Lentinan with liver cancer resisting and immunity improving actions and preparation method thereof

Abstract

The invention discloses lentinan with liver cancer resisting and immunity improving actions and a preparation method thereof. The preparation method comprises the steps: crushing dried shiitake mushroom fruit bodies, and adding water, cellulase, neutral proteinase, pectinase and papain into the crushed shiitake mushroom fruit bodies for enzymolysis; (2) carrying out high-pressure homogenization treatment, so as to obtain homogenate; (3) filtrating the homogenate, carrying out depressurized concentration on filtrate, adding an aqueous solution of ethanol, carrying out standing, carrying out centrifugation, and drying precipitates, thereby obtaining the lentinan. Shiitake mushrooms are pretreated by using a compound enzyme so as to hydrolyze cell walls and change texture of the shiitake mushrooms, and a high-pressure homogenizer is then used for carrying out short-time treatment so as to thoroughly and uniformly mix effective ingredients with a solvent, so that the extraction efficiency is high, active ingredients are not destroyed, and the preparation process is simple, convenient, safe and non-toxic. The prepared lentinan has the total sugar content of 90% or more and the molecular weight range of 1.82*10<2>KDa to 4.46*10<2>KDa and has the advantages of improving immunity, being remarkable in liver cancer resisting effect and improving the survival quality of tumor-bearing mice.

Description

technical field

[0001] The invention relates to a preparation method of lentinan having the function of resisting liver cancer and improving immunity, and belongs to the technical application field of medicine and health care products. Background technique

[0002] Liver cancer is a common type of cancer with high morbidity and mortality. Surgical treatment and radiotherapy and chemotherapy are the main means of treating liver cancer at present. However, surgical treatment has a poor prognosis and is prone to relapse; chemical drugs will bring great toxic and side effects because of their killing effect on normal cells, which will bring great pain to patients. Therefore, scientists are working hard to find more efficient and low-toxic anticancer agents from natural products. Studies have shown that long-term low immunity is also one of the important reasons for cancer, and improving the immune level of the body is a key means of tumor treatment. Therefore, it is very nece...

Examples

Embodiment 1

[0022] A preparation method of lentinan having the effect of resisting liver cancer and improving immunity, comprising the steps of:

[0023] (1) Enzymolysis treatment: take dried fruiting bodies of mushrooms, crush them, add 20 times the mass of deionized water, add cellulase, neutral protease, pectinase and papain; at 45°C, the enzymolysis time is 3 hours; The mass ratio of the mushroom fruiting body, cellulase, neutral protease, pectinase and papain is 40:0.6:0.12:0.4:0.5;

[0024] (2) High-pressure homogenization treatment: process the mushroom enzymolysis solution obtained in step (1) with a high-pressure homogenizer under a pressure of 100 MPa for 3 times, each time for 20 minutes, to obtain a homogeneous solution;

[0025] (3) Extraction of lentinan: the homogeneous liquid obtained in step (2) is filtered, and the filtrate is concentrated under reduced pressure to 1 / 6 of the volume of the filtrate to obtain a concentrated solution, and the volume concentration of adding...

Embodiment 2

[0027] A preparation method of lentinan having the effect of resisting liver cancer and improving immunity, comprising the steps of:

[0028] (1) Enzymolysis treatment: take the dried mushroom fruiting bodies, crush them, add 40 times the mass of deionized water, add cellulase, neutral protease, pectinase and papain; enzymolysis at 55°C for 4 hours; The mass ratio of mushroom fruiting body, cellulase, neutral protease, pectinase and papain is 20:0.5:0.05:0.2:0.3;

[0029] (2) High-pressure homogenization treatment: process the shiitake mushroom enzymolysis solution obtained in step (1) with a high-pressure homogenizer under a pressure of 50 MPa for 1 time, each time for 30 minutes, to obtain a homogeneous solution;

[0030] (3) Extraction of lentinan: filter the homogeneous liquid obtained in step (2), concentrate the filtrate under reduced pressure to 1 / 4 of the volume of the filtrate to obtain a concentrated solution, add a volume concentration of 2.5 volumes of the concentr...

Embodiment 3

[0032] A preparation method of lentinan having the effect of resisting liver cancer and improving immunity, comprising the steps of:

[0033] (1) Enzymolysis treatment: take the dried mushroom fruiting bodies, crush them, add 10 times the mass of deionized water, add cellulase, neutral protease, pectinase and papain; enzymolysis at 55°C for 2 hours; The mass ratio of mushroom fruiting body, cellulase, neutral protease, pectinase and papain is 50:1:0.15:0.5:0.4;

[0034] (2) High-pressure homogenization treatment: process the shiitake mushroom enzymatic solution obtained in step (1) with a high-pressure homogenizer for 3 times at a pressure of 80 MPa, each time for 10 minutes, to obtain a homogeneous solution;

[0035] (3) Extraction of lentinan: the homogeneous liquid obtained in step (2) is filtered, and the filtrate is concentrated under reduced pressure to 1 / 5 of the volume of the filtrate to obtain a concentrate, and the volume concentration of adding 4 times the volume of...

technical field

[0001] The invention relates to a preparation method of lentinan having the function of resisting liver cancer and improving immunity, and belongs to the technical application field of medicine and health care products. Background technique

[0002] Liver cancer is a common type of cancer with high morbidity and mortality. Surgical treatment and radiotherapy and chemotherapy are the main means of treating liver cancer at present. However, surgical treatment has a poor prognosis and is prone to relapse; chemical drugs will bring great toxic and side effects because of their killing effect on normal cells, which will bring great pain to patients. Therefore, scientists are working hard to find more efficient and low-toxic anticancer agents from natural products. Studies have shown that long-term low immunity is also one of the important reasons for cancer, and improving the immune level of the body is a key means of tumor treatment. Therefore, it is very nece...

Examples

Embodiment 1

[0022] A preparation method of lentinan having the effect of resisting liver cancer and improving immunity, comprising the steps of:

[0023] (1) Enzymolysis treatment: take dried fruiting bodies of mushrooms, crush them, add 20 times the mass of deionized water, add cellulase, neutral protease, pectinase and papain; at 45°C, the enzymolysis time is 3 hours; The mass ratio of the mushroom fruiting body, cellulase, neutral protease, pectinase and papain is 40:0.6:0.12:0.4:0.5;

[0024] (2) High-pressure homogenization treatment: process the mushroom enzymolysis solution obtained in step (1) with a high-pressure homogenizer under a pressure of 100 MPa for 3 times, each time for 20 minutes, to obtain a homogeneous solution;

[0025] (3) Extraction of lentinan: the homogeneous liquid obtained in step (2) is filtered, and the filtrate is concentrated under reduced pressure to 1 / 6 of the volume of the filtrate to obtain a concentrated solution, and the volume concentration of adding...

Embodiment 2

[0027] A preparation method of lentinan having the effect of resisting liver cancer and improving immunity, comprising the steps of:

[0028] (1) Enzymolysis treatment: take the dried mushroom fruiting bodies, crush them, add 40 times the mass of deionized water, add cellulase, neutral protease, pectinase and papain; enzymolysis at 55°C for 4 hours; The mass ratio of mushroom fruiting body, cellulase, neutral protease, pectinase and papain is 20:0.5:0.05:0.2:0.3;

[0029] (2) High-pressure homogenization treatment: process the shiitake mushroom enzymolysis solution obtained in step (1) with a high-pressure homogenizer under a pressure of 50 MPa for 1 time, each time for 30 minutes, to obtain a homogeneous solution;

[0030] (3) Extraction of lentinan: filter the homogeneous liquid obtained in step (2), concentrate the filtrate under reduced pressure to 1 / 4 of the volume of the filtrate to obtain a concentrated solution, add a volume concentration of 2.5 volumes of the concentr...

Embodiment 3

[0032] A preparation method of lentinan having the effect of resisting liver cancer and improving immunity, comprising the steps of:

[0033] (1) Enzymolysis treatment: take the dried mushroom fruiting bodies, crush them, add 10 times the mass of deionized water, add cellulase, neutral protease, pectinase and papain; enzymolysis at 55°C for 2 hours; The mass ratio of mushroom fruiting body, cellulase, neutral protease, pectinase and papain is 50:1:0.15:0.5:0.4;

[0034] (2) High-pressure homogenization treatment: process the shiitake mushroom enzymatic solution obtained in step (1) with a high-pressure homogenizer for 3 times at a pressure of 80 MPa, each time for 10 minutes, to obtain a homogeneous solution;

[0035] (3) Extraction of lentinan: the homogeneous liquid obtained in step (2) is filtered, and the filtrate is concentrated under reduced pressure to 1 / 5 of the volume of the filtrate to obtain a concentrate, and the volume concentration of adding 4 times the volume of...

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