Tissue culture method for promoting accumulation of glycyrrhizic acid in liquorice adventitious roots
A technology of glycyrrhizic acid and licorice is applied in the field of traditional Chinese medicine licorice tissue culture to achieve the effects of improving the content of glycyrrhizic acid compounds, low production cost and short growth cycle
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Embodiment 1
[0019] The solid medium is formulated as follows: add sucrose to 1 / 2 MS medium to make the final mass concentration 3.5%, add indolebutyric acid to make the final concentration 1.0mg / L, add agar to make the final mass concentration 0.65% , adjust pH=5.9.
Embodiment 2
[0021] The solid medium is formulated as follows: add sucrose to 1 / 2 MS medium to make the final mass concentration 4%, add indolebutyric acid to make the final concentration 1.0mg / L, add agar to make the final mass concentration 0.6% , adjust pH=5.8.
Embodiment 3
[0023] The solid medium is formulated as follows: add sucrose to 1 / 2 MS medium to make the final mass concentration 3%, add indolebutyric acid to make the final concentration 1.0mg / L, add agar to make the final mass concentration 0.7% , adjust pH=6.0.
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