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Colloidal gold card reader for detecting mycotoxins in livestock farms

A colloidal gold card reader and mycotoxin technology, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve problems such as difficult to effectively control, long detection time, unable to deal with in time, and achieve the effect of avoiding errors and benefiting stability

Inactive Publication Date: 2017-05-10
上海源紊新能源科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The harm of mycotoxins to animals is a worldwide problem. China is a large agricultural country, but the large proportion of China's breeding industry is farmer's free-range breeding or small-scale farm breeding. The scale is scattered, and the level of prevention and control of mycotoxin diseases and production management capabilities are backward. , in the event of an epidemic, it is difficult to effectively control
The traditional experimental detection of mycotoxins also has technical problems such as complex procedures and long detection time. After the detection results come out, the products have been sold or transferred, which cannot be processed in time, and cannot meet the needs of on-site detection in a timely, efficient and fast manner.

Method used

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  • Colloidal gold card reader for detecting mycotoxins in livestock farms
  • Colloidal gold card reader for detecting mycotoxins in livestock farms

Examples

Experimental program
Comparison scheme
Effect test

experiment example

[0041] The colloidal gold test paper of the present invention is compared with the HPLC method to detect the same mycotoxin in different samples. The colloidal gold test paper is detected as an experimental group, and the HPLC method is a control group. Each treatment is repeated 3 times, and the experimental results are averaged. The results are as follows: Table 1 shows.

[0042] Table 1 Comparison data table of colloidal gold test paper and HPLC method detection results

[0043]

[0044] According to the comparison results of the data after the mass spectrometry detection sample and the colloidal gold quantitative reagent strip, the detection result of the colloidal gold is very close to the detection result of the actual sample, and the accuracy of the sample detection result and the real result is controlled at about 96%; The average deviation CV% of repeated test results is controlled within 15%, indicating that the accuracy of the test results of the colloidal gold r...

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Abstract

The invention particularly discloses a colloidal gold card reader for detecting mycotoxins in livestock farms. The colloidal gold card reader comprises a colloidal gold test paper card reading cavity and a colloidal gold test paper image collecting device, wherein the image collecting device comprises a microprocessor, the microprocessor comprises a communication module, an image sensor, a cold light source and a display system, and the microprocessor is connected and communicated with an intelligent terminal through the communication module; mycotoxin colloidal gold test paper corresponding to built-in programs of the microprocessor is required to be placed in the colloidal gold test paper card reading cavity. According to the colloidal gold card reader, color developing results of the colloidal gold test paper are collected by the image sensor, so that detection result errors caused by errors produced by manual evaluation can be avoided. The detection results of the same batch are processed by one evaluation system and are calculated according to a preset internal standard curve, so that stability of the detection results is facilitated.

Description

technical field [0001] The invention relates to the technical field of colloidal gold test paper, in particular to a colloidal gold card reader for detecting mold in farms. Background technique [0002] Colloidal gold is synthesized by chloroauric acid (HAuC14) under the action of reducing agents such as white phosphorus, ascorbic acid, tannic acid, etc., to synthesize gold particles of a certain size, and become a stable colloidal state due to electrostatic interaction, forming a negatively charged hydrophobic colloid solution , It becomes a stable colloidal state due to electrostatic interaction, so it is called colloidal gold. Immunogold labeling technology mainly utilizes the high electron density of gold particles. Dark brown particles can be seen under the microscope at the gold-labeled protein binding site. When these markers gather in large quantities at the corresponding ligands, red or pink can be seen with the naked eye. Spots, thus used in qualitative or semi-qu...

Claims

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Application Information

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IPC IPC(8): G01N33/53
CPCG01N33/5302
Inventor 不公告发明人
Owner 上海源紊新能源科技有限公司
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