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Bile extract as well as extracting method and application thereof

A technology of extract and gallbladder, which is applied in the field of biochemical drugs, can solve the problems of cell canceration, unsatisfactory effect, and high price, and achieve high effect, easy industrial production, and low cost

Inactive Publication Date: 2017-05-17
张志强 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the growth factors used in research at home and abroad are all obtained by gene recombination technology, so the price is very expensive. For example, the price of EGF is as high as 2000 yuan / mg
In addition, the current research is to observe the effect of a single growth factor on the wound surface, and its effect is not very satisfactory, and there are doubts that a single growth factor stimulates cell proliferation and causes cell canceration

Method used

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  • Bile extract as well as extracting method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] In a sterile environment at 4°C, wash the fresh gallbladder repeatedly with physiological saline, then cut a small opening with medical scissors to take out the bile, put it into a container, and leave the gallbladder body for later use.

Embodiment 2

[0054] In a sterile environment at 4°C, remove the fatty tissue from the gallbladder body in Example 1, wash it with physiological saline, peel off the gallbladder mucosa and put it in a container, and put the gallbladder with the gallbladder mucosa removed in another container for later use .

Embodiment 3

[0056] In a sterile environment at 4° C., the bile prepared in Example 1 was centrifuged at 8000 rpm / min for 20 min on a low-temperature high-speed centrifuge to collect the precipitate.

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Abstract

The invention relates to bile extract as well as an extracting method and application thereof. The bile extract containing a plurality of types of effective components is obtained by taking bile tissues of mammals as raw materials and extracting, separating and concentrating through simple steps of freeze thawing, freeze centrifuging, carrying out micro-filtration, carrying out ultra-filtration, carrying out reverse osmosis, freeze drying and the like. The bile extract provided by the invention has a remarkable treatment effect on various skin wounds, gastric ulcer, fundus hemorrhage and the like.

Description

technical field [0001] The invention belongs to the field of biochemical medicines, and in particular relates to bile extract, its extraction method and application. Background technique [0002] Various long-term difficult-to-heal wounds (ulcers) caused by diseases are common diseases in clinical surgery, which make the body lose its natural barrier, suppress the immune function of the body, and cause other diseases at the same time. The disease has a high incidence rate and difficult healing, which seriously affects the quality of life of patients. Treating wounds, that is, wound repair, is one of the important topics to be further studied in the medical field. [0003] Cell growth factor, also known as cell growth regulator, is a kind of polypeptide with strong physiological activity, which can regulate the growth, proliferation and differentiation of cells. Cell growth factors can stimulate the growth of macrophages, epithelial cells, endothelial cells, fibroblasts and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/413A23L33/10A61P27/02A61P7/04A61P1/04A61P17/00A61P17/02A61K8/98A61Q19/00
Inventor 张志强刘宣亚刘建斌
Owner 张志强
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