Method for marking larimichthys crocea by using alizarin red

A technology of large yellow croaker and alizarin red, applied in application, fish farming, climate change adaptation, etc., can solve problems such as clogging of large yellow croaker gills, death, etc., achieve strong tolerance, increase effective concentration, and improve discrimination The effect of accuracy

Active Publication Date: 2017-05-10
INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a method for marking large yellow croaker with alizarin red. By deploying artificial seawater, the method avoids the influence of calcium ions on the dissolution of alizarin red and the generation of precipitation, and improves the alizarin red. The effective concentration and dipping effect solve the problem that the suspended sediment easily clogs the gills of large yellow croakers and causes a large number of deaths

Method used

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  • Method for marking larimichthys crocea by using alizarin red

Examples

Experimental program
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Effect test

Embodiment 1

[0026] (1) Use distilled water to prepare 2000mg / L stock mother liquor of alizarin red, and use an air stone to aerate for 12 hours to fully dissolve alizarin red; dilute the mother liquor to 200mg / L with tap water after 48 hours of aeration; use iodine-free Refined salt (commercially available), MgSO 4 , MgCl 2 and KCl to adjust the salinity to 15‰, to obtain artificial seawater dyeing solution (addition amount is to add 12.3g of iodine-free refined salt per liter of dyeing solution, MgSO 4 1.0g, MgCl 2 1.6g, KCl 0.3g). The dyeing liquor is aerated for 12 hours, and the pneumatic filter cotton is used to absorb the sediment (2 per cubic meter of water body), and NaHCO 3 The pH of the solution was adjusted to 8.2.

[0027] (2) Dyeing at 6m 3 in the breeding pond. Soak 12,000 large yellow croaker fry with an average length of 5.15±0.75cm to be dyed (about 5cm, pre-adapted to seawater with a salinity of 15‰ without alizarin red for 48h) in the above-mentioned artificial ...

Embodiment 2

[0030] (1) prepare the alizarin red reserve mother liquor of 2000mg / L with distilled water, alizarin red is fully dissolved with air stone strong aeration 12h during preparation; In three 300L dyeing barrels, dilute the mother liquor with tap water after aeration 48h respectively to 100, 200, 300mg / L; add 12.3g of iodine-free refined salt, MgSO 4 1.0g, MgCl 2 1.6g, KCl 0.3g, to obtain an artificial seawater dyeing solution with a salinity of 15%, aerate for 12 hours, use a pneumatic filter cotton for each barrel to absorb sediment, and use NaHCO 3 The pH of the solution was adjusted to 8.2.

[0031] (2) Put 500 large yellow croaker fry with a total length of 4.68±0.82cm into each bucket for soaking and dyeing. The soaking time is 24 hours. 3 The pH of the solution was adjusted to keep it between 7.8 and 8.2; after dyeing, the fry were transferred to natural sea water for rinsing for 24 hours to remove floating color on the body surface.

[0032] The dyeing and marking eff...

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Abstract

The invention relates to a method for marking larimichthys crocea by using alizarin red. The method comprises the steps that 1, an alizarin red is prepared for mother solution preparation, a mother solution is diluted to reach application concentration, then the salinity is regulated with refined salt and the like to reach 1.5% or above, and an artificial seawater dye liquor is obtained; 2, larimichthys crocea fry to be dyed is soaked in the dye liquor for dyeing, the fry is transferred to natural seawater for rinsing after dyeing is completed, and then larimichthys crocea marked with the alizarin red is obtained. By the adoption of the method, the influence of calcium ions on alizarin red dissolution and precipitation are avoided, the effectively concentration and a dip dyeing effect of the alizarin red are improved, and the problem of massive dead due to the fact that suspending precipitates easily block the gills of the larimichthys crocea is solved.

Description

technical field [0001] The invention belongs to the field of large yellow croaker marking, in particular to a method for marking large yellow croaker with alizarin red. Background technique [0002] Marking recapture is the most commonly used method to evaluate the effect of fish proliferation and release. This method needs to mark the fish before releasing to distinguish the released group from the wild group. Currently commonly used labeling methods include physical labeling, biomolecular labeling and chemical labeling. In these methods, physical marking will cause certain damage to the fish body, and it is only suitable for marking larger fish bodies, and the marking cost is high, and it needs to be operated one by one, so it is not suitable for large-scale marking and releasing. Biomolecular marker detection procedures are too complicated. Chemical labeling mainly refers to the formation of stable chemical fluorescent labels on the operculum, otoliths and other parts t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/95
CPCY02A40/81
Inventor 贾超峰张志勇许津刘海林张志伟陈淑吟张曹进吴国均祝斐
Owner INST OF OCEANOLOGY & MARINE FISHERIES JIANGSU
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