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A tissue culture method for promoting glycyrrhizic acid accumulation in licorice adventitious roots

A technology of glycyrrhizic acid and licorice, which is applied in the field of traditional Chinese medicine licorice tissue culture, and achieves the effects of short growth cycle, increased content of glycyrrhizic acid compounds, and low production cost

Inactive Publication Date: 2018-11-30
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] So far, there is no report on the use of anti-allergic protein LSP1 to promote the accumulation of glycyrrhizic acid in adventitious roots of Glycyrrhizae

Method used

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  • A tissue culture method for promoting glycyrrhizic acid accumulation in licorice adventitious roots
  • A tissue culture method for promoting glycyrrhizic acid accumulation in licorice adventitious roots

Examples

Experimental program
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Effect test

Embodiment 1

[0019] The solid medium is formulated as follows: add sucrose to 1 / 2 MS medium to make the final mass concentration 3.5%, add indolebutyric acid to make the final concentration 1.0mg / L, add agar to make the final mass concentration 0.65% , adjust pH=5.9.

Embodiment 2

[0021] The solid medium is formulated as follows: add sucrose to 1 / 2 MS medium to make the final mass concentration 4%, add indolebutyric acid to make the final concentration 1.0mg / L, add agar to make the final mass concentration 0.6% , adjust pH=5.8.

Embodiment 3

[0023] The solid medium is formulated as follows: add sucrose to 1 / 2 MS medium to make the final mass concentration 3%, add indolebutyric acid to make the final concentration 1.0mg / L, add agar to make the final mass concentration 0.7% , adjust pH=6.0.

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Abstract

The invention discloses a tissue culture method for promoting accumulation of glycyrrhizic acid in liquorice adventitious roots. The method comprises the following steps: (1) inoculating the roots, stems or leaves of liquorice into a solid culture medium, inducing the growth of the liquorice adventitious roots, inoculating the liquorice adventitious roots into a liquid culture medium and culturing for 33-37 days; (2) adding anti-allergic protein LSP1 into the liquid culture medium of the liquorice adventitious roots which are cultured for 33-37 days in the step (1), enabling the final concentration of the anti-allergic protein LSP1 to be 25mg / L-200mg / L, keeping culturing for 4-6 days and harvesting. The method disclosed by the invention has the advantages that the operation is simple, the content of glycyrrhizic acid compounds in the liquorice adventitious roots can be obviously increased, the growth period is short, the production cost is low, and the industrial mass production of the glycyrrhizic acid becomes convenient.

Description

technical field [0001] The invention relates to a tissue culture method for promoting accumulation of glycyrrhizic acid in adventitious roots of licorice by using anti-sensitive protein LSP1, belonging to the field of tissue culture of traditional Chinese medicine licorice. Background technique [0002] Glycyrrhizic acid (Glycyrrhizic acid) is a type E saponin, which is the most abundant saponin in licorice. As the most important active ingredient in licorice, glycyrrhizic acid mainly has the following uses: (1) Pharmaceutical industry: Glycyrrhizic acid has anti-inflammatory, liver-protecting, anti-virus, anti-tumor, interferon-inducing agents, and immune function regulation. It is clinically used in the prevention and treatment of chronic hepatitis, bronchitis, AIDS and various cancers. (2) Food and cigarette industry: Glycyrrhizic acid is a health-care sweetener with high sweetness and low calorie value. Its sweetness is more than 250 times that of sucrose. It has disad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 高文远李建丽王娟李金鑫
Owner TIANJIN UNIV
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