Hemoglobin coloring agent and preparation method thereof

A technology of hemoglobin and colorant, applied in the field of hemoglobin colorant and its preparation, can solve problems such as poor stability, hemoglobin is sensitive to light, limit the development of processing and application, achieve good stability, improve stability, and increase practicability and application-wide effects

Inactive Publication Date: 2017-04-19
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because hemoglobin is sensitive to light and has poor stability, the development of its processing and application is limited.

Method used

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  • Hemoglobin coloring agent and preparation method thereof
  • Hemoglobin coloring agent and preparation method thereof
  • Hemoglobin coloring agent and preparation method thereof

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preparation example Construction

[0027] The invention provides a method for preparing a hemoglobin colorant, comprising: step 1, extracting hemoglobin aqueous solution from fresh blood of livestock and poultry; step 2, adding arginine or lysine to the hemoglobin aqueous solution to obtain a mixed solution, Wherein, the ratio of arginine or lysine in the mixed solution is 4-12 mg / mL, and the pH value is adjusted to 9.5-12; step 3, drying the mixed solution. As an antioxidant, arginine or lysine can protect hemoglobin, prevent hemoglobin from being oxidized to methemoglobin during the drying process, maintain the divalent state of iron, and enable it to maintain a bright red color. The invention determines the optimal process condition for preparing hemoglobin with high stability, and provides a basis for large-scale production and processing.

[0028] In a preferred embodiment, in the preparation method of the hemoglobin coloring agent, in the second step, when arginine or lysine is added to the hemoglobin aqu...

Embodiment 1

[0043] The hemoglobin colorant of embodiment one low concentration arginine (arginine concentration is 4mg / mL)

[0044] (1) Blood collection: After slaughtering the sheep that passed the quarantine, the blood was collected in a container previously equipped with 5% anticoagulant, and the volume ratio of blood and anticoagulant was 1:10 and quantitatively mixed;

[0045] (2) Centrifugation: centrifuge the anticoagulated blood at 6000g for 30min at 4°C, and collect the red blood cells in the lower layer;

[0046] (3) Crude extraction of hemoglobin: RBCs were washed 3 to 5 times with normal saline, ultrasonic power was 250w, frequency was 25kHz, ultrasonic treatment was for 30min, centrifugal force was 8000g, and temperature was 4°C-6°C.

[0047] (4) Refined hemoglobin extraction: Hemoglobin crude extract was heated in a 60°C water bath for 30 minutes, cooled and then centrifuged with a centrifugal force of 8000g and a temperature of 4°C to 6°C.

[0048] (5) Treatment of hemoglo...

Embodiment 2

[0050] The hemoglobin colorant of embodiment two high-concentration arginine (arginine concentration is 12mg / mL)

[0051] Wherein the steps are the same as in Embodiment 1. In step (5), the concentration of added arginine is 12 mg / mL.

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Abstract

The invention discloses a preparation method of a hemoglobin coloring agent. The preparation method of the hemoglobin coloring agent comprises the steps that firstly, a hemoglobin water solution is extracted from fresh blood of livestock and poultry; secondly, arginine or lysine is added to the hemoglobin water solution, so that the mixed solution is obtained, wherein the ratio of the arginine or the lysine in the mixed solution is 4-12 mg/mL, and the pH value is adjusted to be 9.5-12; and thirdly, the mixed solution is dried. In the preparation process, the arginine or lysine is added, the arginine or lysine is used as an antioxidant and can protect hemoglobin, and therefore the hemoglobin can be kept in bright red. According to the preparation method of the hemoglobin coloring agent, the best technological conditions for preparation of hemoglobin high in stability are determined, and the basis is provided for mass production and processing. The prepared novel hemoglobin coloring agent product has good stability in different concentrates of NaCl and solutions of different gradients of pH.

Description

technical field [0001] The invention relates to a hemoglobin colorant and a preparation method thereof. Background technique [0002] In 2015, the total number of pigs, cattle and sheep slaughtered in my country reached 1 billion, the total number of poultry slaughtered exceeded 13 billion, and the total amount of livestock and poultry blood reached 3 million tons. It is the country with the richest livestock and poultry blood resources in the world. However, for many years, the utilization rate of livestock and poultry blood in my country has been low. It is only partially used for processing traditional blood products such as blood sausage and blood tofu, and as animal feed in the form of blood meal. The remaining about 70% of livestock and poultry blood is discharged in the form of sewage, which not only causes Waste of resources, and cause serious environmental pollution. Hemoglobin is a kind of natural red pigment. The production raw material comes from livestock and po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00
CPCC09B61/00C09B67/0092
Inventor 侯成立张德权宋璇陈丽王振宇李欣李铮康宁丁楷
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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