Rapid detection kit for IgG blood group antibody titer
A detection kit and blood type antibody technology, applied in the field of blood type detection, can solve the problems of long detection time, complicated production process, inconvenient operation, etc., and achieve the effect of convenient storage and portability, good specificity, and elimination of interference
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Embodiment 1
[0033] A kit for rapid detection of IgG blood group antibody titer, comprising standard erythrocyte suspension, washing solution and detection test strips.
[0034] like figure 1 and 2 As shown, the detection test strip 1 includes a sample application part 11, a reaction part 12 and a water absorption part 13 which are sequentially contacted from upstream to downstream, and also includes a first sealing strip 110, a second sealing strip 120 and a third sealing strip. Belt 130; the material of sample loading portion 11 is a porous polymer material (preferably glass fiber) with water conductivity and hydrophilicity, the material of water absorption portion 13 is cotton pulp paper, and the material of reaction portion 12 is nitrocellulose membrane, acetic acid Cellulose membrane, polyethylene membrane or nylon membrane, more preferably a backed nitrocellulose membrane with a capillary flow rate of 40-90 sec / 4cm width.
[0035] The sample application part 11 is covered on the re...
Embodiment 2
[0042] Identify IgG anti-A (B), anti-D in the serum (plasma) of pregnant women with the IgG blood group antibody titer rapid detection kit of embodiment 1, specifically comprise the steps:
[0043] (1) Adding samples: add 5 μL of serum (syrup) of pregnant women to be tested in sample adding well 183;
[0044] (2) Add standard erythrocyte suspension: add 5 μ LAB&RhD type 10% (v / v) erythrocyte suspension to add erythrocyte well 182;
[0045] (3) Add rinsing solution: add 100 μL of rinsing solution in hole 181 for adding rinsing solution;
[0046] (4) Interpretation of results: if image 3 As shown, after 3 minutes of interpretation, the detection point 121 and the control point 122 are all red, which is a positive reaction, indicating that the serum (serum) of pregnant women contains one, two or two of IgG anti-A, anti-B or anti-D. three kinds. If only the control point 122 is red, and the detection point 121 is not colored, it is a negative reaction, indicating that there is...
Embodiment 3
[0048] Identify the IgG anti-A (B) titer in the pregnant woman's serum (plasma) with the IgG blood group antibody titer quick detection kit of embodiment 1, specifically comprise the steps:
[0049] (1) Sample dilution: Dilute the serum (serum) of pregnant women to be tested with normal saline or 10mM PBS to 1:32, 1:64, 1:128, 1:256, etc.;
[0050] (2) Sample addition: Add 5 μL of the diluted sample to the sample addition hole 183;
[0051] (3) Add standard erythrocytes: add 5 μL (B) type 10% (v / v) erythrocyte suspension to add erythrocyte well 182;
[0052] (4) Add rinsing solution: add 100 μL of rinsing solution in hole 181 for adding rinsing solution;
[0053] (5) Interpretation of results: if image 3 As shown, after 3 minutes of interpretation, the detection point 121 and the control point 122 are all red, which is a positive reaction, and the reciprocal of the highest dilution of the positive result is the titer of the tested sample IgG anti-A (B).
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