A kind of preparation method and application of carotenoid 9, 10' dioxygenase

A carotene and dioxygenase technology, applied in the field of bioengineering, can solve the problems of low yield, high requirement of reaction conditions, long reaction time of β-ionone, etc., and achieves short reaction time, less reaction products and optimized Effects of reaction conditions
CN106282137BActive Publication Date: 2019-09-13ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
Publication Date
2019-09-13

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Abstract

The invention belongs to the field of bioengineering and relates to a preparation method and application of a carotenoid 9,10' dioxygenase. The preparation method comprises the following steps: cultivating enterobacter hormaechei, and extracting a whole genome sequence; constructing a recombinant plasmid of a carotenoid 9,10' dioxygenase gene; inducing expression of the carotenoid 9,10'dioxygenase gene, and purifying zymoprotein; and analyzing and identifying enzymatic properties of the carotenoid 9,10' dioxygenase. The prepared carotenoid 9,10' dioxygenase gene can efficiently catalyze and synthesize beta-ionone, catalysis specificity is high, reaction products are few, and reaction time is short; a novel enzyme resource different from discovered carotenoid 9,10' dioxygenase is obtained; the carotenoid 9,10' dioxygenase has different amino acid sequences and enzymatic characteristics; and reaction conditions are optimized, and yield of the beta-ionone reaches 79.4%.
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Description

technical field

[0001] The invention belongs to the field of bioengineering, and relates to a preparation method and application of carotenoid 9, 10' dioxygenase. Background technique

[0002] β-ionone is a natural fragrance and an important intermediate, widely used in food, cosmetics and pharmaceutical industries. As a representative of cyclized isoprenoids, β-ionone has a wide range of biological activities, especially exhibiting good antitumor activity. As a plant secondary metabolite, β-ionone is extracted from plant essential oils in a small amount and is expensive, and currently it is mainly synthesized by chemical methods. The chemical method has high requirements on reaction conditions and separation equipment, the product is not easy to separate, and the waste liquid produced pollutes the environment. In addition, with the improvement of living standards and the enhancement of health awareness, the safety of chemically synthesized β-ionone has been questioned mor...

Claims

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