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A kind of biological enzymatic exfoliating facial mask making and using method

A technology of biological enzyme method and production method, which is applied in the direction of biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of leading enterprises without representative significance, and achieve high safety effects

Inactive Publication Date: 2019-04-05
TAISHAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current facial mask market, there is no domestic production and sales of facial masks that use purified keratinase biological exfoliation. Most of the biological enzyme exfoliating masks are still in the experimental stage, and there are no representative leading companies.

Method used

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Experimental program
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Embodiment Construction

[0019] The present invention is further described in detail below.

[0020] A method for making and using a bio-enzymatic exfoliating facial mask, characterized in that:

[0021] The casein material is pulverized, sterilized by high-pressure steam for 30 minutes, and then baked in an oven at 80°C for 12 hours to constant weight;

[0022] Keratinase-producing bacteria were inoculated in 100ml of LB medium (Luria-Bertani medium) at 1% inoculum, 160 rpm / min (rotation speed / minute), and activated by shaking for 24 hours; then inoculated at 2% inoculum in 150ml LB (Luria-Bertani medium) culture medium, 160 rpm, about 3~4 hours, shaking culture to logarithmic phase (OD6 00 0.4 ~ 0.6, OD: optical density) to obtain keratinase-producing bacterial inoculum;

[0023] 5 L fermenter to produce keratinase, liquid volume 3.5 L, 52.5 g of materials rich in keratinized protein such as pre-treated feathers, hair, keratinized skin or casein, K 2 HPO 4 4.9 g, KH 2 PO 4 2.45 g, NaCl 1.75...

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Abstract

The invention discloses a method for making and using a biological enzymatic exfoliating facial mask. The invention pretreats the raw material of the biological enzymatic mask, prepares keratinase, optimizes enzyme action conditions, combines keratinase with cotton linter material mask, and keratinase Cryopreservation and mask room temperature are achieved using a series of steps. The present invention makes full use of keratin-degrading bacteria—Stenophora maltophilia ( Microbacterium sp .TSMC‑99) is fermented to produce keratinase. The keratinase adsorbed on the mask catalyzes the degradation of part of the keratinized tissue of the skin, removes keratinized dandruff on the face, removes dead skin, moisturizes the face, makes the skin clear and bright, and plays a role Skin rejuvenation. Bio-enzymatic exfoliating mask is a non-toxic, non-polluting, non-side effect, high-safety bio-tech product. Moreover, the production conditions are mild, environmentally friendly, easy to operate, easy to promote, and low in cost. The technical indicators of the manufactured mask meet the specifications of the light industry standard "QB / T 2872‑2007 Mask".

Description

technical field [0001] The invention relates to a method for making and using a bio-enzymatic exfoliating facial mask. Background technique [0002] With the progress of the times and the development of the economy, more and more people begin to pay attention to the maintenance of personal skin quality. Among them, facial masks have been recognized by more and more people as cosmetics for cleaning, caring and nourishing the facial skin. Facial mask has become the fastest-growing market segment in my country's daily chemical industry. It ranked third in the cosmetics market in 2013 and 2014, accounting for 16.3% of the total market sales. In 2015, it reached 30 billion yuan, and the future market space will reach 200 billion yuan. Yuan. According to the latest report released by Kantar Worldpanel, facial masks contributed 41% of the sales increase to the skin care market in 2015. In 2015, 48% of urban households in China bought facial masks, and its development has surpassed...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/66A61K8/64A61K8/02A61Q19/00C12N9/52C12R1/01
CPCA61K8/0212A61K8/64A61K8/66A61K2800/85A61Q19/00C12N9/52
Inventor 史仁玖张显忠李艳玲
Owner TAISHAN MEDICAL UNIV
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