Mixture for improving memory and preparation method and application thereof

A mixture and memory technology, applied in the field of molecular biology, to achieve the effect of improving cognition, reducing side effects, and reducing drug dependence

Active Publication Date: 2017-04-26
江苏豪思睦可生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the specific components of these substances and the methods of separation and acquisition have not been reported yet.

Method used

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  • Mixture for improving memory and preparation method and application thereof
  • Mixture for improving memory and preparation method and application thereof
  • Mixture for improving memory and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0067] In the second aspect, the present invention provides a method for preparing the mixture HC4201612, the mixture is prepared from plasma extraction, and the preparation method sequentially includes the following steps: plasma collection, low temperature filtration, low temperature ultrafiltration, cold ethanol precipitation, SD inactivation , centrifugation, anion exchange, dialysis, concentration steps. details as follows:

[0068] Step 1. Plasma collection:

[0069] The blood is collected with an anticoagulant tube, and the supernatant is collected by centrifugation to obtain plasma; preferably, the centrifugation speed is 500-1500g, the centrifugation time is 10-30min, and the centrifugation temperature is 0-4°C.

[0070] Exemplarily, the above-mentioned centrifugal speed can be any value among 500g, 1000g, 1250g, 1500g, etc. or any value range between any two, and the above-mentioned centrifugation time can be any value among 10min, 15min, 20min, 30min, etc. or any t...

Embodiment 1

[0124] The present embodiment is the preparation method of mixture HC4201612, and this method comprises the following steps:

[0125] (1) Collection of plasma

[0126] The blood of healthy people is obtained from blood donation in the hospital (the blood collection tube is an anticoagulant tube). During the blood collection process, the blood collection tube is shaken to prevent blood coagulation. Put the blood collection tube into the centrifuge, set the rotation speed to 1500g, centrifuge for 15 minutes, and the centrifugation temperature is 4°C. Then use a pipette to carefully aspirate the supernatant, which is the collected human plasma.

[0127] (2) Low temperature filtration:

[0128] The collected plasma was added to a filter with a filter membrane pore size of 0.22 microns, and a peristaltic pump was used to apply a pressure of 10 MPa for filtration. During the process, the temperature of the entire filtration device was controlled at 0-4° C. to obtain a filtrate.

...

Embodiment 2

[0144] In this example, the mixture HC4201612 prepared in Example 1 was identified by SDS-PAGE denaturing gel electrophoresis. The identification method includes the following steps:

[0145] (1) Take 2 microliters from the above-mentioned mixture HC4201612, and measure its absorbance under ultraviolet 280 nm, thereby calculating the protein concentration of the mixture HC4201612.

[0146] (2) Take a certain volume of the mixture HC4201612 and mix it with 1 microliter of 5× protein loading buffer (purchased from Beijing Lamboride Biotechnology Co., Ltd., Cat. No. D621), which is the sample to be electrophoresed. The sample contains 10 micrograms of protein.

[0147] (3) Heat the electrophoresis sample to 100°C, heat for 20 minutes to denature the protein, then immediately put the sample on ice, wait for 5 minutes and start running SDS-PAGE denatured reducing gel (the preparation method of the SDS denatured reducing gel is as follows : 30 (w / v)% acrylamide Acr-Bis (purchased ...

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Abstract

The invention discloses a mixture for improving memory and a preparation method and application thereof. The mixture includes a variety of proteins and small molecules, the mixture has 6 bands which are clearly visible by naked eyes during SDS-PAGE denaturing gel electrophoresis, and the molecular weights of the bands are as follows: 25kD, 55kD, 68kD, 73kD, 100kD and 160kD. The preparation method includes plasma collection, low temperature filtration, low temperature ultrafiltration, cold ethanol precipitation, SD inactivation, centrifugation separation, anion exchange, dialysis and enrichment steps. The mixture can be used to prevent, improve and treat Alzheimer's disease, and has the function of strengthening memory.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a plasma isolate, in particular to a mixture for improving memory and a preparation method thereof, the mixture can be used for preventing, improving and treating senile dementia, and has the effect of enhancing memory effect. Background technique [0002] Alzheimer's disease, also known as Alzheimer's Disease (AD), is a neurodegenerative disease characterized by memory loss, cognitive impairment, and personality changes. The disease manifests as mild memory impairment in the early stage. As the disease progresses, the patient's cognitive ability gradually declines, intelligence declines, emotional and language disorders appear, and eventually the nervous system function is severely damaged, leading to death. [0003] There are many hypotheses about the specific pathogenesis of AD: such as cholinergic neuron hypothesis, Aβ toxicity hypothesis, Tau protein hypothesis, insulin hypoth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/16A61K38/17A61P25/28
CPCA61K35/16A61K38/1709
Inventor 崔文宏张丽丽孔毅荣郝敬雨石浩威任园园
Owner 江苏豪思睦可生物科技有限公司
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