Rubus-chingii seed breeding method

A technology of palm leaf raspberry and seeds, applied in the field of breeding, can solve the problems of low reproduction coefficient, difficult reproduction, irregular germination, etc., and achieve the effect of overcoming low reproduction coefficient, large reproduction coefficient and easy operation

Inactive Publication Date: 2017-03-22
JIANGXI TIANHAI SCI & TECH DEV GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, at present, most of the raw materials used in production come from the wild, and the standardized planting is in its infancy, and the seedlings are basically excavated from wild resources, transplanted and propagated by division, and the reproduction coefficient is low.
However, the seeds of Raspberry palmate are light in weight, small in size, deeply dormant, and difficult to reproduce. The rapid propagation system of Raspberry palmate seed has not been established, which is not conducive to the development of Raspberry palmate industry, and is not conducive to the study of its heterosis and genetic rules.
[0005] According to the "Journal of Yunnan Agricultural University" No. 3, 2007, Duan Xiu'an, Wang Shiyu, and Zhu Ying'an reported in the article "The Effects of Different Chemical Agents on the Germination Rate of Raspberry Seeds and the Plant Height of Seedlings". The highest germination rate is only 31%; "Economic Forest Research" 2001 No. 4 period, Fu Shunhua, Wu Jiasen, Yu Yongqing and Zhu Yuqiu etc. report in the article of " palm leaf raspberry seed characteristics and germination test research ", seed germination rate 50%, but it needs to be stored in low-temperature wet sand for 265 days, which takes a long time and requires long-term cultivation at low temperature in the refrigerator. In addition, the germination is not uniform, which increases the difficulty of seedling management including seedling transplantation, which is labor-intensive and time-consuming. This is extremely detrimental to the rapid propagation of palmate raspberry seeds

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The method for described palm leaf raspberry seed propagation comprises the steps:

[0060] (1) Pretreatment: Pick ripe raspberry fruits with palm leaves, remove sepals and fruit stalks, leave spherical aggregated achenes, crush them, rinse off the pulp with clean water, and dry them at a temperature of 23-25°C and a humidity of 20°C Save at -50%;

[0061] (2) Acid etching: Mix 98% concentrated sulfuric acid with the pretreated seeds, soak for 25 minutes, rinse with water for 10 minutes, and gently rub off the carbonized part;

[0062] (3) Potassium permanganate treatment: the seeds after the acid etching are mixed with a mass fraction of 0.2% potassium permanganate solution, soaked for 10 minutes, rinsed 3-5 times with sterile water, and dried;

[0063] (4) Gibberellin treatment: soak the seeds treated with potassium permanganate in a mixed solution of 2 mg / L gibberellin and 34 mg / L potassium nitrate for 24-36 hours;

[0064] (5) Low-temperature sand storage: bury th...

Embodiment 2

[0068] The method for described palm leaf raspberry seed propagation comprises the steps:

[0069] (1) Pretreatment: Pick ripe palmate raspberry fruits, remove sepals and fruit stalks, leave spherical aggregated achenes, crush them, rinse off the pulp with clean water, and dry them at a temperature of 20-23°C and a humidity of 35°C. Save at -60%;

[0070] (2) Acid etching: Mix 75% concentrated sulfuric acid with the pretreated seeds, soak for 15 minutes, rinse with water for 8 minutes, and gently rub off the carbonized part;

[0071] (3) Potassium permanganate treatment: mix the seeds after acid etching with a mass fraction of 0.1% potassium permanganate solution, rinse them twice with sterile water after soaking for 20 minutes, and dry them;

[0072] (4) Gibberellin treatment: soak the seeds treated with potassium permanganate in a mixed solution of 1 mg / L gibberellin and 20 mg / L potassium nitrate for 45 hours;

[0073] (5) Low-temperature sand storage: bury the seeds treat...

Embodiment 3

[0077] The method for described palm leaf raspberry seed propagation comprises the steps:

[0078] (1) Pretreatment: Pick ripe raspberry fruits with palm leaves, remove sepals and fruit stalks, leave spherical aggregated achenes, crush them, rinse off the pulp with clean water, and dry them at a temperature of 26-30°C and a humidity of 15°C. Save at -40%;

[0079] (2) Acid etching: Mix and stir 85% concentrated sulfuric acid with the pretreated seeds, soak for 35 minutes, rinse with water for 30 minutes, and gently rub off the carbonized part;

[0080] (3) Potassium permanganate treatment: mix the seeds after acid etching with a mass fraction of 0.8% potassium permanganate solution, rinse with sterile water 10 times after soaking for 6 minutes, and dry;

[0081] (4) Gibberellin treatment: soak the seeds treated with potassium permanganate in a mixed solution of 10 mg / L gibberellin and 40 mg / L potassium nitrate for 15 hours;

[0082] (5) Low-temperature sand storage: bury the...

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Abstract

The invention belongs to the breeding technology, and particularly relates to a rubus-chingii seed breeding method. The rubus-chingii seed breeding method includes the following steps that seeds are pretreated and subjected to acid etching, potassium permanganate treatment, gibberellin treatment and low-temperature stratification, then sowing, seedling raising and cultivation are carried out, and rubus chingii can be obtained. The rubus-chingii seed breeding method is simple, economical and rapid, seed dormancy of the rubus chingii is broken, the breeding coefficient is increased, and a rapid breeding system of the rubus chingii is completed.

Description

technical field [0001] The invention belongs to breeding technology, in particular to a method for seed propagation of raspberry palmate. Background technique [0002] Palm leaf raspberry (Rubus chingii Hu) is a "raspberry" included in the "Chinese Pharmacopoeia". It belongs to the genus Rubus in the family Rosaceae. , has been widely used in pharmaceuticals, health care products, cosmetics and chemical industries. [0003] Recent studies have shown that raspberry palmate is a very promising plant resource. The roots, stems and leaves of raspberry palmate can be used as medicine; the immature green fruit can be used as traditional Chinese medicine after processing. The fruit tastes sweet and delicious. Sour, warm in nature, returns to kidney, bladder meridian, has the functions of nourishing kidney, strengthening essence, reducing urination, etc. It is used for diseases such as kidney deficiency, frequent urination, impotence, premature ejaculation, and spermatorrhea. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01C1/00A01C1/08
CPCA01G17/005A01C1/00A01C1/08
Inventor 闫翠香祝天才夏昀邵小明
Owner JIANGXI TIANHAI SCI & TECH DEV GRP CO LTD
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