Rapid propagation method of pteridophyte spores

Abstract

The invention belongs to the technical field of pteridophyte spore propagation, and discloses a rapid propagation method of pteridophyte spores. The method comprises the steps of S1, collecting mature spores of a pteridophyte, and removing impurities; S2, preparing MS macroelements, gibberellin, carbendazim and sodium alginate into a gel liquid, and then stirring the gel liquid with the spores evenly to prepare a spore gel liquid; S3, extracting the spore gel liquid, dropwise adding the spore gel liquid into a CaCl2 solution for curing reaction to form spore balls, and taking out and cleaning the spore balls; S4, using peat soil and perlite as a matrix, watering the matrix sufficiently one day before sowing, and a tray is stacked at the bottom for water retention; S5, sowing the spore ball on the surface of the matrix of a plug, wherein one ball is sowed in every plug, there is no need to cover the soil, and a moisture retention cover is covered; S6, maintenance management. According to the rapid propagation method of the pteridophyte spores, fine spores are prepared into artificial seeds, by covering nutrient substances and a bacteriostatic agent, the growth of the spores is accelerated, and thus the spores are grown into mature gametophytes in a short period; meanwhile, the spore density is effectively controlled, transplanting is not needed, and the spores is directly cultivated into seedlings.

Description

technical field [0001] The invention belongs to the technical field of fern breeding, in particular to a method for rapid propagation of fern spores. Background technique [0002] Ferns, also known as ferns, are primitive vascular plants and a taxonomic group between bryophytes and seed plants. It is a higher spore plant with obvious generation replacement, and the sporophyte of the asexual generation is dominant. [0003] The spore propagation of ferns is a shortcut for rapid breeding of seedlings. It has many advantages such as simplicity, large reproduction, low cost, and simplicity. It is the most important way of fern propagation. The usual practice is to spread the spores evenly on the substrate, and transplant after the spores develop into heart-shaped gametophytes. However, this method usually has the following disadvantages: (1) the amount of spores is large and the seedlings are less; (2) the development of spores is slow; (3) artificial spreading often leads to ...

AI Technical Summary

Problems solved by technology

However, this method usually has the following disadvantages: (1) the amount of spores is large and the seedlings are less; (2) the development of spores is slow; (3) artificial spreading often leads to excessive density, which is not conducive to the development of prophyllum; Bacterial or fungal infection will spread rapidly, which will easily lead to the death of the prothallus and stop its development into seedlings.

[0004] Artificial seed technology is to contain the somatic embryos or tissues that can develop into complete plants in the in vitro culture of plants in the shell that contains nutrients and has protective functions, and can develop into granules that can develop into seedlings under suitable conditions. It is used in the tissue culture of various crops, economic crops and rare species, but there is no report on the technology of fern artificial seed propagation