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A composite gel system for promoting wound healing and its preparation method and application

A compound gel and wound healing technology, which is applied in the fields of pharmaceutical formulation, medical science, drug delivery, etc., can solve the problems of poor automatic disintegration performance, achieve good biocompatibility, good therapeutic effect, and shorten the healing time

Active Publication Date: 2021-06-29
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to improve the stability of GSNO in wound healing, patent CN109276577A (a nitric oxide nanocomposite hydrogel and its preparation method and application) discloses a nitric oxide nanocomposite hydrogel, which uses sodium alginate as gel Gel matrix, NO donor GSNO as the main drug, and acrylic resin Eudragit RS PO as the drug-loading material to encapsulate GSNO to achieve stable protection of NO donor GSNO, but the system has poor automatic disintegration performance on the wound surface

Method used

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  • A composite gel system for promoting wound healing and its preparation method and application
  • A composite gel system for promoting wound healing and its preparation method and application
  • A composite gel system for promoting wound healing and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The preparation of embodiment 1 composite gel GSNO / GG-cl-Im-Ba

[0026] Prepare Im-Ba solution: weigh Im-Ba dry sample, prepare Im-Ba solution with a concentration of 1% m / v, and adjust to pH 8-9 with 1M NaOH solution;

[0027] Configuration of guar gum / GSNO solution: add 850 μL deionized water to a round bottom flask, vacuumize, fill with nitrogen, under nitrogen protection, add 36 mg guar gum powder and 0.9 mg GSNO, stir with 500 g magnetic force until completely dissolved, Get guar gum / GSNO solution;

[0028] Take 300 μL of guar gum / GSNO solution, add 150 μL of Im-Ba solution, and stir magnetically for 30 s under the protection of nitrogen to obtain the ROS-sensitive composite gel GSNO / GG-cl-Im-Ba loaded with GSNO.

Embodiment 2

[0029] Example 2 Preparation of composite gel GSNO / tEVs / GG-cl-Im-Ba

[0030] Prepare Im-Ba solution: weigh the dried Im-Ba sample, prepare Im-Ba solution with a concentration of 1% m / v, and adjust the pH to 8-9 with 1M NaOH solution;

[0031] Preparation of tEVs suspension: Exosomal tEVs were extracted from 80ml 4T1 cell culture supernatant by ultracentrifugation, and dissolved in 200μL PBS buffer solution to make the concentration of tEVs 1.19×10 10 Each / ml tEVs suspension was stored at 4°C for later use;

[0032] Configuration of guar gum / GSNO solution: add 850 μL deionized water to a round bottom flask, vacuumize, fill with nitrogen, under nitrogen protection, add 36 mg guar gum powder and 0.9 mg GSNO, stir with 500 g magnetic force until completely dissolved, Get guar gum / GSNO solution;

[0033] Take 300 μL of guar gum / GSNO solution, add 200 μL of step-tEVs suspension, under nitrogen protection, magnetically stir and mix to obtain a mixed solution; then add 150 μL of Im-...

Embodiment 3

[0034] Example 3 Biocompatibility Evaluation of New Composite Gel GSNO / tEVs / GG-cl-Im-Ba Material

[0035] 4-week-old male C57BL / 6 (C57) mice were divided into three groups of parallel controls, 3 mice in each group, and 0.5ml of compound gel GSNO / tEVs / GG-cl-Im- Ba, 4 days later, tissue sections around the subcutaneous injection of the legs were taken, and the biocompatibility of the GSNO / tEVs / GG-cl-Im-Ba hydrogel in vivo was evaluated by hematoxylin-eosin (HE) staining.

[0036] The results of HE staining showed that there were no inflammatory cells in the leg muscles of mice injected with the composite gel GSNO / tEVs / GG-cl-Im-Ba, indicating that the composite gel GSNO / tEVs / GG-cl-Im-Ba Low immunogenicity and high safety.

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Abstract

The invention belongs to the technical field of skin wound repair, and specifically relates to a composite gel system for promoting skin wound healing, a preparation method and an application thereof, wherein the composite gel system is loaded with tumor exosomes tEVs and / or nitroso Composition of thiol glutathione GSNO. The composite gel system provided by the present invention uses the gel material GG-cl-Im-Ba as a drug carrier, co-carries NO donor nitrosothiol glutathione GSNO and / or tumor exosome tEVs, and forms a composite The gel system can not only improve the stability of GSNO, but also has the characteristics of carrier disintegration and therapeutic drug release in response to the microenvironment of the wound site. The composite gel system can also promote wound healing through the synergistic effect of GSNO and tEVs, especially For the healing of high glucose refractory wounds.

Description

technical field [0001] The invention belongs to the technical field of gel materials, and in particular relates to a composite gel system for promoting wound healing, a preparation method and an application. Background technique [0002] Trauma is the damage caused by normal skin or tissue under the action of external injury factors. Common traumas include surgical incision, abrasions, burns, skin donor sites, decubitus ulcers, vascular ulcers of lower extremities, diabetic ulcers, and other non-healing wounds. Wounds, etc., have a major impact on the function of the body. Unlike normal wound healing, the wounds of refractory diabetic foot ulcers experience a longer inflammatory period, in which the pathological microenvironment shows an increase in ROS, and ROS in excess At a high concentration, it prevents the transition from inflammation to the proliferation phase, which is not conducive to the proliferation and migration of fibroblasts, thereby hindering the formation of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L26/00
CPCA61L26/0023A61L26/0057A61L26/0066A61L26/008A61L2300/30A61L2300/412A61L2400/06C08L5/00
Inventor 南文滨王帆郝永伟陈红丽张其清
Owner XINXIANG MEDICAL UNIV
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