Composite efficient microbial deodorizer and preparation method thereof

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a composite efficient microbial deodorizer and a preparation method thereof. The microbial deodorizer comprises candida utilis, bacillus subtilis, bacillus licheniformis, bacillus coagulans and enterococcus lactobacilli, viable count is 5.3*10<9>-8.3*10<9>cfu / g, viable count of the candida utilis is 1.8*10<8>-3.6*10<8>cfu / g, viable count of the bacillus subtilis is 3.4*10<8>-4.2*10<8>cfu / g, viable count of the bacillus licheniformis is 3.7*10<8>-7.4*10<8>cfu / g, viable count of the bacillus coagulans is 2.4*10<8>-3.6*10<8>cfu / g, and viable count of the enterococcus lactobacilli is 4.2*10<9>-6.4*10<9>cfu / g. The microbial deodorizer has strange deodorization effects and can rapidly and effectively remove odorous gas of pollution sources.

Description

technical field

[0001] The invention belongs to the technical field of microbes, and in particular relates to a composite high-efficiency microbial deodorant and a preparation method thereof. Background technique

[0002] The stench emitted by garbage disposal sites and transfer stations, sewage treatment plants, livestock and poultry farms, etc. has become an important source of pollution in urban and rural environments. The pollution of the odor generated by the environment is increasing, and it is extremely harmful to human health. The odor pollution has attracted more and more attention. Residents strongly opposed, petitioned, complained, and even the masses intercepted garbage trucks, Blocking field (factory) and other incidents, this problem has reached the point where it must be solved. Some have adopted many technical methods, such as physical deodorization, chemical deodorization, etc., but these technologies and methods, some of which consume a lot of energy, some...

Examples

Embodiment 1

[0023] A compound type high-efficiency microbial deodorant is prepared by the following method:

[0024] (1) Strain cultivation: Candida utilis, Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans and Lactobacillus faecalis were respectively inoculated in the sterilized medium for cultivation, and the components of the medium were: 1wt% of peptone, 1wt% of yeast extract, 2wt% of glucose, the balance is water, and the pH is 6.8. The bed speed is 175r / min, and the shaking table speed of bacillus coagulans and enterococcus faecalis is 20r / min. 8 cfu / ml), Bacillus subtilis bacteria liquid (the number of bacteria reaches 4.4×10 8 cfu / ml), Bacillus licheniformis bacteria liquid (the number of bacteria reaches 6×10 8 cfu / ml), Bacillus coagulans bacteria liquid (the number of bacteria reaches 2.9×10 8 cfu / ml), fecal intestinal lactic acid bacteria liquid (the number of bacteria reaches 6.4×10 9 cfu / ml);

[0025] (2) Bacteria solution compounding: according to the volume...

Embodiment 2

[0029] A compound type high-efficiency microbial deodorant is prepared by the following method:

[0030] (1) Strain cultivation: Candida utilis, Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans and Lactobacillus faecalis were respectively inoculated in the sterilized medium for cultivation, and the components of the medium were: 1wt% of peptone, 1wt% of yeast extract, 2wt% of glucose, the balance is water, and the pH is 7.0. The bed rotating speed is 170r / min, and the shaking table rotating speed of Bacillus coagulans and Enterococcus faecalis is 25r / min, and after the cultivation finishes, obtain the Candida utilis liquid (number of bacteria reaches 4.2 * 10 8 cfu / ml), Bacillus subtilis bacteria liquid (the number of bacteria reaches 4.7×10 8 cfu / ml), Bacillus licheniformis bacteria liquid (the number of bacteria reaches 6.3×10 8 cfu / ml), Bacillus coagulans bacteria liquid (the number of bacteria reaches 3.5×10 8 cfu / ml), fecal intestinal lactic acid bacteria ...

Embodiment 3

[0035] A compound type high-efficiency microbial deodorant is prepared by the following method:

[0036] (1) Strain cultivation: Candida utilis, Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans and Lactobacillus faecalis were respectively inoculated in the sterilized medium for cultivation, and the components of the medium were: Peptone 1wt%, yeast extract 1wt%, glucose 2wt%, the balance is water, pH is 7.0, the condition of culture is: temperature 31 ℃, time 13h, wherein the shaking of Candida utilis, Bacillus subtilis and Bacillus licheniformis The bed rotating speed is 150r / min, and the shaking table rotating speed of Bacillus coagulans and Enterococcus faecalis is 35r / min. 8 cfu / ml), Bacillus subtilis bacteria liquid (the number of bacteria reaches 5.7×10 8 cfu / ml), Bacillus licheniformis bacteria liquid (the number of bacteria reaches 6.7×10 8 cfu / ml), Bacillus coagulans bacteria liquid (the number of bacteria reaches 3.2×10 8 cfu / ml), fecal intestinal lac...

technical field

[0001] The invention belongs to the technical field of microbes, and in particular relates to a composite high-efficiency microbial deodorant and a preparation method thereof. Background technique

[0002] The stench emitted by garbage disposal sites and transfer stations, sewage treatment plants, livestock and poultry farms, etc. has become an important source of pollution in urban and rural environments. The pollution of the odor generated by the environment is increasing, and it is extremely harmful to human health. The odor pollution has attracted more and more attention. Residents strongly opposed, petitioned, complained, and even the masses intercepted garbage trucks, Blocking field (factory) and other incidents, this problem has reached the point where it must be solved. Some have adopted many technical methods, such as physical deodorization, chemical deodorization, etc., but these technologies and methods, some of which consume a lot of energy, some...

Examples

Embodiment 1

[0023] A compound type high-efficiency microbial deodorant is prepared by the following method:

[0024] (1) Strain cultivation: Candida utilis, Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans and Lactobacillus faecalis were respectively inoculated in the sterilized medium for cultivation, and the components of the medium were: 1wt% of peptone, 1wt% of yeast extract, 2wt% of glucose, the balance is water, and the pH is 6.8. The bed speed is 175r / min, and the shaking table speed of bacillus coagulans and enterococcus faecalis is 20r / min. 8 cfu / ml), Bacillus subtilis bacteria liquid (the number of bacteria reaches 4.4×10 8 cfu / ml), Bacillus licheniformis bacteria liquid (the number of bacteria reaches 6×10 8 cfu / ml), Bacillus coagulans bacteria liquid (the number of bacteria reaches 2.9×10 8 cfu / ml), fecal intestinal lactic acid bacteria liquid (the number of bacteria reaches 6.4×10 9 cfu / ml);

[0025] (2) Bacteria solution compounding: according to the volume...

Embodiment 2

[0029] A compound type high-efficiency microbial deodorant is prepared by the following method:

[0030] (1) Strain cultivation: Candida utilis, Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans and Lactobacillus faecalis were respectively inoculated in the sterilized medium for cultivation, and the components of the medium were: 1wt% of peptone, 1wt% of yeast extract, 2wt% of glucose, the balance is water, and the pH is 7.0. The bed rotating speed is 170r / min, and the shaking table rotating speed of Bacillus coagulans and Enterococcus faecalis is 25r / min, and after the cultivation finishes, obtain the Candida utilis liquid (number of bacteria reaches 4.2 * 10 8 cfu / ml), Bacillus subtilis bacteria liquid (the number of bacteria reaches 4.7×10 8 cfu / ml), Bacillus licheniformis bacteria liquid (the number of bacteria reaches 6.3×10 8 cfu / ml), Bacillus coagulans bacteria liquid (the number of bacteria reaches 3.5×10 8 cfu / ml), fecal intestinal lactic acid bacteria ...

Embodiment 3

[0035] A compound type high-efficiency microbial deodorant is prepared by the following method:

[0036] (1) Strain cultivation: Candida utilis, Bacillus subtilis, Bacillus licheniformis, Bacillus coagulans and Lactobacillus faecalis were respectively inoculated in the sterilized medium for cultivation, and the components of the medium were: Peptone 1wt%, yeast extract 1wt%, glucose 2wt%, the balance is water, pH is 7.0, the condition of culture is: temperature 31 ℃, time 13h, wherein the shaking of Candida utilis, Bacillus subtilis and Bacillus licheniformis The bed rotating speed is 150r / min, and the shaking table rotating speed of Bacillus coagulans and Enterococcus faecalis is 35r / min. 8 cfu / ml), Bacillus subtilis bacteria liquid (the number of bacteria reaches 5.7×10 8 cfu / ml), Bacillus licheniformis bacteria liquid (the number of bacteria reaches 6.7×10 8 cfu / ml), Bacillus coagulans bacteria liquid (the number of bacteria reaches 3.2×10 8 cfu / ml), fecal intestinal lac...

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