Method for detecting uric acid with G-MoS2-Nafion

Abstract

The invention discloses a method for detecting uric acid with G-MoS2-Nafion. The method is characterized by including the following steps that firstly, a G-MoS2-Nafion mixture is prepared; secondly, 0.005 ml of G-MoS2-Nafion compound ethyl alcohol suspension is sucked and dropwise added to the surface of a glassy carbon electrode, the glassy carbon electrode is placed at the room temperature to be naturally dried for 30 min, and the glassy carbon electrode modified with G-MoS2-Nafion is obtained; thirdly, the glassy carbon electrode is put into a phosphate buffer solution with the PH of 7.4 to be stabilized for 2 h; fourthly, separated plasma is added into the PBS to be diluted right by 5 folds, stirring is carried out for 20 s, the current value of diluted plasma is measured with the chronoamperometry according to the three-electrode system measurement principle, and the concentration value of uric acid in detected plasma is obtained through conversion.

Description

technical field [0001] The present invention relates to the field of quantitative detection of electrochemical substances, in particular to a method for detecting uric acid by using graphene-molybdenum disulfide-perfluorosulfonic acid resin. Background technique [0002] Modern medicine has proved that uric acid (UA) is not only an end product in the process of purine metabolism in the human body, the existence of UA plays an important role in human metabolism, such as transmitting nerve signals as a neurotransmitter. On the other hand, when the UA level in the human body is abnormal, it has important reference value for the diagnosis, treatment, monitoring and prevention of certain diseases, such as gout, hyperuricemia, renal failure, urinary tract stones, hypertension, coronary heart disease, leukemia , arthritis, Lesch-Nyhan syndrome, schizophrenia, and Parkinson's. [0003] At present, the methods for UA detection of uric acid include enzymatic method, spectrophotometri...

AI Technical Summary

Problems solved by technology

1). The enzymatic method uses the unique molecular recognition ability and specific catalytic effect of uricase, which can well eliminate the interference of other substances, but its disadvantage is that it is expensive; 2). The advantage of high performance liquid chromatography is the separation effect It is better, faster, and the mobile phase used is simple, but the sample pretreatment steps are cumbersome and time-consuming, and there are many inconveniences in the detection; 3). Based on the principle of complex, the blood uric acid content is judged by light colorimetry. This method has good accuracy and simple operation, but its disadvantages are poor sensitivity and specificity, and complicated pretreatment of samples, which limits its scope of application.

In short, the above UA detection methods have major defects such as low sensitivity, complicated detection process, narrow linear range, and high detection cost.

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