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Pharmaceutical composition for wound treatment containing Divine Comedy extract

A kind of Divine Comedy extract and extract technology, applied in the field of wound treatment, can solve the problem that the wound healing effect of Divine Comedy is not yet known, and achieve excellent wound healing effect

Active Publication Date: 2021-07-13
KOREA INST OF ORIENTAL MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the wound-healing effects of the Divine Comedy are not known

Method used

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  • Pharmaceutical composition for wound treatment containing Divine Comedy extract
  • Pharmaceutical composition for wound treatment containing Divine Comedy extract
  • Pharmaceutical composition for wound treatment containing Divine Comedy extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the preparation of Divine Comedy extract

[0045] The Shenqu extract was prepared based on the method shown below.

[0046] 1-1: Preparation of Divine Comedy Extract

[0047] Add 50g of crushed Divine Comedy to 1L of water and soak for 1 hour. Then, the solution was subjected to hot water extraction for 4 hours using a Daewoong extractor (DWP-5000M, Incheon, Korea). After hot water extraction, the resulting solution was concentrated under reduced pressure and freeze-dried to obtain 100 g of Shenqu extract.

[0048] 1-2: Preparation of longan meat (Longanae arillus) extract

[0049] As a comparison group, longan meat was used. Add 50g of chopped longan meat into 1L of water and soak for 1 hour. Then, the solution was subjected to hot water extraction for 4 hours using a Daewoong extractor (DWP-5000M, Incheon, Korea). After hot water extraction, the obtained solution was concentrated under reduced pressure and freeze-dried to obtain 100 g of longan mea...

Embodiment 2

[0054] Example 2: Confirmation of Induction Activity of Human Keratinocytes (HaCat) Proliferation

[0055] In order to confirm the wound healing effect of the Shenqu extract prepared in Example 1, the effect on keratinocyte proliferation was confirmed in advance.

[0056] Specifically, the 4.0×10 4 cells / mL HaCat cells were transferred to 96-well plates and cultured in DMEM (Dulbecco's Modified Eagle's Medium) containing 10% (v / v) fetal bovine serum (FBS) for 24 hours. The medium was treated with the Shenqu extract having a concentration of 50 μg / mL and 100 μg / mL, respectively, and then culture was further continued for 24 or 48 hours. For the comparison group, the culture medium was processed for comparison with the longan meat and Kochia extract with a concentration of 50 μg / mL, and for the positive control group, with the jujube extract with a concentration of 500 μg / mL, for The medium was treated for comparison, and for the negative control group, the medium was treated ...

Embodiment 3

[0059] Example 3: Confirmation of wound healing activity

[0060] After Example 2, in order to confirm the wound healing effect of the Shenqu extract, keratinocytes were wounded and the wound healing activity was confirmed.

[0061] Specifically, the 2.5×10 5 Cells / mL of HaCat were transferred to 6-well plates and cultured in DMEM containing 10% (v / v) fetal bovine serum (FBS) for 24 hours, then, induced on the HaCat cell monolayer by p200 pipette tip "Abrasion damage". The "abrasion-injured" HaCat cell layer was treated with the Shenqu extract having a concentration of 50 μg / mL and 100 μg / mL, and then further cultured for 24 or 48 hours. For the comparison group, the cell monolayer was treated with longan meat and Kochia extracts having a concentration of 50 μg / mL for comparison, and for the positive control group, the cell monolayer was treated with the jujube extract having a concentration of 150 μg / mL For comparison, and for the negative control group, the cell monolayer...

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Abstract

The present invention relates to a pharmaceutical composition, a quasi-drug composition and a health-care functional food composition for wound treatment, as well as a wound treatment method. The compositions all contain the extract of Divine Comedy as an active ingredient, and the method includes performing the combination on an individual The step of administering the composition or applying the composition to the skin of an individual. The composition containing the extract of Divine Comedy of the present invention induces the proliferation of keratinocytes, thereby exhibiting an excellent wound treatment effect, and thus can be effectively used in medicines for wound treatment including skin external preparations.

Description

technical field [0001] The present invention relates to a pharmaceutical composition, a quasi-drug composition and a health-care functional food composition for wound treatment, as well as a wound treatment method. The compositions all contain the extract of Divine Comedy as an active ingredient, and the method includes performing the combination on an individual administration of the composition or application of the composition to the skin of an individual. Background technique [0002] The skin, which acts as a shield to protect the body, is the body's first line of defense against disease. The epidermis provides a barrier against microbial invasion. Therefore, in the treatment of wounds, burns, abrasions, and other skin-related injuries, the primary goal is rapid closure and wound healing aimed at preventing infection. In general, wound healing is a complex process consisting of 3 phases: inflammation, proliferation and remodeling. The first phase is coagulation to ac...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/899A61K36/481A61K36/736A61P17/02
CPCA61K9/0014A61K36/481A61K36/736A61K36/899A61K2236/19A61P17/00A61P17/02A61K2300/00
Inventor 马珍烈李正珍权慧恩金爱英金兑洙赵元卿
Owner KOREA INST OF ORIENTAL MEDICINE
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