purple Sarracenia is a perennial herbaceous carnivorous plant of the genus Sarracenia. The shape of Sarracenia is very beautiful, the leaves are bottle-shaped, lying on the side, bright in color, with gorgeous purple net pattern. Sarracenia can trap insects, and its cage can secrete honeydew and digestive juices to attract and prey on insects. It is one of the most popular interesting plants in the world today, with high ornamental value and economic value. [0003] At present, the propagation methods of Sarracenia mainly include division, sowing and tissue culture. [0004] Propagate by division. After removing the pot in spring, remove the rhizosphere matrix, and cut the rhizome into 3~4 clumps with a sharp knife, and each clump has at least a small bottle-sha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 吴子平纪超群
Owner 吴子平