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Construction and application of adenosine 5'-monophosphate mediated nano transmission system for target tumor

A monophosphoric acid, tumor technology, applied in the field of biomedical engineering, can solve problems such as insufficient realization

Active Publication Date: 2017-03-08
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, despite some penetration and retention effects, it is still insufficient for very specific targeting

Method used

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  • Construction and application of adenosine 5'-monophosphate mediated nano transmission system for target tumor
  • Construction and application of adenosine 5'-monophosphate mediated nano transmission system for target tumor
  • Construction and application of adenosine 5'-monophosphate mediated nano transmission system for target tumor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0099] Embodiment 1, the preparation of AMP functionalized nanomaterial

[0100] Firstly, a fluorescence resonance energy transfer fluorescent nanomaterial was prepared. The nanomaterial poly2-methoxy-5-((2-ethylhexyl)oxy)-p-phenylenevinylene](MEH-PPV) was used as the matrix, combined with the near-infrared dye NIR775 and polystyrene graft Ethylene oxide functionalized with carboxyl groups (PS-PEG-COOH), prepared by nano-co-precipitation method to prepare fluorescence resonance energy transfer fluorescent nanomaterials NPs with exposed carboxyl groups on the surface (such as figure 1 b). Specifically, 5ml of tetrahydrofuran mixture (containing MEH-PPV, 40μg ml -1 ; NIR-775, 0.6 μg ml -1 ; PS-PEG-COOH, 60 μg ml -1 ) was quickly added to 10ml of ultra-pure water, and ultrasonic treatment was performed at the same time for about 15 minutes. The prepared solution is the near-infrared nanometer material generated in the self-assembly mode. Use a rotary evaporator to remove tet...

Embodiment 2

[0107] Embodiment 2, the characterization of AMP functionalized nanomaterials

[0108] The results of transmission electron microscopy showed that NPs-AMP and NPs were round and uniform in size (such as figure 2 a, image 3 a). Dynamic light scattering detects the hydrated particle size of nanoparticles, and the results show that after coupling AMP-HDA, the particle size of nanomaterials increases from 53nm to 57nm (such as figure 2 b).

[0109] Gel electrophoresis conditions and methods: 0.5% agarose gel was used for electrophoresis, the voltage was set to 150V, and the electrophoresis time was 30min. Gel electrophoresis results showed that NPs-AMP had a slower mobility than NPs in 0.5% gel electrophoresis ( image 3 b), this is because the surface charge and particle size of nanomaterials are changed after coupling AMP-HDA. These results indicated that AMP was successfully coupled to the surface of nanomaterials.

[0110] The number of AMPs that can be coupled to eac...

Embodiment 3

[0113] Example 3, NPs-AMP targets colon cancer cells at the cellular level

[0114] The present inventors first tested the targeting ability of NPs-AMP to typical colon cancer cell CW-2 by using flow cytometry, taking primary normal intestinal cells isolated from BALB / c mice as negative cells, and NPs as negative cells. For negative materials, a certain concentration of targeted (NPs-AMP) and non-targeted (NPs) nanomaterials were mixed in fresh medium to replace the original cell culture medium, and flow cytometric detection was performed after culturing for a certain period of time. The flow cytometric detection pretreatment method and the on-machine detection steps are as follows: (1) Discard the medium, and wash the adherent cells in the well plate with PBS. (2) Add trypsin to the 6-well plate to digest the cells. When most of the cells are about to fall off, it indicates that the cells have been fully digested. Then add PBS / 10% FBS in a warm bath to stop the digestion, and...

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Abstract

The invention relates to construction and application of adenosine 5'-monophosphate mediated nano transmission system for a target tumor and discloses novel compound adenosine 5'-monophosphate capable of targeting tumor cells. The compound plays the targeting role by identifying adenosine A1 receptors expressed on the surfaces of the tumor cells, can be connected with a few detectable markers such as fluorescent marks for preparation of a targeting tumor diagnosis preparation and can also be connected with a few substances with tumor inhibition activity for preparation of a targeting tumor inhibition preparation.

Description

technical field [0001] The invention relates to the field of biomedical engineering, more specifically, the invention relates to the construction and application of adenosine 5'-monophosphate-mediated tumor-targeting nano-delivery system. Background technique [0002] Targeted diagnosis and treatment of tumors has always been a research hotspot in the field of medicine. Although there are many ligand-receptor-dependent targeting ligands that can be used in tumor-targeted delivery systems, so far, there are still few successful clinical applications. In order to better improve the specificity of tumors, more and more attention has been paid to active targeting strategies using active targeting ligands. The active targeting ligands studied in the prior art mainly include monoclonal antibodies, proteins, Peptides, carbohydrates, nucleic acid aptamers and small molecules, etc. [0003] For example, nanomaterials, which are more and more used in the field of tumor imaging and t...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K47/22A61K9/14A61P35/00G01N33/58G01N33/574G01N21/64
Inventor 刘琴张元兴代同成
Owner EAST CHINA UNIV OF SCI & TECH
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