Fusion protein, production method thereof, and production method of transgenic rice

Abstract

The invention relates to a fusion protein, a production method thereof, and a production method of transgenic rice. The fusion protein is a plant seed endosperm expressed Ex-TF fusion protein. The gene sequence of the fusion protein comprises a plant endosperm specific expression promoter (pGluB-5); a plant endosperm storage protein signal peptide (sp), an exenatide polypeptide (Exendin-4), a connection polypeptide (GGGGS)x3linker that connects Exendin-4 and human iron transport protein (hTF), a human iron transport protein (hTF), a histone label (6xHis) for the purification of proteins, and a transcription terminator (GluB-5-3'-UTR) of a plant gene (GluB-5). By utilizing the GluB-5 promoter, the fusion protein can be massively expressed in the whole endosperm. At the same time, the advantages of paddy rice such as high yield, easy plantation and management, and the like, and the advantages of paddy rice seed such as long shelf life, easy extraction, and the like, are utilized to realize the continuous production of Ex4-TF fusion protein drugs; the waste and shortage are avoided, and the production cost is reduced at the same time.

Description

technical field [0001] The present invention relates to a fusion protein, its production method, especially a plant seed expression system of recombinant exenatide fusion protein, and a production method of transgenic rice. Background technique [0002] Diabetes is a worldwide chronic multiple disease. Type 1 diabetes is caused by insufficient secretion of insulin, which regulates blood sugar, while type 2 diabetes is caused by poor utilization of insulin or insulin resistance. Insulin is a hormone secreted by pancreatic β cells to regulate the concentration of sugar in the blood. The lack or ineffective use of insulin often increases the blood sugar level of patients. If it is not treated effectively, it will cause serious complications (such as heart disease, stroke, etc.) , diabetic retinopathy, renal failure, etc.) and even death. [0003] Diabetes directly or indirectly causes millions of deaths every year. According to the World Health Organization, there are 347 mi...

AI Technical Summary

Problems solved by technology

[0013] However, the existing technology has not been able to achieve a large amount of expression of the fusion protein in the whole endosperm, and the previous expression was only expressed in the aleurone layer or some cells

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