Hemoglobin coloring agent and preparation method thereof
A technology of hemoglobin and colorant, applied in the field of hemoglobin colorant and its preparation, can solve problems such as poor stability, hemoglobin is sensitive to light, limit the development of processing and application, achieve good stability, improve stability, and increase practicability and application-wide effects
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[0027] The invention provides a method for preparing a hemoglobin colorant, comprising: step 1, extracting hemoglobin aqueous solution from fresh blood of livestock and poultry; step 2, adding arginine or lysine to the hemoglobin aqueous solution to obtain a mixed solution, Wherein, the ratio of arginine or lysine in the mixed solution is 4-12 mg / mL, and the pH value is adjusted to 9.5-12; step 3, drying the mixed solution. As an antioxidant, arginine or lysine can protect hemoglobin, prevent hemoglobin from being oxidized to methemoglobin during the drying process, maintain the divalent state of iron, and enable it to maintain a bright red color. The invention determines the optimal process condition for preparing hemoglobin with high stability, and provides a basis for large-scale production and processing.
[0028] In a preferred embodiment, in the preparation method of the hemoglobin coloring agent, in the second step, when arginine or lysine is added to the hemoglobin aqu...
Embodiment 1
[0043] The hemoglobin colorant of embodiment one low concentration arginine (arginine concentration is 4mg / mL)
[0044] (1) Blood collection: After slaughtering the sheep that passed the quarantine, the blood was collected in a container previously equipped with 5% anticoagulant, and the volume ratio of blood and anticoagulant was 1:10 and quantitatively mixed;
[0045] (2) Centrifugation: centrifuge the anticoagulated blood at 6000g for 30min at 4°C, and collect the red blood cells in the lower layer;
[0046] (3) Crude extraction of hemoglobin: RBCs were washed 3 to 5 times with normal saline, ultrasonic power was 250w, frequency was 25kHz, ultrasonic treatment was for 30min, centrifugal force was 8000g, and temperature was 4°C-6°C.
[0047] (4) Refined hemoglobin extraction: Hemoglobin crude extract was heated in a 60°C water bath for 30 minutes, cooled and then centrifuged with a centrifugal force of 8000g and a temperature of 4°C to 6°C.
[0048] (5) Treatment of hemoglo...
Embodiment 2
[0050] The hemoglobin colorant of embodiment two high-concentration arginine (arginine concentration is 12mg / mL)
[0051] Wherein the steps are the same as in Embodiment 1. In step (5), the concentration of added arginine is 12 mg / mL.
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