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Rapid detection kit for IgG blood group antibody titer

A detection kit and blood type antibody technology, applied in the field of blood type detection, can solve the problems of long detection time, complicated production process, inconvenient operation, etc., and achieve the effect of convenient storage and portability, good specificity, and elimination of interference

Pending Publication Date: 2020-07-28
INTEC PROD INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But it still has many problems and deficiencies, such as, 1) the production process is complicated, artificial blood group antigen needs to be prepared, and tracer particles are needed for labeling; 2) the process is not mature, artificial blood group antigen is only a theoretical concept, and its practical application is almost impossible. Zero, specificity, affinity, and stability cannot be studied. 3) The result interpretation is not easy, and there are 3-100 lines in the result interpretation area, including many coated lines in the block area, detection area, and quality control area, etc., 4 ) is still inconvenient to operate. When there is no blocking zone, it is necessary to use traditional chemical methods to inactivate IgM antibodies in advance, and then measure the processed samples. 5) The detection time is relatively long and takes more than ten minutes, because the test strip is based on traditional The low pore size nitrocellulose membrane chromatography strip, and the result interpretation area located on the nitrocellulose membrane is very long, the sample passes for a long time, and the release of tracer particle-labeled substances also takes time. 6) The types of detection antibodies are limited, only Measures IgG anti-A and anti-B antibodies but not IgG anti-D

Method used

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  • Rapid detection kit for IgG blood group antibody titer
  • Rapid detection kit for IgG blood group antibody titer
  • Rapid detection kit for IgG blood group antibody titer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A kit for rapid detection of IgG blood group antibody titer, comprising standard erythrocyte suspension, washing solution and detection test strips.

[0034] like figure 1 and 2 As shown, the detection test strip 1 includes a sample application part 11, a reaction part 12 and a water absorption part 13 which are sequentially contacted from upstream to downstream, and also includes a first sealing strip 110, a second sealing strip 120 and a third sealing strip. Belt 130; the material of sample loading portion 11 is a porous polymer material (preferably glass fiber) with water conductivity and hydrophilicity, the material of water absorption portion 13 is cotton pulp paper, and the material of reaction portion 12 is nitrocellulose membrane, acetic acid Cellulose membrane, polyethylene membrane or nylon membrane, more preferably a backed nitrocellulose membrane with a capillary flow rate of 40-90 sec / 4cm width.

[0035] The sample application part 11 is covered on the re...

Embodiment 2

[0042] Identify IgG anti-A (B), anti-D in the serum (plasma) of pregnant women with the IgG blood group antibody titer rapid detection kit of embodiment 1, specifically comprise the steps:

[0043] (1) Adding samples: add 5 μL of serum (syrup) of pregnant women to be tested in sample adding well 183;

[0044] (2) Add standard erythrocyte suspension: add 5 μ LAB&RhD type 10% (v / v) erythrocyte suspension to add erythrocyte well 182;

[0045] (3) Add rinsing solution: add 100 μL of rinsing solution in hole 181 for adding rinsing solution;

[0046] (4) Interpretation of results: if image 3 As shown, after 3 minutes of interpretation, the detection point 121 and the control point 122 are all red, which is a positive reaction, indicating that the serum (serum) of pregnant women contains one, two or two of IgG anti-A, anti-B or anti-D. three kinds. If only the control point 122 is red, and the detection point 121 is not colored, it is a negative reaction, indicating that there is...

Embodiment 3

[0048] Identify the IgG anti-A (B) titer in the pregnant woman's serum (plasma) with the IgG blood group antibody titer quick detection kit of embodiment 1, specifically comprise the steps:

[0049] (1) Sample dilution: Dilute the serum (serum) of pregnant women to be tested with normal saline or 10mM PBS to 1:32, 1:64, 1:128, 1:256, etc.;

[0050] (2) Sample addition: Add 5 μL of the diluted sample to the sample addition hole 183;

[0051] (3) Add standard erythrocytes: add 5 μL (B) type 10% (v / v) erythrocyte suspension to add erythrocyte well 182;

[0052] (4) Add rinsing solution: add 100 μL of rinsing solution in hole 181 for adding rinsing solution;

[0053] (5) Interpretation of results: if image 3 As shown, after 3 minutes of interpretation, the detection point 121 and the control point 122 are all red, which is a positive reaction, and the reciprocal of the highest dilution of the positive result is the titer of the tested sample IgG anti-A (B).

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PUM

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Abstract

The invention discloses a rapid detection kit for IgG blood group antibody titer. The rapid detection kit comprises a standard red blood cell suspension, a flushing fluid and a detection test strip; the detection test strip comprises a sample adding part, a reaction part and a water absorption part which are sequentially arranged in a contact manner from the upstream side to the downstream side; the sample adding part is sequentially provided with a washing sample adding area, an erythrocyte sample adding area and a sample adding area from the upstream side to the downstream side; the reactionpart is provided with at least one detection point and a contrast point, wherein at least one detection point is coated and cured with an anti-human IgG antibody, and the contrast point is coated andcured with an anti-RBC antibody. When the kit is used, only one-step sample adding, red blood cell adding and flushing fluid adding are needed, and a result can be obtained after 3-5 minutes.

Description

technical field [0001] The invention belongs to the technical field of blood type detection, and in particular relates to a kit for rapid detection of IgG blood type antibody titer. Background technique [0002] Hemolytic disease of the newborn (HDN) is one of the most common acute hemolytic diseases in pediatrics. IgG antibody is the main type, and this IgG antibody can enter the fetus through the placenta, destroying fetal red blood cells and causing HDN. In severe cases, miscarriage, premature delivery, stillbirth, hemolytic jaundice, severe anemia, and nuclear jaundice may develop. It can cause early death of infants or leave survivors with irreversible neurological sequelae, bringing great pain to children and their families. Among the human blood group systems discovered so far, hemolytic disease of the newborn caused by ABO blood group incompatibility is the most common, followed by the Rh blood group system, and HDN caused by other blood group systems is relatively ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80G01N33/558
CPCG01N33/80G01N33/558
Inventor 胡晶高
Owner INTEC PROD INC
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