Nucleic acid extraction, amplification and detection integrated mechanical device

Abstract

The invention relates to a nucleic acid extraction, amplification and detection integrated mechanical device which comprises a kit, a kit heater, a motor control mechanism, an integrated circuit board and a bar code reader, wherein the kit heater is driven by a motor, and a magnet is wrapped inside the kit heater; the right end part of a screw rod extending outside the body of the kit and the right end part of a pressing plate shaft extending outside the body of the kit are both connected with the motor, and a sampling needle of the kit is controlled by the motor to move transversely and upwards and downwards; and the kit is inserted into an instrument and is recognized and treated through the bar code reader, and analysis results are directly summarized into a computer. All reagents for nucleic acid extraction, amplification and detection are all put into the kit in advance, so that the possibility of pollution can be avoided; a high-concentration PCR (Polymerase Chain Reaction) product is sealed inside the kit, and thus pollution sources are also effectively controlled; the problem of pollution can be solved, so that users can use the device safely, and the problems of space shortage and too high foundation construction cost can be solved; and molecular diagnosis nucleic acid extraction, amplification and detection are integrated, and automation and sealing are achieved.

Description

[0001] (1) Technical field [0002] The invention belongs to the technical field of biomedical equipment, and particularly relates to a mechanical device integrating nucleic acid extraction, amplification and detection. [0003] (2) Background technology [0004] There are three main steps in molecular diagnosis: nucleic acid extraction, amplification and detection. There are many methods for each step, and each method has different reagents to choose from. For each method, some manufacturers produce different automated instruments. Therefore, a challenge for clinical users is how to select and integrate these Reagents and instruments make each step have the most optimized results. [0005] An optimal solution for molecular differential diagnosis should be: extraction, amplification, detection, trinity; fully automatic, fully enclosed, pollution-proof, and triathlon. [0006] Necessity of amplification: The purpose of amplification is to increase the signal-to-noise ratio. All diagnos...

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Problems solved by technology

Solving the problem of pollution not only reassures users, but also solves the p

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