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A siderophore high-yielding bacterium and its application in remediation of heavy metals in farmland polluted soil

A technology for polluted soil and siderophore, which is applied in the field of environmental pollution bioremediation, can solve the problems of lack of strains, limited high-yielding strains of siderophores, and few strains.

Active Publication Date: 2019-07-19
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, among the reported strains, siderophore high-yield strains are limited; strains with multiple advantages and characteristics (such as multiple heavy metal resistance and resistance to pathogenic fungi) are lacking; strains that can actually strengthen the ability of plants to absorb heavy metals in soil very few

Method used

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  • A siderophore high-yielding bacterium and its application in remediation of heavy metals in farmland polluted soil
  • A siderophore high-yielding bacterium and its application in remediation of heavy metals in farmland polluted soil
  • A siderophore high-yielding bacterium and its application in remediation of heavy metals in farmland polluted soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Isolation of Pseudomonas sp.S17 and Test of Siderophore Production Ability

[0029] Collect 100g of soil from the experimental field in Zhejiang University of Science and Technology, Hangzhou City, Zhejiang Province by the general five-point sampling method, put it in a sterile envelope, and bring it back to the laboratory quickly.

[0030] General CAS detection solution and MSA liquid medium are based on the report of Chen Shaoxing et al. ) preparation. The solid detection plate is solidified by adding 5% CAS detection solution and 2% agar powder into MSA liquid medium.

[0031] Dilute the soil sample by 10-fold decreasing method, and spread it on one-fifth LB solid plate (that is, all nutrients are 1 / 5 of the general LB medium). Cultivate at 25°C. After colonies appear, pick out the colonies of different forms one by one with a sterile toothpick and transfer them to a new one-fifth LB solid plate until they are purified into single colonies. All the pure s...

Embodiment 2

[0038] Example 2: Antibiotic susceptibility test and pathogenic fungus antagonistic ability test of Pseudomonas sp.S17

[0039] The antibiotic susceptibility test adopts the filter paper method, and five commonly used antibiotics such as ampicillin, tetracycline, chloramphenicol, kanamycin and streptomycin are selected. The size is used as a criterion for determining sensitivity or resistance (anti-)drugs. The test results given in Table 3 show that the strain S17 is sensitive to the above-mentioned tested antibiotics in varying degrees.

[0040] Table 3. Antibiotic susceptibility test results of bacterial strain S17

[0041]

[0042] Note: If the diameter of the inhibition zone is larger than the upper limit of the moderately sensitive range, it is highly sensitive, and if it is smaller than the lower limit of the sensitive range, it is resistant.

[0043] This example shows that when the Pseudomonas sp.S17 bacterium is released into the natural environment, it will not ...

Embodiment 3

[0050] Embodiment 3: The heavy metal minimum inhibitory concentration (MIC) test of Pseudomonas sp.S17

[0051] The design of the MIC experiment was carried out according to the research of Filali et al. (Filali BK, Taoufik J, Zeroual Y, Dzairi FZ, Talbi M, Blaghen M. Waste water bacterial isolates resistant to heavymetals and antibiotics. Current Microbiology, 2000, 41: 151-156) , pick a single colony of S17 of equal size and after activation treatment in a culture test tube, and cultivate it at 30°C with shaking at 160r / min for 32 hours. The MIC value was determined according to the growth of the bacteria, and three replicates were set up in each group to test the tolerance of the strain to 12 metal ions. The results are shown in Table 5, the strain has multiple heavy metal tolerance (resistance), and has excellent properties.

[0052] Table 5. MIC test of strain S17

[0053]

[0054] Note: The data in the table is the average value of 3 measured values.

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Abstract

The invention belongs to the field of bioremediation of environmental pollution, in particular to a siderophore high-yielding bacterium and its application in the aspect of remediation of heavy metals in farmland polluted soil. A strain of siderophore high-producing bacteria, the bacteria is Pseudomonas ( Pseudomonas sp.), the strain S17 was preserved in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on October 12, 2016, with the number CGMCC No.13104. of the present invention Pseudomonas The sp.S17 strain has reached the highest level of siderophore-producing bacteria, and its siderophore-producing ability is the strongest among the reported strains in China. Pseudomonas sp.S17 is sensitive to commonly used antibiotics, shows different degrees of tolerance or resistance to 12 heavy metals, and has resistance to pathogenic fungi, phosphorus-dissolving ability and 3‑indoleacetic acid production ability.

Description

technical field [0001] The invention belongs to the field of bioremediation of environmental pollution, in particular to a siderophore high-yielding bacterium and its application in the aspect of remediation of heavy metals in farmland polluted soil. Background technique [0002] Heavy metal pollution in soil has become a global environmental problem, which has attracted the attention of the society and the public and needs to be solved urgently. The soil in many areas of my country (especially in East China) is polluted by heavy metals to varying degrees. The content of heavy metals such as cadmium, arsenic, copper, lead, and zinc in grains, vegetables, and fruits in many areas exceeds the standard or is close to the critical value, seriously endangering the health of the people. [0003] Soil heavy metal pollution control is imminent but difficult, mainly because heavy metals are not biodegradable like organic pollutants, and heavy metals have poor mobility in soil. Tradi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/38
CPCB09C1/10B09C2101/00C12N1/205C12R2001/38
Inventor 虞方伯管泽华单胜道钱晓云管莉菠鲍雅莉
Owner ZHEJIANG FORESTRY UNIVERSITY