Construction method of SD (Sprague Dawley) rat pancreas duct epithelial cell model

Abstract

The invention discloses a construction method of an SD (Sprague Dawley) rat pancreas duct epithelial cell model. The construction method adopts a rat and is characterized by further comprising steps as follows: animal selection and anesthesia, surgical separation of a main pancreatic duct of the rat, chemical digestion of the main pancreatic duct and cell type identification. According to the construction method, the main pancreatic duct is directly separated under the naked eyes according to a pancreatic anatomical structure of the SD rat, the separation recovery rate of epithelial cells of the pancreatic duct is higher, the obtained epithelial cells of the pancreatic duct are less irritated, and stable passage can be realized. The method is simple and convenient, the success rate is high, and the novel cell model with rich and reliable sources is provided for research of diseases of pancreas.

Description

technical field [0001] The invention relates to a cell model, especially a method for constructing a SD rat pancreatic ductal epithelial cell model. Background technique [0002] Pancreatic lesions are one of the diseases with the most complicated pathogenesis in human diseases. The inflammatory lesions are critical and difficult to treat; while the neoplastic diseases are difficult to detect early, with high mortality and poor treatment effect. Most pancreatic inflammation and tumors are caused by ductal epithelial cell lesions. However, it is very difficult to isolate and culture pancreatic ductal epithelial cells, which greatly limits the research on pancreatic lesions. At present, foreign studies on the in vitro culture of primary rat pancreatic ductal epithelial cells are mainly concentrated in the 1970s. The main method is: directly excise the head tissue of the rat pancreas, separate the main pancreatic duct area under a stereomicroscope, and place it in the After di...

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that it is difficult to select the sieve, and the Ficoll solution needs to be freshly prepared, the equipment requirements for gradient centrifugation are high, the operation is cumbersome and the success rate is not high

The other is to directly isolate the pancreatic tissue of SD suckling mice, cut it into pieces, digest it with type IV collagenase, and collect the centrifuged sediment for primary culture. The biggest disadvantage of this method is that most of the obtained pancreatic islet cells and ductal epithelial cells low rate of acquisition

However, the existing methods for isolating pancreatic ductal epithelial cells are too cumbersome to operate and have a low success rate.

Current methods for isolating ductal epithelial cells are not adequate for further investigation of pancreatic duct-associated lesions