A Strain yzs020 suitable for Cultivation of Substitute Materials
A technology for cultivating and cultivating rhizoma rhododendron is applied in the field of yzs020 strains of rhododendron rhododendron, which can solve the problems of slow growth of mycelium, disordered strains, large quality fluctuation, etc., and achieves high content of mycelial polysaccharide and neat fruiting. Uniform, cold-resistant effect
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Embodiment 1
[0025] Embodiment 1 of the present invention: the starting strains in the following examples are all wild strains with different habitats or cultivated strains with large differences in growth. There are 40 strains in total, 5 of which are cultivated strains, and the rest are wild strains, as shown in Table 1.
[0026] Table 1 The tested Dictyophora strains and sources
[0027]
[0028] Embodiment 1 of the present invention: natural breeding of Dictyophora yzs020 strain
[0029] The natural breeding process of Dictyophora edulis strain yzs020 of the present invention comprises the following steps:
[0030] 1) Selection of natural strains: 40 strains were selected, of which 5 were cultivated types and 35 were wild types. All these strains were preserved by the Fungi Department of Guizhou Crop Variety Resources Research Institute.
[0031] 2) Preliminary screening of breeding characteristics of mother species
[0032] Through the analysis of the growth and growth speed of ...
Embodiment 2
[0055] Example 2 of the present invention: Morphological characteristics and biological characteristics of Dictyophora yzs020 strain
[0056] Morphological characteristics:
[0057] The hyphae are white and dense, the aerial hyphae are developed, multi-branched, separated, double-nucleated, and have lock-like joints. After the mycelia are stimulated by strong light or mechanical stimulation, and when the stamina of the medium is insufficient, red discoloration will occur. This is red. The unique properties of Dictyophora ( image 3 ).
[0058] The fruiting body belongs to abdominal fungi, and has obvious receptacle, stipe, fungus skirt, cap and spores after shell breaking. Among them, the receptacle is milky white, with dot-like scales on the surface, and appears red or even purple after being stimulated by strong light or machinery; the stipe is white, cylindrical, 13-18 cm long, 3.5-5 cm in diameter, spongy, hollow; the fungus skirt White, brittle, drooping from the top o...
Embodiment 3
[0062] Embodiment 3 of the present invention: Physicochemical properties of the yzs020 strain of Dictyophora edulis
[0063] Analysis of polysaccharide content:
[0064] The polysaccharide content of strain yzs020 is 1.23%, which is higher than that of cultivated strains. Please refer to Figure 4 .
[0065] Extracellular Enzyme Activity Analysis:
[0066] The filter paper enzyme activity was 5.63U / g, the CMC enzyme activity was 15.18U / g, the hemicellulase activity was 6.34U / g, and the laccase activity was 3.59U / g. The content of filter paper enzyme and CMC enzyme is relatively high, and the content of hemicellulase and laccase is relatively low, which can well decompose the cellulose in the matrix material, and is suitable for the cultivation of straw substitute material. Please refer to the specific differences Figure 5 .
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