Unlock instant, AI-driven research and patent intelligence for your innovation.

A Hierarchical Screening Model Based on Time Parameters and Cleavage Patterns

A technology of time parameters and models, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of lack of effective indicators, unable to fully reflect embryo development potential, etc., to reduce multiple pregnancies and effectively evaluate embryos. Effect

Active Publication Date: 2021-03-26
THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the evaluation of embryos based only on the above unilateral indicators has certain limitations, cannot fully reflect the developmental potential of embryos, and lacks recognized effective indicators

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A Hierarchical Screening Model Based on Time Parameters and Cleavage Patterns
  • A Hierarchical Screening Model Based on Time Parameters and Cleavage Patterns

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Embryo culture and dynamic observation of 1728 embryos from 211 IVF cycles were carried out by TLI, and embryo evaluation was performed by applying a hierarchical screening model:

[0022] For ovulation induction by conventional ovulation induction scheme, the obtained oocytes were fertilized in IVF culture medium (Vitrolife, Sweden), and the fertilized eggs were transferred to WOW dishes containing G1 sequential culture medium (Vitrolife, Sweden), and placed in Primo Cultured in TLI (37°C / 6% CO 2 ) and observation. The system is a real-time embryo monitoring tool, which consists of a digital inverted embryo microscope, a console host, a special software system and a special collection culture dish (WOW dish, Cryo-Innovation). The digital inverted embryo microscope is placed in the traditional incubator, and the console host is placed outside the incubator, connected to the microscope in the incubator through a USB data cable, and the frequency of taking pictures ...

Embodiment 2

[0030] The patients were randomly selected into the group and divided into the TLI observation group and the traditional morphological evaluation group (there was no statistical difference in the basic conditions of the two groups, see Table 1), and single embryo transfer at the cleavage stage was carried out on the third day.

[0031] Table 1 General information of patients with single embryo transfer

[0032] TLI observation group Traditional Morphological Group Number of cycles (n) 88 56 age (years) 33.53±3.59 32.57±3.62 BMI (kg / m 2 )

23.69±3.82 24.59±3.71 Basal FSH (mIU / ml) 7.80±1.83 7.28±2.06 Basal LH(m IU / ml) 4.03±2.36 4.17±2.57 Number of retrieved eggs (n) 11.50±2.99 11.41±3.02 Normal fertilization number (n) 8.44±2.77 8.46±2.74 Normal cleavage number (n) 8.19±2.80 8.23±2.88

[0033] Eighty-eight patients entered the TLI observation group. After routine ovulation induction, the fertilized eggs ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to an embryo quality evaluation method based on time parameter and cleavage mode combination. The embryo quality evaluation method comprises: 1) carrying out embryo culture and dynamic observation on fertilized eggs through a TLI system; 2) selecting the embryos according to a cleavage mode, and placing the embryos having the normal cleavage, the non-uniform embryo or the non-axial cleavage into the next layer screening; and 3) selecting according to time parameters, wherein s2 and t5 are adopted as the optimal high-quality embryo selection time parameters to perform re-layering screening, and the time parameters of the embryo meet that s2 is less than or equal to 0.50 h or / and t5 is less than or equal to 48.93 h. With the embryo quality evaluation method of the present invention, the limitations of the traditional morphological evaluation and the current TLI embryo evaluation method are overcome, the embryo development potential can be conveniently predicted, the high quality embryo can be efficiently and rapidly screened, and the pregnancy outcome can be ensured.

Description

technical field [0001] The invention relates to a method for evaluating embryos of patients undergoing in vitro fertilization-embryo transplantation and selecting high-quality embryos for transplantation in assisted reproductive technology. Background technique [0002] Multiple pregnancy is considered to be one of the most common and serious complications in assisted reproductive technology (Assisted Reproductive Technology, ART). In order to avoid multiple pregnancy, the implementation of single embryo transfer (Single EmbryoTransfer, SET) as an important measure of prenatal and postnatal care has become the main direction of assisted reproduction. The core link to promote the development of SET is to accurately evaluate the developmental potential of embryos and select the best embryos for transplantation. [0003] Among the many assessment methods, morphological assessment is the most commonly used method for clinical embryo quality assessment. It is based on the evalu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02
Inventor 王珊珊招霞孙海翔
Owner THE AFFILIATED DRUM TOWER HOSPITAL MEDICAL SCHOOL OF NANJING UNIV