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A Strain of Carboxybacter beijerinckii and Its Application

A technology of Clostridium beijerinckii and microbial strains, applied in the field of microorganisms, can solve the problems of increased energy consumption, abnormal growth of bacteria cells, low butanol yield, etc., so as to reduce the pressure of butanol recovery and avoid bacterial failure. Growth phenomena, reducing the effect of deoxidizing steps

Active Publication Date: 2020-03-17
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional acetone-butanol fermentation generally has the following problems: (1) The traditional acetone-butanol fermentation strain needs to be cultivated and fermented under strict anaerobic conditions. A little careless operation can easily enter the air and cause the bacteria to not grow normally. During the fermentation process, it is usually necessary to pass inert gas such as N 2 (2) The yield of butanol is low, only about 20% (mass fraction), which makes the cost of raw materials in the butanol fermentation process relatively high, which restricts the development of the butanol fermentation industry; (3) ) In addition to butanol, the fermentation product also has 40% by-products such as acetone and ethanol, which increases the difficulty of product separation and increases energy consumption
[0007] Chinese patent 201210089406.2 discloses a butanol-producing Clostridium beijerinckii ( Clostridium beijerinckii ) Y-3, the strain is the starting strain Clostridium beijerinckii NCIMB8052 is a mutant strain obtained by mutagenesis with ethyl methanesulfonate (EMS), which can produce bio-butanol from xylose residue as a raw material. When using xylose residue enzymatic hydrolysis solution as a carbon source, the total solvent output is 16g / L, The butanol output is 8.2g / L, which solves the problems of traditional biological fermentation to produce butanol strains and insufficient raw materials

Method used

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  • A Strain of Carboxybacter beijerinckii and Its Application
  • A Strain of Carboxybacter beijerinckii and Its Application
  • A Strain of Carboxybacter beijerinckii and Its Application

Examples

Experimental program
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Effect test

Embodiment 1

[0031] The screening process of embodiment 1 Clostridium beijerinckii XH0906 bacterial strain

[0032]In June 2012, forest soil and livestock manure were collected from the surrounding areas of Fushun City, Liaoning Province, mixed with corn stalk powder and placed in a sterile triangular flask, and an appropriate amount of sterile water was added to enrich the gas-producing bacteria anaerobically. After 2 weeks of enrichment, bubbles were found in the anaerobic culture, and 0.2 mL of the culture was inoculated into 4.8 mL of liquid medium placed in a test tube. The composition of the liquid medium is: peptone 10 g / L, beef extract 6 g / L, glucose 40 g / L, sodium chloride 0.5 g / L, ammonium sulfate 0.9 g / L, iron sulfate 0.1 g / L, magnesium sulfate 0.3 g / L, calcium chloride 0.1 g / L, pH 7.0, sterilized at 121°C for 15 minutes. After static culture for 72 h, a large number of air bubbles can be observed during the culture process. The culture fluid was centrifuged to take the super...

Embodiment 2

[0036] Example 2 Production of butanol by non-anaerobic fermentation of Clostridium beijerinckii XH0906

[0037] Liquid medium components: peptone 10 g / L, beef extract 6 g / L, glucose 40 g / L, sodium chloride 0.5 g / L, ammonium sulfate 0.9 g / L, iron sulfate 0.1 g / L, magnesium sulfate 0.3 g / L L. Calcium chloride 0.1 g / L, pH 7.0, sterilized at 121°C for 15 minutes. Solid media add 1% agar to liquid media.

[0038] Production of butanol by non-anaerobic fermentation of Clostridium beijerinckii XH0906: (1) Inoculate the Clostridium beijerinckii XH0906 on a solid medium slant, place in an anaerobic environment, and cultivate at 30°C for 24 hours; (1) Scrape 1-2 rings of the cultivated Clostridium beijerinckii from the slant and put them into the shake flask seed medium, no need to feed N 2 , 35 ℃ static culture for 24h; (3) The seed liquid cultivated in step (2) was inserted into the liquid fermentation medium with an inoculum of 10%, and no N was needed. 2 , natural pH, fermented ...

Embodiment 3

[0040] The fermentation process and operating conditions are the same as in Example 2, except that different carbon sources and nitrogen sources are used to replace the glucose, peptone or ammonium sulfate in the original culture medium. The fermentation results are shown in Table 3.

[0041] Table 3 Fermentation results with different carbon and nitrogen sources in the medium

[0042]

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Abstract

The invention discloses a butanol-producing Clostridium beijerinckii XH0906 and its application, which is classified as Clostridium beijerinckii ( Clostridium beijerinckii ), which was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on May 4, 2014, with the preservation number CGMCC No.9124. The strain can grow and ferment butanol in a non-strict anaerobic environment. It does not need to add sodium hydrochloride to the fermentation medium to remove oxygen, and it does not need to feed N during the fermentation process. 2 Maintaining an anaerobic environment avoids the phenomenon of bacteria not growing due to unclean deoxygenation and reduces energy consumption; and it has the characteristics of high butanol production and less by-products of acetone and ethanol, which reduces the pressure of subsequent butanol recovery. Reduced separation energy consumption.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a butanol-producing Clostridium beijerinckii and its application in fermentative production of butanol. Background technique [0002] Butanol is an important organic chemical raw material, which is widely used in chemical, pharmaceutical and petroleum industries. And because there are two more methylene groups than ethanol, butanol has higher hydrophobicity and lower volatility, can be mixed with gasoline in any proportion, and has a calorific value equivalent to gasoline. As a potential renewable bioenergy that can replace gasoline, butanol has attracted more and more attention from all over the world. [0003] The production process of butanol mainly includes chemical synthesis method and microbial fermentation method. With the depletion of petroleum resources, it is difficult to produce butanol by propylene oxo synthesis using petroleum as raw material, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/16C12R1/145
CPCC12N1/20Y02E50/10C12R2001/145C12N1/205C12P7/16C12P7/10
Inventor 张全曹长海高慧鹏关浩王领民乔凯
Owner CHINA PETROLEUM & CHEM CORP
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