Solid Phase Extraction-Gas Chromatography-Tandem Mass Spectrometry Detection Method of Amide Fungicides in Wine

Abstract

The invention discloses a solid phase extraction-gas chromatography-tandem mass spectrum detection method for amide fungicides in wine. The method can detect four types amide fungicides in a wine sample at the same time, including indazole sulfonyl fluopicolide, fluopicolide, silthiopham, and boscalid. The solid phase extraction-gas chromatography-tandem mass spectrum detection method includes the following steps: diluting the wine sample with ultra pure water to obtain the liquid to be detected, purifying the liquid to be detected with HLB solid phase extraction column, and separating the liquid to be detected with C18 reversed-phase column, performing the qualitative analysis on a target analyte in the multiple reaction monitoring mode, based on the comparison between retention time and ion pair information, and quantifying the target analyte with an isotope internal standard method. The method has fast analysis speed, high sensitivity, good reproducibility and good accuracy, and is a new detection method for the evaluation of the quality of the wine sample.

Description

technical field [0001] The invention belongs to the field of food detection, relates to solid phase extraction-gas chromatography-tandem mass spectrometry (GC-MS / MS), specifically refers to a kind of indazole sulfame, fluopyram, silthiocarb, The detection method of 4 kinds of amide fungicides such as boscalid. Background technique [0002] With the improvement of people's living standards and the enhancement of health awareness, wine, a special drink that integrates nutrition, functionality and cultural products, is more and more popular among people. As a relatively developed province on the southeast coast of my country, Zhejiang's wine consumption has grown rapidly in recent years, especially imported wine. The trade volume of imported wine at Zhejiang Port has always been in the leading position in the same industry in the country. In 2015, Zhejiang ports imported a total of 31,100 tons of wine, an increase of 10.2% over the previous year, with a value of 120 million U...

AI Technical Summary

Problems solved by technology

[0003] However, no matter whether it is imported or domestic wine, grapes are the most important raw material in the field management process. In order to prevent the occurrence of diseases, a certain amount of pesticides are usually sprayed. Some pesticides are not easy to remove and decompose during the fermentation process of wine production. Residues in wine to varying degrees, posing a potential threat to the health of drinkers

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