Fusion protein, production method thereof, and production method of transgenic rice

A technology of fusion protein and production method, applied in the field of fusion protein, can solve problems such as inability to express a large amount of fusion protein, and achieve the effect of improving expression and promoting expression

Active Publication Date: 2017-03-22
SHENZHEN RES INST THE CHINESE UNIV OF HONG KONG
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  • Abstract
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Problems solved by technology

[0013] However, the existing technology has not been able to achieve a large amount of expression of the fusio...

Method used

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  • Fusion protein, production method thereof, and production method of transgenic rice
  • Fusion protein, production method thereof, and production method of transgenic rice
  • Fusion protein, production method thereof, and production method of transgenic rice

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Embodiment Construction

[0032] In this embodiment, by designing the codon bias favorable for rice expression, the DNA sequence of the fusion protein gene is designed, and the fusion protein gene is obtained by gene synthesis. By cloning the rice seed endosperm-specific expression promoter and the rice storage protein 3'-UTR expression terminator, the Ex4-TF fusion protein gene is cloned into the rice gene expression cassette, and the transgenic rice is obtained through gene transformation, which can be obtained by Western analysis. Detect the fusion protein in transgenic rice seeds, and the fusion protein can be stably stored in the protein body of the seed endosperm. Since crop seeds are easy to store, they can be stored at room temperature for many years, and can be stored for decades in refrigerated warehouses, so the continuous production of this protein drug can be achieved without causing waste and shortages, and at the same time reducing production costs .

[0033] Such as Figure 1A , 1B Sh...

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Abstract

The invention relates to a fusion protein, a production method thereof, and a production method of transgenic rice. The fusion protein is a plant seed endosperm expressed Ex-TF fusion protein. The gene sequence of the fusion protein comprises a plant endosperm specific expression promoter (pGluB-5); a plant endosperm storage protein signal peptide (sp), an exenatide polypeptide (Exendin-4), a connection polypeptide (GGGGS)x3linker that connects Exendin-4 and human iron transport protein (hTF), a human iron transport protein (hTF), a histone label (6xHis) for the purification of proteins, and a transcription terminator (GluB-5-3'-UTR) of a plant gene (GluB-5). By utilizing the GluB-5 promoter, the fusion protein can be massively expressed in the whole endosperm. At the same time, the advantages of paddy rice such as high yield, easy plantation and management, and the like, and the advantages of paddy rice seed such as long shelf life, easy extraction, and the like, are utilized to realize the continuous production of Ex4-TF fusion protein drugs; the waste and shortage are avoided, and the production cost is reduced at the same time.

Description

technical field [0001] The present invention relates to a fusion protein, its production method, especially a plant seed expression system of recombinant exenatide fusion protein, and a production method of transgenic rice. Background technique [0002] Diabetes is a worldwide chronic multiple disease. Type 1 diabetes is caused by insufficient secretion of insulin, which regulates blood sugar, while type 2 diabetes is caused by poor utilization of insulin or insulin resistance. Insulin is a hormone secreted by pancreatic β cells to regulate the concentration of sugar in the blood. The lack or ineffective use of insulin often increases the blood sugar level of patients. If it is not treated effectively, it will cause serious complications (such as heart disease, stroke, etc.) , diabetic retinopathy, renal failure, etc.) and even death. [0003] Diabetes directly or indirectly causes millions of deaths every year. According to the World Health Organization, there are 347 mi...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/82A01H5/00
Inventor 于为常陈磊黄彪李相鲁
Owner SHENZHEN RES INST THE CHINESE UNIV OF HONG KONG
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