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Compound extracted from tinospora sinensis, and preparation method thereof

A compound, the technology of biscuit bile, which is applied in the field of compound and its preparation, can solve the problems such as separation and purification of monomers, and achieve the effects of accelerating comprehensive utilization and development, saving time, and good application prospects

Active Publication Date: 2017-03-08
GUANGDONG LUOFUSHAN SINOPHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the literature reports in the prior art, about the extraction process of the chemical components of Chinese green ox gall, such as: Ren Yanli, Tang Qianrui, Zhang Zhen. Research on the chemical components of Chinese green ox gall [J] Natural Product Research and Development, 2008, 20:278-279, 282, in the literature, 95% ethanol was used for reflux extraction, followed by extraction with ethyl acetate and separation by silica gel column chromatography, but palmatine and pseudopalmatine were not obtained from it; Han Dan. Qingfeng Research on the extraction and preparation process of rattan and the quality control method of rattan wide tendons [D]. Beijing: Beijing University of Traditional Chinese Medicine, 2014, using hydrochloric acid-70% ethanol reflux extraction combined with neutral alumina column chromatography for the enrichment of alkaloids, but The separation and purification of the monomer has not been achieved

Method used

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  • Compound extracted from tinospora sinensis, and preparation method thereof
  • Compound extracted from tinospora sinensis, and preparation method thereof
  • Compound extracted from tinospora sinensis, and preparation method thereof

Examples

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Effect test

Embodiment 1

[0046] According to an embodiment of the present invention, the preparation method for extracting and separating Chinese green cattle choline from Chinese green cattle bile comprises the following steps:

[0047] (1) Crushing the raw material of Chinese ox bile medicinal material: take 100 g of Chinese ox bile medicinal material, and pulverize to a particle size of 50 meshes;

[0048] (2) Preparation of Chinese ox bile extract: take the crushed Chinese ox bile, add about 1 L of 85% ethanol acidified to pH=2 with hydrochloric acid, heat under reflux for 3 times, combine the alcohol extracts, and add NaOH solution to adjust pH=7 and concentrated to a thick paste, and the solvent was recovered; after drying under reduced pressure at 60° C. to dryness, about 19.7 g of Chinese ox gall extract was obtained;

[0049] (3) Separation and purification of C. sinensis choline: adding an appropriate amount of ethyl acetate to the C. sinensis bile extract to dissolve, the solution is stirre...

Embodiment 2

[0052] According to an embodiment of the present invention, the preparation method for extracting and separating Chinese green cattle choline from Chinese green cattle bile comprises the following steps:

[0053] (1) Crushing the raw material of Chinese ox bile medicinal material: take 100 g of Chinese ox bile medicinal material, and pulverize to a particle size of 200 meshes;

[0054] (2) Preparation of Chinese ox bile extract: take the crushed Chinese ox bile, add about 1 L of 40% ethanol acidified to pH=4 with acetic acid, heat under reflux for 3 times, combine the alcohol extracts, and add NaOH solution to adjust pH=7 and concentrated to a thick paste, the solvent was recovered, and after drying under reduced pressure at 60°C to dryness, about 20.4 g of Chinese ox gall extract was obtained;

[0055] (3) Separation and purification of C. sinensis choline: adding an appropriate amount of ethyl acetate to the C. sinensis bile extract to dissolve, the solution is stirred into ...

Embodiment 3

[0058] According to an embodiment of the present invention, the preparation method for extracting and separating Chinese green cattle choline from Chinese green cattle bile comprises the following steps:

[0059] (1) Crushing the raw material of Chinese green ox bile: take 100 g of Chinese green ox bile and pulverize it to a particle size of 150 mesh;

[0060] (2) Preparation of Chinese ox bile extract: take the crushed Chinese ox bile, add about 1 L of 80% ethanol acidified to pH=3 with sulfuric acid, heat under reflux for 3 times, combine the alcohol extracts, and add NaOH solution to adjust pH=7 and concentrated to a thick paste, the solvent was recovered, and after drying under reduced pressure at 60°C to dryness, about 20.9 g of Chinese ox gall extract was obtained;

[0061](3) Separation and purification of C. sinensis choline: adding an appropriate amount of ethyl acetate to the C. sinensis bile extract to dissolve, the solution is stirred into about 24 g of silica gel,...

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Abstract

The present invention discloses a compound named tinospora sinensis alkali. The present invention further discloses a preparation method for extracting and separating the tinospora sinensis alkali from tinospora sinensis. The preparation method comprises: extracting with an acidic alcohol, carrying out concentration redissolution, directly performing column chromatography, collecting the eluent, and re-crystallizing to obtain the high-purity tinospora sinensis alkali. According to the present invention, with the preparation method, palmatine having the purity of up to 90-95% and pseudopalmatine having the purity of up to 90-95% can be separated; and the preparation method has characteristics of simple process, good stability and good reproducibility, can concurrently obtain the pure compound products such as the tinospora sinensis alkali, the palmatine and the pseudopalmatine in one time, further has advantages of time saving and consumption reducing, is suitable for industrial production, provides positive significance for the acceleration of the comprehensive utilization and development of the tinospora sinensis herb resource, and has good application prospects.

Description

technical field [0001] The invention relates to a compound and a preparation method thereof, in particular to a compound Chinese green cattle choline extracted from Chinese green cattle bile and a preparation method thereof. Background technique [0002] Tinospora sinensis (Lour.) Merr. is the dry rhizome of the Chinese green cattle gallbladder, also known as wide tendon rattan, stretch tendon rattan, green tendon rattan, etc. It is bitter in taste, slightly cold, and returns to the liver. Meridian, has the effect of relaxing tendons and activating collaterals, dispelling wind and relieving pain, mainly used for rheumatic arthralgia, muscle spasm, unfavorable flexion and extension, bruises, mainly produced in Guangdong, Guangxi, Yunnan, Hunan and other places, and is a commonly used Chinese herbal medicine in Guangdong and Guangxi. . [0003] Palmatine and pseudopalmatine belong to the quaternary ammonium alkaloids, and the molecular structures of the two are as follows. I...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D455/03
CPCC07D455/03
Inventor 丁扬洲冯少斌张清民
Owner GUANGDONG LUOFUSHAN SINOPHARM
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