A three-dimensional liquid chromatography separation system

Abstract

The invention relates to a three-dimensional high performance liquid chromatography separation system, comprising a high performance liquid chromatography pump, an automatic sampler, a liquid chromatography column, a column thermostat, a two-position multi-way valve, a trapping column, a gradient mixer and a detector . The present invention realizes the simplification of the complex sample system by introducing the first-dimensional high-performance liquid chromatography pump and the first-dimensional liquid chromatography column, combined with subsequent comprehensive two-dimensional liquid chromatography separation, realizes the excellent separation ability of the three-dimensional high-performance liquid chromatography system . Its characteristic is that the first dimension selects the appropriate chromatographic column stationary phase to realize the pre-separation of the complex sample system. The second dimension separation is performed at low flow rates, coordinated with the third dimension separation speed. The third dimension uses short columns to achieve fast gradient separations at high flow rates. The separation of each dimension is connected by a trapping column, and a diluent is used to assist compound trapping. The invention realizes efficient separation of complex systems such as plants, environment, blood samples, urine samples, tissues and other samples by selecting different chromatographic column stationary phases and mobile phase combinations.

Description

technical field [0001] The invention belongs to a high performance liquid chromatography separation system, in particular to a three-dimensional ultra high performance liquid chromatography separation system. Background technique [0002] With the development of separation technology, exploring and separating components in complex sample systems has become a hot research field. Multidimensional liquid chromatography is a separation technology developed based on this research hotspot. Compared with the traditional one-dimensional liquid chromatography analysis method, the multidimensional liquid chromatography technology can effectively improve the resolution of complex sample component separation by increasing the peak capacity. Due to technical limitations, two-dimensional liquid chromatography systems are currently the most common. Depending on the component of interest, 2D-LC methods include comprehensive 2D and heart-cutting modes. Comprehensive two-dimensional liquid...

AI Technical Summary

Problems solved by technology

The interface technology based on the sample loop is the most commonly used interface technology. However, in order to match the analysis speed of the second dimension, the volume of the sample loop, that is, the injection volume of the second dimension, is usually very large, resulting in serious solvent effects.

The interface technology based on the trapping column can effectively avoid this problem by removing the solvent while retaining the compound, but due to the incompatibility of the solvent, the loss of the compound during the trapping process is the biggest drawback of this interface technology

Dwell mode can avoid the impact of second-dimensional separation speed on first-dimensional analysis, but comprehensive two-dimensional analysis using this interface technology is time-consuming

[0004] The composition of compounds in actual samples is very complex and their properties vary greatly

For different first-dimensional fractions, the use of isocratic or the same gradient conditions in the second-dimensional separation often cannot be well separated

Although the gradient mode used in the second dimension can improve the resolution of the method to a certain extent, this gradient mode is not only difficult to construct, but also less feasible for a two-dimensional liquid chromatography system with better orthogonality.

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