Method for inducing coralline algae to increase exopolysaccharide secretion intensity

Abstract

The invention relates to a method for inducing coralline algae to improve exopolysaccharide secretion intensity. The method comprises the steps that a zinc methionine organic microelement chelate is added to sea water used for culturing the coralline algae; the mixture is fully stirred to be uniform, so that the final concentration of the zinc methionine organic microelement chelate in a culture solution reaches 0.05-0.15 mg.L<1>; and the coralline algae is cultured. A preparation method of the zinc methionine organic microelement chelate comprises the steps that methionine is dissolved into distilled water, basic zinc carbonate is added, and stirring is conducted so that the materials can react; a hydrochloric acid solution is added, and heating is conducted till all the methionine is dissolved, wherein a solution is transparent and in light yellow; pH is adjusted, and filtration is conducted; and sedimentation, centrifugation and drying are conducted, so that the zinc methionine organic microelement chelate is obtained. According to the method, organic microelements are added to the sea water used for culturing the coralline algae, physiological activity of the coralline algae can be obviously promoted, exopolysaccharide secretion intensity of the coralline algae can be improved, and thus utilization value of coralline algae resources is increased; and environmental adaptability and adverse situation resistance of the coralline algae are improved.

Description

technical field [0001] The invention belongs to the fields of marine biotechnology and agricultural resource development, and in particular relates to a method for inducing coralline algae to increase the secretion intensity of exopolysaccharides. Background technique [0002] Scientific research has confirmed that there are a total of 18 elements that make up the body, which are divided into macro elements and trace elements. Macro elements refer to elements whose content accounts for more than 0.01% of body weight; trace elements refer to elements whose content accounts for less than 0.01% of body weight. Among them, the six elements of carbon, nitrogen, oxygen, hydrogen, calcium and phosphorus account for 99% of the total amount of the body, which are called constant elements, and the remaining 12 kinds of elements are about 1%, so they are called trace elements. They are iodine, Sulfur, Sodium, Chlorine, Iron, Copper, Potassium, Magnesium, Manganese, Cobalt, Zinc, Selen...

AI Technical Summary

Problems solved by technology

[0009] However, at present, in the development and utilization of seaweed exopolysaccharide resources, biotechnology that induces the secretion of algae polysaccharides with trace elements is rarely used, especially through the preparation and application of organic trace elements to promote the secretion of exopolysaccharides in coralline algae and improve the production of exopolysaccharides. Biological activity, thereby significantly enhancing the ability of coralline algae to produce high-activity marine biological product exopolysaccharides, and related technologies to improve the utilization value of coralline algae marine biological resources are still blank

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