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Method for fermenting production of butanol

A technology of butanol and fermentation bacteria, applied in the field of microorganisms, can solve the problems of restricting the development of butanol fermentation industry, unable to grow normally, increasing the difficulty of product separation, etc., so as to avoid the phenomenon of non-growth of bacteria and improve the utilization rate of carbon source. , high yield and high yield

Active Publication Date: 2018-10-23
CHINA PETROLEUM & CHEM CORP +1
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AI Technical Summary

Problems solved by technology

The traditional acetone-butanol fermentation generally has the following problems: (1) The traditional acetone-butanol fermentation strain needs to be cultivated and fermented under strict anaerobic conditions. A little careless operation can easily enter the air and cause the bacteria to not grow normally. During the fermentation process, it is usually necessary to pass inert gas such as N 2 To ensure an anaerobic environment, the energy consumption is high; (2) the butanol yield is low, only about 20% by weight, which makes the raw material cost of the butanol fermentation process high and restricts the development of the butanol fermentation industry; (3) the fermentation product In addition to butanol, there are by-products such as 40% by weight of acetone and ethanol, which increases the difficulty of product separation and improves energy consumption.
Although this strain solves the problems of traditional biological fermentation to produce butanol strains and insufficient raw materials, the fermentation process also needs to be carried out in a strict anaerobic environment, and because there are more acetone and ethanol by-products in the fermentation product, There are still defects such as high pressure for subsequent butanol recovery and high energy consumption for separation

Method used

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preparation example Construction

[0027] The preparation method of the soybean meal hydrolyzate comprises: weighing an appropriate amount of soybean meal, adding 5 times the mass of water, and mixing uniformly. Add concentrated sulfuric acid with a mass concentration of 98% according to 2% of the water volume, mix quickly and evenly, and do not partially carbonize the material. Steam was introduced to raise the temperature of the material to 100°C, and the temperature was kept for 20 hours, during which, it was stirred for 5 minutes every hour. After the hydrolysis is finished, a soybean meal hydrolyzate that is red in sauce and has a fruity aroma is obtained.

[0028] Analyze the products and by-products in the fermentation broth by liquid chromatography to calculate the concentration of the main components: liquid chromatography (Agilent 1200), the chromatographic column is Bio-Rad HPX-87H (300mm×7.8mm), mobile phase 0.005mol / L H2SO4, the flow rate is 0.6 ml / min, the temperature of the column oven is 65°C, ...

Embodiment 1

[0033] Liquid medium (including seed medium and fermentation medium) components: peptone 10 g / L, beef extract 6 g / L, glucose 45 g / L, sodium chloride 0.5 g / L, ammonium sulfate 0.9 g / L, iron sulfate 0.1 g / L, magnesium sulfate 0.3 g / L, calcium chloride 0.1 g / L, potassium dihydrogen phosphate 1 g / L, disodium hydrogen phosphate 2 g / L, p-aminobenzoic acid 0.04 g / L, vitamin B1 0.04 g / L and biotin 0.004g / L, pH 7.0, sterilized at 121°C for 15min. Add 1wt% agar to the liquid medium for solid medium.

[0034] Add the calcium carbonate of 0.5g / L in above-mentioned medium as fermentation medium.

[0035] The method of using Clostridium beijerinckii CGMCC No. 9124 to ferment and produce butanol under facultative conditions includes: (1) inoculating the Clostridium beijerinckii on a solid medium slant, placing it in an anaerobic environment, and culturing at 30°C 24h; (2) Scrape 2 rings of the Clostridium beijerinckii cultivated in step (1) from the slope and insert them into the seed medi...

Embodiment 2

[0037] According to the method of embodiment 1, difference is that calcium carbonate add-on is respectively 1g / L, 2g / L, 3g / L and 4g / L. The results are shown in Table 1.

[0038] Table 1

[0039]

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Abstract

The invention relates to a method for fermenting production of butanol. The method comprises: (1) preparing a fermentation bacteria seed liquid, wherein the fermentation bacteria is Clostridium beijerinckii XH0906; and (2) preparing a fermentation culture medium, adding calcium carbonate into the fermentation culture medium, inoculating with the seed liquid prepared in the step (1), and carrying out fermenting culture to produce butanol. According to the present invention, the butanol is subjected to fermenting production under the facultative condition, and the method has characteristics of high butanol yield, less organic acid by-product and the like.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular, relates to a Clostridium beijerinckii ) method for producing butanol by fermentation. Background technique [0002] Butanol is an important organic chemical raw material, which is widely used in chemical, pharmaceutical and petroleum industries. Butanol has two more methylene groups than ethanol. Compared with ethanol, butanol has higher hydrophobicity and lower volatility. It can be mixed with gasoline in any proportion, and has a calorific value equivalent to gasoline. As a potential renewable bioenergy that can replace gasoline, butanol has attracted more and more attention from all over the world. [0003] The production process of butanol mainly includes chemical synthesis method and microbial fermentation method. With the depletion of petroleum resources, it is difficult to produce butanol by propylene oxo synthesis using petroleum as raw material, and due to back...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/16C12R1/145
CPCC12N1/20C12P7/16Y02E50/10
Inventor 张全曹长海关浩高慧鹏王领民乔凯
Owner CHINA PETROLEUM & CHEM CORP
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