Method for extracting beta-D-glucan from cell walls of candida utilis

A Candida utilis and cell wall technology, applied in the field of biology, can solve the problems of product yield and biological activity reduction, severe acid-base extraction conditions, low product purity, etc., to protect gastric mucosa, lower blood sugar, immunity, The effect of reducing pollution

Inactive Publication Date: 2017-02-15
SHANGHAI INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the domestic methods of extracting β-D-glucan mainly use acid method, alkali method, and acid-base combination. - Dextran will degrade, resulting in a decrease in product yield and biological activity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of β-D-glucan of Candida utilis cell wall is prepared by the method comprising the following steps:

[0023] (1) Acquisition of Candida utilis cells

[0024] After culturing Candida utilis with protein-free potato juice for 14 hours, take 50 mL of protein-free potato juice culture solution, centrifuge (15500g / 4°C / 10min, refrigerated centrifuge), and then wash twice with deionized water to obtain the product For Candida prion cells, the cell pellet can be stored in the refrigerator (4°C);

[0025] The used Candida utilis (Candidautilis), deposit number CICC1769;

[0026] (2) Induced autolysis of cells

[0027] The Candida utilis cells obtained in step (1) are induced to autolyse, that is, lysosomes release enzymes to degrade their own cells and let the contents flow out. Yeast was autolyzed at a concentration of 5% (w / w), pH 7.0, 5% NaCl and 60°C, shaking at 120rpm. Under this condition, the time for yeast cells to induce autolysis is 24 hours. Centrifuge aft...

Embodiment 2

[0040] A kind of β-D-glucan of Candida utilis cell wall is prepared by the method comprising the following steps:

[0041] (1) Acquisition of Candida utilis cells

[0042] Cultivate Candida utilis with protein-free potato juice for 10 hours, take 50 mL of protein-free potato juice culture medium, centrifuge (15500g / 4°C / 10min, refrigerated centrifuge), and then wash twice with deionized water to obtain urinogen For Candida cells, the cell pellet can be stored in the refrigerator (4°C);

[0043]The used Candida utilis (Candidautilis), deposit number CICC1769;

[0044] (2) Induced autolysis of cells

[0045] The Candida utilis cells obtained in step (1) are induced to autolyse, that is, lysosomes release enzymes to degrade their own cells and let the contents flow out. Yeast was autolyzed at a concentration of 10% (w / w), pH 4.0, 2% NaCl and 55°C, shaking at 100 rpm. Under this condition, the time for yeast cells to induce autolysis is 20 hours. Centrifuge after autolysis, an...

Embodiment 3

[0058] A kind of β-D-glucan of Candida utilis cell wall is prepared by the method comprising the following steps:

[0059] (1) Acquisition of Candida utilis cells

[0060] Cultivate Candida utilis with deproteinized potato juice for 12 hours, take 50 mL of the culture medium of deproteinized potato juice, centrifuge (15500g / 4°C / 10min, refrigerated centrifuge), and then wash twice with deionized water to obtain urinogen For Candida cells, the cell pellet can be stored in the refrigerator (4°C);

[0061] The used Candida utilis (Candidautilis), deposit number CICC1769;

[0062] (2) Induced autolysis of cells

[0063] The Candida utilis cells obtained in step (1) are induced to autolyse, that is, lysosomes release enzymes to degrade their own cells and let the contents flow out. Yeast was autolyzed at a concentration of 15% (w / w), pH 5.0, 3% NaCl and 56°C, shaking at 110rpm. Under this condition, the time for yeast cells to induce autolysis is 21 hours. Centrifuge after auto...

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Abstract

The invention provides a method for extracting beta-D-glucan from cell walls of candida utilis. The method includes steps of acquiring cells of the candida utilis; carrying out induction autolysis on the cells of the candida utilis; carrying out high-temperature digestion; ultrasonically breaking the walls; carrying out high-temperature extraction; carrying out degreasing; carrying out enzymatic hydrolysis to remove proteins, to be more specific, preparing suspension from degreased crude glucan by the aid of phosphoric acid buffer solution, carrying out enzymatic hydrolysis, carrying out reaction, then washing reaction products by the aid of water, centrifuging the reaction products, adding water into the reaction products, sufficiently uniformly mixing the reaction products and the water with one another to obtain mixtures, then heating the mixtures, deactivating enzymes, centrifuging the mixtures and freezing and drying the mixtures to obtain beta-D-glucan products. The method has the advantages that product degradation effects due to the traditional methods implemented by the aid of acid and alkali reagents can be prevented, and the method for extracting beta-glucan can be carried out under mild conditions; potato juice without the proteins is used as a culture medium for the candida utilis, accordingly, environmental pollution due to the discarded potato juice can be abated, and the method has important significance on environmental protection.

Description

technical field [0001] The invention belongs to the field of biology and relates to a β-D-glucan, specifically a method for extracting the β-D-glucan from the cell wall of Candida utilis. Background technique [0002] Candida utilis is not pathogenic, and together with brewer's yeast and Kluyveromyces, it has been certified as a microorganism that can be used as a food additive in many countries, and it is also a microorganism that can be used in health food that is allowed by the Chinese health department. Yeast cell walls are generally used directly as feed products, so the added value is too low. [0003] Yeast beta-glucan is a structural polysaccharide of the yeast cell wall. They have different functional properties, such as medical, pharmaceutical, food, cosmetic and other industries. They can also act as biological modifiers of the immune response. In addition, they are also beneficial to antibacterial and anti-inflammatory reactions; promote the absorption of myco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
CPCC08B37/0024C08B37/0003
Inventor 马霞刘蓝天
Owner SHANGHAI INST OF TECH
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