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Method for increasing the yield of acetoin by using mixed bacteria fermentation

A technology of mixed bacteria fermentation and acetoin, which is applied in the field of fermentation engineering, can solve the problems of long experimental period, complicated coenzyme regeneration system itself, increased fermentation cost, etc., and achieves the effect of increasing yield

Inactive Publication Date: 2019-12-03
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity of constructing the coenzyme regeneration system itself, the long experiment period and the increase of fermentation costs due to the addition of exogenous sources, there is an urgent need for a method to increase the production of acetoin

Method used

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  • Method for increasing the yield of acetoin by using mixed bacteria fermentation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for increasing the yield of acetoin by using mixed bacteria fermentation includes the following steps:

[0018] In the experimental group, EC-Rib 02 seeds were inserted into the fermentation medium at an inoculum density of 0.05 in biomass, fermented at 32°C and a stirring speed of 100 rpm for 3 hours, connected to CJX-518, and continued fermentation; EC-Rib 02 and The biomass ratio of CJX-518 is 1:1, and fermentation is continued, and the total time from connecting CJX-518 to the end of fermentation is 72 hours to obtain the fermentation broth containing acetoin.

[0019] In the blank group, CJX-518 seeds were inserted into the fermentation medium at an inoculum density with a biomass of 0.05, and fermented at 32°C and a stirring speed of 100 rpm. The total fermentation time was 72 hours to obtain a fermentation broth containing acetoin. .

[0020] The fermentation medium of the experimental group and the blank group was formulated according to g / L: glucose 30, yeas...

Embodiment 2

[0023] A method for increasing the yield of acetoin by using mixed bacteria fermentation includes the following steps:

[0024] In the experimental group, EC-Rib 02 seeds were inserted into the fermentation medium at an inoculum density of 0.2 in biomass, fermented for 4 hours at 37°C and a stirring speed of 200 rpm, connected to CJX-518, and continued fermentation; EC-Rib 02 and The biomass ratio of CJX-518 is 2:1, and fermentation is continued, and the total time from connecting CJX-518 to the end of fermentation is 72 hours to obtain a fermentation broth containing acetoin.

[0025] In the blank group, CJX-518 seeds were inserted into the fermentation medium at an inoculum density of 0.1 biomass, and fermented at 37°C and a stirring speed of 200 rpm. The total fermentation time was 72 hours to obtain a fermentation broth containing acetoin. .

[0026] The fermentation medium of the experimental group and the blank group was formulated according to g / L: glucose 40, yeast powder 1,...

Embodiment 3

[0030] A method for increasing the yield of acetoin by using mixed bacteria fermentation includes the following steps:

[0031] Insert EC-Rib 02 seeds into the fermentation medium at an inoculum density of 0.8 biomass, ferment for 5 hours at 42°C and 300 rpm stirring speed, connect to CJX-518, and continue fermentation; EC-Rib 02 and CJX-518 The biomass ratio is 4:1, continue the fermentation, the total time from connecting CJX-518 to the end of the fermentation is 72 hours, and the fermentation broth containing acetoin is obtained.

[0032] In the blank group, CJX-518 seeds were inserted into the fermentation medium at an inoculum density of 0.2 in biomass, and fermented at 42°C and a stirring speed of 300 rpm. The total fermentation time was 72 hours to obtain a fermentation broth containing acetoin. .

[0033] The fermentation medium of the experimental group and the blank group was formulated according to g / L: glucose 50, yeast powder 1, peptone 10, NaCl 5, K 2 HPO 4 1, KCl 0.5...

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Abstract

The invention discloses a method for increasing acetoin yield through mixed fermentation. The method comprises the following steps: a fermentation medium is inoculated with seeds of escherichia coli engineering strains EC-Rib02 of high-yield riboflavin at the inoculation density adopting biomass being 0.05-0.8, the seeds are fermented for 3-5 h under the conditions that the temperature is 32-42 DEG C and the stirring rotating speed is 100-300 rpm, then Paenibacillus polymyxa CJX-518 with the preservation number of CGMCC NO.7096 is inoculated with the seeds, and the seeds are fermented continuously, wherein the biomass ratio of the escherichia coli engineering strains EC-Rib02 of the high-yield riboflavin to the Paenibacillus polymyxa CJX-518 with the preservation number of CGMCC NO.7096 is (1-4):1. The method can increase the acetoin yield.

Description

Technical field [0001] The invention belongs to the technical field of fermentation engineering, and relates to a method for improving the yield of acetoin by using mixed bacteria fermentation. Background technique [0002] Acetoin is a flavor additive, which can be widely used in the pharmaceutical and chemical industries. Paenibacillus polymyxa (Paenibacillus polymyxa) CJX-518 with the deposit number CGMCC NO.7096 mainly produces 2,3-butanediol in the process of biological metabolism, and also produces a small amount of acetoin. In order to increase the production of acetoin, On the one hand, researchers introduced a cofactor regeneration system through coenzyme engineering to adjust the distribution ratio of acetoin and 2,3-butanediol. On the other hand, the extracellular redox state and the intracellular NADH / NAD + Closely related, researchers add oxidizing or reducing agents (NaBH 4 , DTT, Vc) to achieve NADH / NAD + The adjustment of acetoin and 2,3-butanediol distribution r...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P7/26C12R1/01C12R1/19
CPCC12P7/26C12P39/00
Inventor 程景胜刘蕾元英进陈涛赵学明
Owner TIANJIN UNIV