A multi-purpose instrument for microbial acid and gas production experiments and sulfur-containing amino acid detection experiments

Abstract

The invention relates to the field of microbe experimental facilities, and in particular discloses a multi-function instrument used for the microbe acid and gas producing experiment and the sulfur-containing amino acid detection experiment. The multi-function instrument comprises a constant temperature incubator, a fermentation test tube, an acid and gas producing experiment indicating device and a sulfur-containing amino acid detection device, wherein the acid and gas producing experiment indicating device comprises a liquid storage chamber and a seal end cover, the middle part of the seal end cover sinks, the sinking part is in sealed connection with a gas collecting tube, a water injecting hole and an air hole are formed in the seal end cover, and a puncture device and a seal rubber sleeve are in sealed connection to the bottom of the liquid storage chamber; the sulfur-containing amino acid detection device comprises a detection chamber, a rubber plug is plugged to the lower opening of the detection chamber, a puncture device is inserted into the rubber plug, and a rubber seal sleeve is arranged at the lower end of the detection chamber; a filter paper strip coiled to form the barrel shape is plugged in the detection chamber. The multi-function instrument has the advantages that the size is small, the taking for use is convenient, the operation is simple, the efficiency is high, the space is saved, the experiment is simple and rapid, the result is reliable, and multiple purposes are realized.

Description

(1) Technical field [0001] The invention relates to the field of microbial experimental equipment, in particular to a multi-purpose instrument for microbial acid and gas production experiments and sulfur-containing amino acid detection experiments. (2) Background technology [0002] At present, when conducting acid and gas production experiments of microbial fermentation such as glucose and lactose, because of observing the gas production, it is often necessary to invert a small-caliber fermentation small tube in a large test tube containing liquid medium, and the operation is very cumbersome. Use a pipette to fill the small fermentation tube with the liquid medium first, and then quickly pour it into the large test tube already filled with the liquid medium. Even if you are careful, air bubbles will be generated if you do not handle it carefully. At this time, you must take out the small fermentation tube and start again Placement is time-consuming and laborious, and the ef...

AI Technical Summary

Problems solved by technology

[0002] At present, when conducting acid and gas production experiments of microbial fermentation such as glucose and lactose, because of observing the gas production, it is often necessary to invert a small-caliber fermentation small tube in a large test tube containing liquid medium, and the operation is very cumbersome. Use a pipette to fill the small fermentation tube with the liquid medium first, and then quickly pour it into the large test tube already filled with the liquid medium. Even if you are careful, air bubbles will be generated if you do not handle it carefully. At this time, you must take out the small fermentation tube and start again Placement is time-consuming and laborious, and the effect is not good; at the same time, because the fermentation liquid medium contains different sugars (such as glucose or lactose), after an experiment, it is difficult to rinse with a test tube brush due to the small diameter of the fermentation tube. It is too time-consuming and energy-consuming to rinse thoroughly with lotion every time. If it is not rinsed well, once a small amount of glucose is contained in the lactose fermentation tubule, it will seriously affect the experimental results and cause gas production in experiments that should not have produced gas. False Positive Results for Gas

[0003] In the detection experiment of sulfur-containing amino acids, a small amount of FeSO is generally added to the preparation of solid or semi-solid medium 4 or lead acetate (because H 2 S and FeSO 4 or lead acetate can generate black FeS or lead sulfide precipitate), through puncture inoculation, see if there is black around the puncture line, if there is, it means that the amino acid is a sulfur-containing amino acid; if there is no black around the puncture line, it means the amino acid is a sulfur-free amino acid; but due to FeSO 4 or lead acetate is added to the medium, even if the produced H 2 There is a lot of S, and it is difficult to see the appearance of black, and it is also difficult to observe a small amount of black, which makes it easy to appear false negatives, which often brings difficulties to the detection of sulfur-containing amino acids.

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