A kind of bacterial agent for treating soil heavy metal pollution and its preparation method and application

A heavy metal and soil technology, applied in the field of soil remediation, can solve the problems of lack of soil remediation technology, limit the scope of application, affect the ecological environment, etc., and achieve the effects of controlling heavy metal pollution, increasing the scope of application and simple preparation process

Active Publication Date: 2019-03-08
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dumps, tailings ponds and surrounding polluted sites formed by mining activities often have complex pollutant components, containing heavy metals and various organic pollutants. These heavy metals have high pollution concentrations and serious pollution levels, making it difficult for animals and plants to grow in them , seriously affecting the ecological environment
Heavy metals in the soil can pollute the surrounding land, waters and atmosphere through surface water runoff and atmospheric dust, and its impact will far exceed the area and space of the waste dump. The impact of pollution will cost a lot of manpower, material resources and financial resources It takes a long time to recover, and it is difficult to return to the original level
[0003] Modern technology to deal with heavy metal pollution soil remediation technology is relatively scarce, mainly focusing on the enrichment of specific heavy metals by hyperaccumulative plants. This method can only deal with relatively single heavy metal pollution, and plants have high requirements on the soil environment, which limits its application. scope

Method used

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  • A kind of bacterial agent for treating soil heavy metal pollution and its preparation method and application
  • A kind of bacterial agent for treating soil heavy metal pollution and its preparation method and application
  • A kind of bacterial agent for treating soil heavy metal pollution and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A microbial bacterial agent for treating heavy metal pollution in soil consists of the following raw materials in parts by weight:

[0045] 80 parts of rice husk, 20 parts of pig manure, 50 parts of chicken manure, 30 parts of soybean powder, 6 parts of single-cell protein, 2.5 parts of Mortierella alpina fermentation broth, 2.5 parts of Trichoderma asperellum fermentation broth part, 0.5 part of Mucor circinelloides fermentation broth;

[0046] The total content of bacteria in the microbial agent is 5.3×10 8 cfu / g; the ratio of the number of live bacteria of Mortierella alpina, Trichoderma asperellum and Mucor circinelloides is 1:1.8:2.5.

[0047] The preparation method of the microbial inoculant for the above-mentioned treatment of soil heavy metal pollution, the steps are as follows:

[0048] (1) Mortierella alpina (Mortierella alpina) was cultivated through primary seed cultivation, secondary seed cultivation and expansion cultivation to produce 0.4×10 8 The Mort...

Embodiment 2

[0060] A microbial bacterial agent for treating heavy metal pollution in soil consists of the following raw materials in parts by weight:

[0061] 50 parts of rice husk, 50 parts of pig manure, 20 parts of chicken manure, 60 parts of soybean powder, 4 parts of single cell protein, 3.5 parts of Mortierella alpina fermentation broth, 1.5 parts of Trichoderma asperellum fermentation broth Parts, 1.5 parts of Mucor circinelloides fermentation broth;

[0062] The total content of bacteria in the microbial agent is 8.3×10 8 cfu / g; the ratio of the number of live bacteria of Mortierella alpina, Trichoderma asperellum and Mucor circinelloides is 1:1:3.6.

[0063] The preparation method is the same as in Example 1.

Embodiment 3

[0065] A microbial bacterial agent for treating heavy metal pollution in soil consists of the following raw materials in parts by weight:

[0066] 65 parts of rice husk, 35 parts of pig manure, 35 parts of chicken manure, 45 parts of soybean powder, 5 parts of single-cell protein, 3 parts of Mortierella alpina fermentation broth, 2 parts of Trichoderma asperellum fermentation broth 1 part, 1 part of Mucor circinelloides fermentation liquid;

[0067] The total content of bacteria in the microbial agent is 6.8×10 8 cfu / g; the ratio of the number of live bacteria of Mortierella alpina, Trichoderma asperellum and Mucor circinelloides is 1:1.5:3.

[0068] The preparation method is the same as in Example 1.

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Abstract

The present invention relates to a microbial agent for treating heavy metal polluted soil, a preparation method and applications thereof. The microbial agent comprises the following raw materials: rice husk, pig manure, chicken manure, soybean powder, single cell protein, a Mortierella alpina fermentation broth, a Trichoderma asperellum fermentation broth, and a Mucor circinelloides fermentation broth. According to the present invention, the microbial agent prepared by mixing Mortierella alpina, Trichoderma asperellum and Mucor circinelloides according to a certain ratio is firstly used, can be used in the complex and changing environment, and can provide effects in the poor soil environment, wherein the functional microbes provide high tolerance to heavy metals, and can maintain activity and perform efficient repair in the heavily heavy metal polluted soil.

Description

technical field [0001] The invention relates to a bacterial agent for treating heavy metal pollution in soil, a preparation method and application thereof, and belongs to the technical field of soil treatment. Background technique [0002] Ecological damage has become a key issue restricting human development. The dumps, tailings ponds and surrounding polluted sites formed by mining activities often have complex pollutant components, containing heavy metals and various organic pollutants. These heavy metals have high pollution concentrations and serious pollution levels, making it difficult for animals and plants to grow in them , seriously affecting the ecological environment. Heavy metals in the soil can pollute the surrounding land, waters and atmosphere through surface water runoff and atmospheric dust, and its impact will far exceed the area and space of the waste dump. The impact of pollution will cost a lot of manpower, material resources and financial resources It ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10C12N1/14C12R1/885C12R1/785C12R1/645
CPCB09C1/105C12N1/14
Inventor 张旭杨欢欢崔兆杰孙立群张萌萌魏绍山
Owner SHANDONG UNIV
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