Method for separating and purifying 7-hydroxyl tropolone from bacteria supernatant

Abstract

The invention discloses a method for separating and purifying 7-hydroxyl tropolone from bacteria supernatant. The method comprises the following steps: (1) collecting fermentation liquor of pseudomonas donghuensis HT (the accession number is CCTCC NO: M 2015481), centrifuging and then filtering supernatant to obtain supernatant of a bacteria solution; (2) extracting the supernatant of the bacteria solution with ethyl acetate, retaining a water phase of the lower layer, regulating the pH of the water phase to be acidic, extracting the water phase with the ethyl acetate again, and collecting an organic phase of the upper layer; (3) evaporating the ethyl acetate in the organic phase to dryness under reduced pressure, and dissolving the rest of substances in anhydrous ethanol; and (4) carrying out chromatographic purification by using Sephadex LH-20, wherein a mobile phase is the anhydrous ethanol; collecting components with ferrous chelate activity, and evaporating the anhydrous ethanol to dryness under reduced pressure to obtain the 7-hydroxyl tropolone. By the method, the 7-hydroxyl tropolone of which the purity reaches 90% or above can be obtained, and the recovery rate is greater than 60%.

Description

technical field

[0001] The invention belongs to the technical fields of bioengineering and biochemistry, and in particular relates to a method for separating and purifying 7-hydroxytropenolone from bacterial supernatant. Background technique

[0002] 7-Hydroxytropenolone compounds are a class of natural products belonging to the tropotrienone-type compounds, which exhibit multiple biological activities in many aspects. Due to the addition of a hydroxyl group at the ortho position of the ketone group, 7-hydroxytropolone exhibits stronger inhibitory activity relative to tropolone. Some studies have shown that 7-hydroxytropenolone and its derivatives β-thujaplicinol (β-thujaplicinol), 3,7-dihydroxytropenolone, puberulic acid , Puberulonic acid can be used as a potential therapeutic drug for many diseases. 7-Hydroxytropenolone showed strong inhibitory activity against Gram-positive bacteria, such as Staphylococcus aureus ( Staphylococcus aureus ) and Micrococcus luteus ( Mi...

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