Application of CdTe QD@ZIF-8 core-shell nano composite material in detection of oxidase activity

Abstract

The invention discloses application of a CdTe QD@ZIF-8 core-shell nano composite material in detection of oxidase activity, the CdTe QD@ZIF-8 core-shell nano composite material has the advantages of size selection, good stability and good dispersibility of solution, and the synthetic method has simplicity, mild conditions and short time. According to the application of the CdTe QD@ZIF-8 core-shell nano composite material in detection of oxidase activity, the reaction product H2O2 of oxidase with a corresponding substrate can penetrate through a material shell to react with CdTe QD, other molecules greater than the pore diameter of the shell are obstructed, so that difference in fluorescence signals is generated, and the content of oxidase in a system can be detected by fluorescence spectrophotometry; the operation is simple, and the used detection reagent greatly reduces the interference of background fluorescence to the detected system, so that the detection precision is ensured; and the application of the CdTe QD@ZIF-8 core-shell nano composite material has the advantages of high sensitivity, strong antijamming capability, good stability and convenience in use and operation on a fully automatic biochemical analysis instrument as compared with double reagents.

Description

technical field [0001] The invention belongs to the technical field of clinical in vitro detection, and specifically relates to the application of a CdTe QD@ZIF-8 core-shell nanocomposite material in the detection of oxidase activity. Background technique [0002] Quantum dots (QDs) have attracted extensive attention in many research fields due to their unique photophysical and chemical properties, such as broad absorption band, narrow and symmetric emission band, low photobleaching, long lifetime, high quantum yield, etc., including Physical, chemical, biological sciences. However, from the perspective of long-term observation, the fluorescence of quantum dots is highly susceptible to the influence of biomolecules in the microenvironment, such as proteins, enzymes, and cell metabolites. This phenomenon is often affected by the number of surface defects, and the intrinsically imperfect crystal structure can directly lead to fluorescence quenching of QDs. A second-layer pas...

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Problems solved by technology

The NPs@MOF core-shell nanocomposites reported above mainly focus on heterogeneous catalysis, and there are not many reports on the application in biosensors, because there are major challenges in the application in biological systems, such as stability, dispersibility, toxicity, biological Compatibility, etc. (Sharifi, S, et al., 2012. Chem. Soc. Rev. 41, 2323-2343; Baati, T. et al., 2013. Chem. Sci. 4, 1597-1607)

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