Photosynthetic bacterium strain and its use in prevention and treatment on clubroot of cruciferous crops
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A technology for photosynthetic bacteria and cruciferous crops, applied in the directions of application, bacteria, fungicides, etc., can solve the problems of clubroot disease prevention and control that have not been reported in relevant literature, and achieve the effects of safe use, efficient control and reduction of incidence.
Active Publication Date: 2017-04-26
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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[0005] At present, there is no relevant literature report on the successful ap...
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Embodiment 1
[0020] 1. Experimental materials and methods
[0021] 1.1 Source of strain
[0022] The rhizosphere of mildly affected plants in Guanghan clubroot was seriously affected.
[0023] 1.2 Medium
[0024] The medium formula is: peptone 10g, yeast extract 5g, sodium chloride 10g, water 1000mL, pH adjusted to 5.0.
[0025] 1.3 Storage of strains
[0026] The strains were inoculated in 25% glycerol, stored at -70°C or liquid nitrogen, and transplanted once a month.
[0027] 1.4 Identification of strains
[0028] The bacterial DNA was extracted, and the 16S rDNA sequence was analyzed by PCR method, and the gene sequence is shown in the sequence list. The resulting sequence was compared on the NCBI website, and it was named Photosynthetic Bacteria GH-1.
[0029] Application of 1.5 strains in controlling clubroot of cruciferous crops
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Abstract
The invention relates to a photosynthetic bacterium strain for preventing and treating clubroot of cruciferous crops and its application and belongs to the technical field of plant protection. The photosynthetic bacterium belongs Lampropedia sp, is named as photosynthetic bacterium GH-1, is preserved in the China center for type culture collection on November 25, 2016 and has a preservation number of CCTCC NO: M 2016680. The strain has high ability to effectively prevent and treat clubroot of cruciferous crops, can be used for preventing and treating clubroot of cruciferous crops, reduces clubroot morbidity to about 10%, observably reduces a plant disease index, effectively improves a crop yield, has a yield increasing rate of 150% or more, is environmentally friendly and safe, does not produce residues and broadens the application field of photosynthetic bacteria.
Description
technical field [0001] The invention relates to a photosynthetic bacterium and its application in preventing and treating clubroot of cruciferous crops, and belongs to the technical field of microorganism and plant biological control. Background technique [0002] Cruciferous crops include rapeseed and bulk vegetables, such as cabbage, cabbage, cauliflower, kohlrabi, mustard greens, radishes, etc., as well as special crops such as isatis root and horseradish, which are the most widely planted and most commonly eaten vegetables in my country. , Guangxi, Sichuan, Anhui, Hunan, Jiangxi, Fujian, Jiangsu, Yunnan, Xinjiang, Tibet, Liaoning, Shandong, Shanghai, Beijing and Chongqing are distributed. However, cruciferous crops are susceptible to clubroot. Clubroot occurs in more than 20 million mu in my country every year, and it is extremely difficult to prevent and control, which seriously affects farmers' production, life and economic benefits. [0003] At present, the main contr...
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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/01
CPCA01N63/10C12N1/205C12R2001/01
Inventor 刘勇伍文宪黄小琴张蕾尹全周西全向运佳
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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