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Vancomycin bacterial strain and method for fermentation production of vancomycin

A vancomycin and strain technology, applied in fermentation, bacteria and other directions, can solve the problem of time-consuming and labor-intensive

Inactive Publication Date: 2017-03-22
江苏海阔生物医药有限公司
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Problems solved by technology

Researchers have obtained some vancomycin-producing strains by isolating, breeding or mutating the strains by various means, but it is time-consuming and labor-intensive

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  • Vancomycin bacterial strain and method for fermentation production of vancomycin

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Embodiment

[0032] The present invention comprises the following steps:

[0033] a. The vancomycin production strain is carried out in a liquid fermentation medium culture, and the liquid fermentation seed medium is composed of yeast powder 4g / L, malt extract 5g / L, peptone 12g / L, glucose 18g / L, and pH is 6.8; The culture conditions are: 30°C, liquid volume 189mL, shaker speed 187rpm, culture for 48h; liquid fermentation medium consists of dextrin 150g / L, potato protein 25g / L, soybean powder 25g / L, dipotassium hydrogen phosphate 0.4g / L, defoamer 2g / L, sodium chloride 1.3g / L, pH 7.2, culture condition is 28°C, liquid volume 245mL, shaker speed 220rpm, culture 110h, the obtained solution is the eluent containing net antibiotic activity ;

[0034] b. Precipitate the eluent containing net antibiotic activity obtained in step a in a precipitation container to obtain a crystal slurry, and stir the crystal slurry to continuously adjust the pH value to the required level until complete precipita...

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Abstract

The invention discloses a vancomycin bacterial strain and a method for fermentation production of vancomycin. The vancomycin producing bacterial strain is cultured in a liquid fermentation culture medium, and an obtained solution is a pure antibiotic-containing active eluate. A ribosome mutagenesis breeding means is adopted for breading the vancomycin producing bacterial strain, and the amycolatopsis orientalis vancomycin producing bacterial strain is obtained; the vancomycin production capacity of the bacterial strain is increased by 7.12 times than a starting bacterial strain and is up to 8.9 g / L. Through the passage stability study, the vancomycin yield stability is as high as 92.7% after continuous passage is performed for 5 times.

Description

technical field [0001] The invention relates to the technical field of producing vancomycin, in particular to a vancomycin strain and a method for producing vancomycin by fermentation. Background technique [0002] Vancomycin was obtained from Indonesian soil in 1956 by McCormick et al. (McCormick MH, Stark WM, Plttenger GE, et al. A glycopeptide antibiotic with resistance to Gram-positive bacteria isolated from the fermentation broth of a selected strain called Amycolatopsis orientalis. Twenty years after its introduction, vancomycin was reserved only for the treatment of severe severe disease caused by a small number of drug-resistant Staphylococcus aureus due to the marketed use of penicillins and cephalosporins and their strong oto-nephrotoxicity. Infectious diseases, clinical use is rare. Later, with the extensive use of β-lactam antibiotics, infections caused by methicillin-resistant Staphylococcus aureus (MRSA) became prevalent. From 1998 to 2000, MRSA accounted fo...

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Application Information

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IPC IPC(8): C12N1/20C12P21/02
Inventor 苗玉武吕祖平刘永刚王丹丹刘言华
Owner 江苏海阔生物医药有限公司
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