Application of CdTe QD@ZIF-8 core-shell nano composite material in detection of oxidase activity
A technology of nanocomposite materials and oxidase activity, which is applied in the direction of material analysis, material excitation analysis, material analysis through optical means, etc., to achieve the effect of easy operation, accuracy and short time
Inactive Publication Date: 2017-02-01
SHAANXI NORMAL UNIV
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Problems solved by technology
The NPs@MOF core-shell nanocomposites reported above mainly focus on heterogeneous catalysis, and there are not many reports on the application in biosensors, because there are major challenges in the application in biological systems, such as stability, dispersibility, toxicity, biological Compatibility, etc. (Sharifi, S, et al., 2012. Chem. Soc. Rev. 41, 2323-2343; Baati, T. et al., 2013. Chem. Sci. 4, 1597-1607)
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Embodiment 1
[0022] Dissolve 84 mg (1 mmol) 2-methylimidazole completely in 3 mL ultrapure water, then add 500 μL CdTe QD@PVP (prepared according to the method disclosed by Lu, G. et. al. 2012. Nat. Chem. 4, 310-316) , stirred at room temperature for 5 minutes, then added 3mL Zn(NO 3 ) 2 ·6H 2 O aqueous solution (containing 76mg (0.25mmol) Zn(NO 3 ) 2 ·6H 2 O), continue to stir for 40 minutes, centrifuge, wash with ultrapure water for 3 to 5 times, and dry in vacuum at 65°C overnight to obtain CdTe QD@ZIF-8 core-shell nanocomposites.
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The invention discloses application of a CdTe QD@ZIF-8 core-shell nano composite material in detection of oxidase activity, the CdTe QD@ZIF-8 core-shell nano composite material has the advantages of size selection, good stability and good dispersibility of solution, and the synthetic method has simplicity, mild conditions and short time. According to the application of the CdTe QD@ZIF-8 core-shell nano composite material in detection of oxidase activity, the reaction product H2O2 of oxidase with a corresponding substrate can penetrate through a material shell to react with CdTe QD, other molecules greater than the pore diameter of the shell are obstructed, so that difference in fluorescence signals is generated, and the content of oxidase in a system can be detected by fluorescence spectrophotometry; the operation is simple, and the used detection reagent greatly reduces the interference of background fluorescence to the detected system, so that the detection precision is ensured; and the application of the CdTe QD@ZIF-8 core-shell nano composite material has the advantages of high sensitivity, strong antijamming capability, good stability and convenience in use and operation on a fully automatic biochemical analysis instrument as compared with double reagents.
Description
technical field [0001] The invention belongs to the technical field of clinical in vitro detection, and specifically relates to the application of a CdTe QD@ZIF-8 core-shell nanocomposite material in the detection of oxidase activity. Background technique [0002] Quantum dots (QDs) have attracted extensive attention in many research fields due to their unique photophysical and chemical properties, such as broad absorption band, narrow and symmetric emission band, low photobleaching, long lifetime, high quantum yield, etc., including Physical, chemical, biological sciences. However, from the perspective of long-term observation, the fluorescence of quantum dots is highly susceptible to the influence of biomolecules in the microenvironment, such as proteins, enzymes, and cell metabolites. This phenomenon is often affected by the number of surface defects, and the intrinsically imperfect crystal structure can directly lead to fluorescence quenching of QDs. A second-layer pas...
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IPC IPC(8): G01N21/64
CPCG01N21/64G01N21/6428G01N2021/6432
Inventor 党福全王科李南海小满刘俊霞杨兰芳
Owner SHAANXI NORMAL UNIV
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