Method for preserving arbuscular mycorrhizal fungi

Abstract

The invention discloses a method for preserving arbuscular mycorrhizal fungi. The moisture in propagules of the arbuscular mycorrhizal fungi is fully reduced before cryopreservation of strains. The method comprises the following steps: ventilating and drying at normal temperature, and reducing the majority of moisture in a matrix material so that the moisture content is reduced to below 8%; and then reducing the moisture content in the propagules by utilizing silica gel drying in a glass dryer so that the moisture content of a matrix is reduced to below 5%. The moisture content of the propagule material is reduced by two steps so that the moisture content in arbuscular mycorrhizal fungi spores and mycelial cells is slowly reduced, thus being beneficial to maintain the vitality of the strains and simultaneously avoiding the damage of ice crystals to the cells during the rapid freezing. Through the experimental comparison, the activity of strains of the arbuscular mycorrhizal fungi can be effectively preserved. Compared with a pot culture inoculating method and an cryopreservation method, the method does not occupy a culture greenhouse, does not need the regular artificial inoculation and does not need a strain protection agent and a liquid nitrogen preservation material, the operation is simple, and the material resources and manpower are saved.

Description

technical field [0001] The invention relates to the field of soil microorganisms, in particular to a method for preserving arbuscular mycorrhizal fungi. [0002] technical background [0003] Arbuscular mycorrhizal fungi are mycorrhizal fungi that can form symbionts with plant roots and play an important role in plant growth and development. At present, many mycorrhizal products have been used in fields such as promoting plant growth, nutrient absorption, and preventing and controlling diseases and insect pests. Arbuscular mycorrhizal fungi with excellent traits usually need to be preserved for later research and use. Due to the different physiological characteristics of arbuscular mycorrhizal fungi and other microorganisms, there are differences in preservation methods from other microorganisms. At present, the preservation methods of arbuscular mycorrhizal fungi mainly include pot culture preservation method, normal temperature preservation method and ultra-low temperatur...

AI Technical Summary

Problems solved by technology

Ultra-low temperature preservation is a method of combining bacterial propagules with protective agents and storing them in a -80°C refrigerator or liquid nitrogen. This method can preserve the bacterial agents for a long period of time, but this method is cumbersome and costly to maintain. High, not suitable for general institutions

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