Method for inducing cluster bud regeneration by means of blueberry stems with buds

A technology with buds and blueberries, which is applied in plant regeneration, horticultural methods, botanical equipment and methods, etc., can solve the problems affecting the expansion of blueberry planting area, low cutting propagation coefficient, supply can not keep up with demand, etc., to achieve the realization of industrial The effects of chemical seedling cultivation, good adaptability and easy operation

Inactive Publication Date: 2017-01-04
CHINA THREE GORGES CORPORATION
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Problems solved by technology

At present, due to the rapid expansion of blueberry planting area in my country, the supply of conventional cutting propagation seedlings cannot keep up with the dema

Method used

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  • Method for inducing cluster bud regeneration by means of blueberry stems with buds
  • Method for inducing cluster bud regeneration by means of blueberry stems with buds
  • Method for inducing cluster bud regeneration by means of blueberry stems with buds

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Experimental program
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Effect test

Embodiment 1

[0036] Embodiment 1: as Figure 1-6 as shown,

[0037] 1.1 Selection and treatment of mother plants

[0038] The test materials were selected from the three-year-old potted seedlings of blueberry (South Highbush) that grew robustly, had no pests and diseases, and had good shape in the greenhouse of the Three Gorges Nursery Research Center. 80cm. Treatment of the mother plant: In autumn and winter, when the outdoor temperature is lower than 10°C, heat the greenhouse and keep the greenhouse temperature at 18°C; in summer, when the outdoor temperature is higher than 30°C, cool the greenhouse and keep the greenhouse temperature at 25°C , the growth temperature of blueberries in the greenhouse is maintained at 18~25℃ all year round; when the sunlight is strong in summer, 75% sunshade nets are used for shading, and the light intensity is controlled at 2000 lx~3000 lx to prevent the blueberry pot seedlings from being burned by strong light; through spraying and ventilation The met...

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Abstract

The invention discloses a method for inducing cluster bud regeneration by means of blueberry stems with buds. The method comprises the specific steps that 1, a one-year-old robust coppice shoot free of pest and disease damage is selected as an explant, disinfected and then segmented, an inducing medium is inoculated with the segments, and after the segments are cultivated for 30 days, cluster buds are obtained and cut off for grafting, cultivation and proliferation; 2, seedlings which are cultured to 5 cm or above are segmented into single plants, a seedling strengthening and rooting medium is inoculated with the single plants, cultivation is conducted for 5 weeks or above, then seedling exercising is conducted, the seedlings are taken out, roots are cleaned, transplanting is conducted, and when the seedlings grow to 20-30 cm high, field planting is conducted; processing of cluster bud regeneration inducing by means of the blueberry stems with the buds is completed through the above-mentioned steps. According to the method, cultivation is easy, the propagation coefficient is high, the regeneration cycle is short, the regenerated plants can be continuously obtained, the cost is effectively reduced, and industrialized seedling cultivating can be achieved.

Description

technical field [0001] The invention relates to a plant tissue culture propagation technology, in particular to a method for inducing the regeneration of clustered buds from blueberry stem segments with buds. Background technique [0002] Blueberry belongs to Rhododendronaceae Vaccinium plant, which is a perennial deciduous or evergreen shrub or small shrub. Blueberry fruit is rich in nutrients and has functions such as preventing brain aging, protecting eyesight, strengthening heart, anti-cancer, softening blood vessels, and enhancing human immunity. Therefore, it is listed as one of the five health foods for human beings by the International Food and Agriculture Organization. The artificial cultivation of blueberries originated in North America, and my country started relatively late. Because its cultivation and growth have strict requirements on the natural environment and growth conditions, the industrial development of blueberry planting is limited. Among them, blueb...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01G9/10
CPCA01H4/001A01G24/00A01H4/008
Inventor 吴锦华张国禹黄桂云杨兰芳邱利文吴笛张海波马晓波胡梅香汪磊李翩翩
Owner CHINA THREE GORGES CORPORATION
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